Long-term preservation of potato leafroll virus, potato virus S, and potato spindle tuber viroid in cryopreserved shoot tips

2018 ◽  
Vol 102 (24) ◽  
pp. 10743-10754 ◽  
Author(s):  
Jing-Wei Li ◽  
Min-Rui Wang ◽  
Hai-Yan Chen ◽  
Lei Zhao ◽  
Zhen-Hua Cui ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Spindle Tuber Viroid ◽  
Potato Leafroll Virus ◽  
Shoot Tips ◽  
Potato Virus S ◽  
Leafroll Virus ◽  
Long Term Preservation

scholarly journals Multiple DNA markers for evaluation of resistance against Potato virus Y, Potato virus S and Potato leafroll virus

2018 ◽  
Vol 54 (No. 1) ◽  
pp. 30-33 ◽  
Author(s):  
M. Naderpour ◽  
L. Sadeghi
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Simultaneous Detection ◽  
Potato Leafroll Virus ◽  
Potato Seed ◽  
Breeding Programs ◽  
Potato Virus S ◽  
Sequence Characterized Amplified Region ◽  
Leafroll Virus ◽  
Resistance To Viruses

Molecular markers within or close to genes of interest play essential roles in marker-assisted selection. PCR-based markers have been developed for numerous traits in different plant species including several genes conferring resistance to viruses in potato. In the present work, rapid and reliable approaches were developed for the simultaneous detection of Ryadg and Ry-fsto, Ns, and PLRV.1 genes conferring resistance to Potato virus Y, Potato virus S and Potato leafroll virus, respectively, on the basis of previously published and newly modified markers. The sequence characterized amplified region (SCAR) markers for Ryadg, Ns and PLRV1 and the newly modified cleaved amplified polymorphic sequences (CAPS) marker for Ry-fsto were amplified in one PCR reaction which could simply characterize Ryadg and PLRV.1 resistance. Additional digestion of amplicons with EcoRV and MfeI for genotyping the Ry-fsto and Ns resistance genes, respectively, was needed. The effectiveness of genotyping in triplex and tetraplex PCRs was tested on 35 potato varieties used for potato seed production and breeding programs.  

Resistance to potato leafroll virus infection and accumulation in potato cultivars, and the effects of previous infection with other viruses on expression of resistance

1993 ◽  
Vol 44 (8) ◽  
pp. 1891 ◽  
Author(s):  
CR Wilson ◽  
RAC Jones
Keyword(s):  
Potato Virus ◽  
Potato Virus X ◽  
Potato Leafroll Virus ◽  
Potato Cultivars ◽  
Infected Leaf ◽  
Potato Virus S ◽  
Breeding Lines ◽  
Infection Pressure ◽  
Previous Infection ◽  
Leafroll Virus

A selection of potato cultivars and breeding lines was evaluated for presence of resistance to infection with potato leafroll virus (PLRV) via viruliferous aphid vectors ( IR) and/or resistance to accumulation of PLRV antigen ( AR) in infected leaf tissue. Cultivars Aracy, Delcora, Omega and Spunta, and breeding lines BR63.15 and B71.240.2 carried both IR and AR , Bismark, Serrana INTA and L/T1 had alone and Delaware had AR alone. The other cultivars tested had neither. Within both the resistant and susceptible classes for AR, the level of PLRV antigen accumulation achieved varied with cultivar. Previous infection with potato virus X (PVX) or potato virus S (PVS) either alone or together did not diminish the expression of IR or AR. However, the presence of PVX sometimes significantly increased the accumulation of PLRV in susceptible cv. Desiree and this effect was most pronounced in mature leaves of older plants. In contrast, presence of PVX in susceptible cv. Desiree did not increase the numbers of plants becoming infected with PLRV. Identifying potato genotypes with and IR AR will help the Australian potato industry to select cultivars which become infected with PLRV more slowly under conditions of high infection pressure and/or are suitable for use as parental lines in breeding virus-resistant cultivars.

scholarly journals Diagnóstico serológico y molecular del Potato leafroll virus (PLRV) en tubérculos-semilla de papa en Antioquia, Colombia

2015 ◽  
Vol 65 (2) ◽  
pp. 204-210 ◽  
Author(s):  
Mauricio Esteban Mesa Medina ◽  
Martha Isabel González Ramírez ◽  
Pablo Andrés Gutiérrez Sánchez ◽  
Mauricio Alejandro Marín Montoya
Keyword(s):  
Solanum Tuberosum ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Potato Leafroll Virus ◽  
Rt Pcr ◽  
Potato Virus S ◽  
Leafroll Virus ◽  
Das Elisa

<p>Las virosis son uno de los problemas fitosanitarios más limitantes para la producción de papa, siendo los virus Potato leafroll virus (PLRV), Potato virus Y (PVY), Potato virus S (PVS) y Potato ye-llow vein virus (PYVV) los que generan mayores pérdidas económicas en la región andina. Gene-ralmente estos virus son transmitidos por insectos hemípteros y por tubérculos-semilla. En esta investigación se evaluó la presencia y distribución del PLRV en tubérculos de <em>Solanum tuberosum</em> variedad Diacol-Capiro y S. phureja variedad Criolla-Colombia obtenidos en Antioquia, utilizando pruebas serológicas y moleculares. Mediante DAS-ELISA se evaluaron 128 muestras consisten-tes en tres tipos de tejidos (piel, yemas latentes y brotes), mientras que con RT-PCR en tiempo real (RT-qPCR), se utilizó un subgrupo de 12 muestras de cada variedad. Utilizando DAS-ELISA, el PLRV se encontró en el 3,1% y 10,1% de las muestras de Diacol-Capiro y Criolla-Colombia, respectivamente; siendo evidente una mayor detección del virus en la piel de los tubérculos de ésta última variedad. Las pruebas de RT-qPCR detectaron el PLRV en 83,3% y 91,6% de las 12 muestras de Diacol-Capiro y de Criolla-Colombia, respectivamente; con valores de Ciclo umbral (CT) entre 17,72 y 30,59. La naturaleza de los amplicones obtenidos por RT-qPCR fue confirmada por secuenciación como parte de una región codificante de la cápside del PLRV (Identidad=99%-100%). Estos resultados enfatizan en la necesidad de utilizar tubérculo-semilla certificados por su sanidad viral en los cultivos de papa del país y de fortalecer dichos programas a partir del empleo de técnicas de detección altamente sensibles como RT-qPCR.</p>

scholarly journals Resistência de genótipos de batata ao vírus do enrolamento da folha da batata (PLRV) e ao vírus Y (PVY)

2004 ◽  
Vol 22 (3) ◽  
pp. 521-524 ◽  
Author(s):  
Julio Daniels ◽  
Arione da S. Pereira
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Rio Grande ◽  
Potato Leafroll Virus ◽  
Rio Grande Do Sul ◽  
Leafroll Virus ◽  
Das Elisa

O vírus do enrolamento da folha da batata (Potato leafroll virus, PLRV) e o vírus Y da batata (Potato virus Y, PVY) constituem as principais causas da degenerescência da batata-semente no Brasil. Com o objetivo de determinar, nas condições do Rio Grande do Sul, a resistência de campo de genótipos de batata à infecção por estes vírus, avaliaram-se, na presença de infectores, durante três plantios consecutivos de primavera, 20 cultivares e clones de batata. A detecção dos vírus foi efetuada por meio de testes sorológicos (DAS-ELISA). Pela análise de agrupamento os genótipos foram separados em três grupos para resistência ao PLRV (Elvira, Achat, Bintje, Monalisa, Monte Bonito, Panda e Araucária, resistentes; Baronesa, Asterix, Atlantic, 2CRI-1149-1-78, C-1226-35-80, Astrid, C-1714-7-94, A-1139-12-92, Macaca, Eliza e Santo Amor, suscetíveis; Catucha e Cristal, muito suscetíveis) e em quatro grupos para resistência ao PVY (Asterix, Astrid, Catucha, Cristal, Macaca, Monte Bonito, A-1139-12-92, C-1226-35-80 e C-1714-7-94, resistentes; Baronesa, Santo Amor, Monalisa, Panda e 2CRI-1149-1-78, resistentes intermediários; Bintje, Atlantic, Elvira e Araucária, suscetíveis; Achat e Eliza, muito suscetíveis).

Long-Term Preservation of Plant Viruses in Cryopreserved Shoot Tips

2021 ◽  
pp. 187-195
Author(s):  
Min-Rui Wang ◽  
Tianxing Pang ◽  
Ziqian Lian ◽  
Qiao-Chun Wang ◽  
Liying Sun
Keyword(s):  
Plant Viruses ◽  
Shoot Tips ◽  
Long Term Preservation

Molecular Characterization of Potato leafroll virus, Potato virus A, and Potato virus X Isolates from Potatoes in Alaskan Cities and Villages

2011 ◽  
Vol 12 (1) ◽  
pp. 39 ◽  
Author(s):  
Nancy L. Robertson ◽  
Jeffrey Smeenk ◽  
Jodie M. Anderson
Keyword(s):  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus X ◽  
Potato Leafroll Virus ◽  
First Report ◽  
Potato Virus A ◽  
Potato Viruses ◽  
The World ◽  
Leafroll Virus

Although all three viruses are commonly found in potatoes throughout the world, this is the first report of potato viruses from Alaska to be sequenced and molecularly analyzed for comparisons with known viruses. Accepted for publication 17 January 2011. Published 9 February 2011.

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