Resistance to potato leafroll virus infection and accumulation in potato cultivars, and the effects of previous infection with other viruses on expression of resistance

1993 ◽  
Vol 44 (8) ◽  
pp. 1891 ◽  
Author(s):  
CR Wilson ◽  
RAC Jones
Keyword(s):  
Potato Virus ◽  
Potato Virus X ◽  
Potato Leafroll Virus ◽  
Potato Cultivars ◽  
Infected Leaf ◽  
Potato Virus S ◽  
Breeding Lines ◽  
Infection Pressure ◽  
Previous Infection ◽  
Leafroll Virus

A selection of potato cultivars and breeding lines was evaluated for presence of resistance to infection with potato leafroll virus (PLRV) via viruliferous aphid vectors ( IR) and/or resistance to accumulation of PLRV antigen ( AR) in infected leaf tissue. Cultivars Aracy, Delcora, Omega and Spunta, and breeding lines BR63.15 and B71.240.2 carried both IR and AR , Bismark, Serrana INTA and L/T1 had alone and Delaware had AR alone. The other cultivars tested had neither. Within both the resistant and susceptible classes for AR, the level of PLRV antigen accumulation achieved varied with cultivar. Previous infection with potato virus X (PVX) or potato virus S (PVS) either alone or together did not diminish the expression of IR or AR. However, the presence of PVX sometimes significantly increased the accumulation of PLRV in susceptible cv. Desiree and this effect was most pronounced in mature leaves of older plants. In contrast, presence of PVX in susceptible cv. Desiree did not increase the numbers of plants becoming infected with PLRV. Identifying potato genotypes with and IR AR will help the Australian potato industry to select cultivars which become infected with PLRV more slowly under conditions of high infection pressure and/or are suitable for use as parental lines in breeding virus-resistant cultivars.

scholarly journals Multiple DNA markers for evaluation of resistance against Potato virus Y, Potato virus S and Potato leafroll virus

2018 ◽  
Vol 54 (No. 1) ◽  
pp. 30-33 ◽  
Author(s):  
M. Naderpour ◽  
L. Sadeghi
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Simultaneous Detection ◽  
Potato Leafroll Virus ◽  
Potato Seed ◽  
Breeding Programs ◽  
Potato Virus S ◽  
Sequence Characterized Amplified Region ◽  
Leafroll Virus ◽  
Resistance To Viruses

Molecular markers within or close to genes of interest play essential roles in marker-assisted selection. PCR-based markers have been developed for numerous traits in different plant species including several genes conferring resistance to viruses in potato. In the present work, rapid and reliable approaches were developed for the simultaneous detection of Ryadg and Ry-fsto, Ns, and PLRV.1 genes conferring resistance to Potato virus Y, Potato virus S and Potato leafroll virus, respectively, on the basis of previously published and newly modified markers. The sequence characterized amplified region (SCAR) markers for Ryadg, Ns and PLRV1 and the newly modified cleaved amplified polymorphic sequences (CAPS) marker for Ry-fsto were amplified in one PCR reaction which could simply characterize Ryadg and PLRV.1 resistance. Additional digestion of amplicons with EcoRV and MfeI for genotyping the Ry-fsto and Ns resistance genes, respectively, was needed. The effectiveness of genotyping in triplex and tetraplex PCRs was tested on 35 potato varieties used for potato seed production and breeding programs.  

Molecular Characterization of Potato leafroll virus, Potato virus A, and Potato virus X Isolates from Potatoes in Alaskan Cities and Villages

2011 ◽  
Vol 12 (1) ◽  
pp. 39 ◽  
Author(s):  
Nancy L. Robertson ◽  
Jeffrey Smeenk ◽  
Jodie M. Anderson
Keyword(s):  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus X ◽  
Potato Leafroll Virus ◽  
First Report ◽  
Potato Virus A ◽  
Potato Viruses ◽  
The World ◽  
Leafroll Virus

Although all three viruses are commonly found in potatoes throughout the world, this is the first report of potato viruses from Alaska to be sequenced and molecularly analyzed for comparisons with known viruses. Accepted for publication 17 January 2011. Published 9 February 2011.

scholarly journals One-step real-time multiplex reverse transcription-polymerase chain reaction assay with melt curve analysis for detection of potato leafroll virus, potato virus S, potato virus X, and potato virus Y

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Nobuya Onozuka ◽  
Takehiro Ohki ◽  
Norikuni Oka ◽  
Tetsuo Maoka
Keyword(s):  
Real Time ◽  
Seed Potato ◽  
Potato Virus ◽  
Potato Virus X ◽  
Virus Species ◽  
Potato Virus S ◽  
Melt Curve Analysis ◽  
One Step ◽  
High Throughput Detection ◽  
Leafroll Virus

Abstract Background Certification of seed potato as free of viruses is essential for stable potato production. Among more than 30 virus species infecting potato, potato leafroll virus (PLRV), potato virus S (PVS), potato virus X (PVX), and potato virus Y (PVY) predominate worldwide and should be the targets of a high-throughput detection protocol for seed potato quarantine. Results We developed an assay based on one-step real-time multiplex reverse transcription-polymerase chain reaction (mRT-PCR) with melt curve analysis for the four viruses and one internal control, potato elongation factor 1 alpha gene (EF1α). Virus-specific primers were derived from conserved regions among randomly selected representatives considering viral genomic diversity. Our assay simultaneously detected representative Japanese isolates of PLRV, O lineage of PVS, PVX, and NTN strain of PVY. The variability of melting temperature (Tm) values for each virus was confirmed using Japanese isolates, and virus species could be identified by the values of 87.6 for PLRV, 85.9 for PVX, 82.2 (Ordinary lineage) to 83.1 (Andean lineage) for PVS, and 79.4 (NA-N strain) to 80.5 (O strain and NTN strain) for PVY on average. The reliability of calculation was validated by comparing the calculated Tm values and measured Tm values and the values had a strong linear correlation (correlation of determination: R2 = 0.9875). Based on the calculated Tm values, representative non-Japanese isolates could also be identified by our assay. For removing false positives, two criteria were set for the evaluation of result; successful amplification was considered as 30.0 ≥ threshold cycle value, and the virus-specific peak higher than the EF1α-specific peak was considered as positive. According to these criteria, our assay could detect PLRV and PVS from 100-fold dilution of potato leaf homogenate and PVX and PVY from 1000-fold in a model assay. Conclusion This new high-throughput detection protocol using one-step real-time mRT-PCR was sensitive enough to detect viruses in a 100-fold dilution of singly-virus contaminated homogenate in a model assay. This protocol can detect the four viruses in one assay and yield faster results for a vast number of samples, and greatly save the labor for seed potato quarantine and field surveys.

scholarly journals Incidence and Distribution of Important Viral Pathogens in Some Iranian Potato Fields

Plant Disease ◽  
2007 ◽  
Vol 91 (5) ◽  
pp. 609-615 ◽  
Author(s):  
R. Pourrahim ◽  
Sh. Farzadfar ◽  
A. R. Golnaraghi ◽  
A. Ahoonmanesh
Keyword(s):  
Potato Virus ◽  
Alfalfa Mosaic Virus ◽  
Potato Virus X ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mixed Infections ◽  
Virus Infections ◽  
Potato Virus S ◽  
Leafroll Virus ◽  
Almost All ◽  
Biological Methods

From a total of 8,135 potato leaves collected from 132 fields in 11 provinces of Iran, the incidence and distribution of Alfalfa mosaic virus (AlMV), Eggplant mottled dwarf virus (EMDV), Potato leafroll virus (PLRV), Potato virus A (PVA), Potato virus M (PVM), Potato virus S(PVS), Potato virus X (PVX), Potato virus Y (PVY), and Tomato yellow fruit ring virus (TYFRV) were assessed using serological and biological methods. Based on enzyme-linked immunosorbent assay (ELISA) results, viruses in decreasing order of incidence in potato were PVS (35.9%), PVY (34.4%), PVA (27.0%), PVX (20.8%), PLRV (13.9%), PVM (9.0%), AlMV (7.0%), TYFRV (5.9%), and EMDV (5.1%). All 132 fields surveyed had some degree of virus infection, ranging from 28.8 to 98.6%, with an overall incidence of 75.2%. The highest and lowest incidence of virus infections among the surveyed provinces occurred in Kerman (93.2%) and Ardabil (56.7%), respectively. Overall, 25.0 and 50.2% of the collected potato samples had single or mixed infections, respectively. High levels of mixed infections were found between PVX and PVS (8.6%), and PVX and PVY (7.6%). Moreover, co-infection of samples with PVS and PVY, PVA and PVS, and PVA and PVY, the aphid-vectored virus/virus combinations, occurred at the highest incidence in almost all provinces surveyed, 15.3, 13.8, and 12.8%, respectively. In this study, Beet curly top virus was detected in symptomatic potato samples collected from some fields in the Kermanshah province.

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