Elucidating the impact of cottonseed hydrolysates on CHO cell culture performance through transcriptomic analysis

2020 ◽  
Vol 105 (1) ◽  
pp. 271-285
Author(s):  
Swetha Kumar ◽  
Venkata Gayatri Dhara ◽  
Linda D. Orzolek ◽  
Haiping Hao ◽  
Abbie J. More ◽  
...  
Keyword(s):  
Cell Culture ◽  
Transcriptomic Analysis ◽  
Cho Cell ◽  
Culture Performance ◽  
Cho Cell Culture ◽  
The Impact

Elucidating the Impact of CHO Cell Culture Media on Tryptophan Oxidation of a Monoclonal Antibody Through Gene Expression Analyses

2018 ◽  
Vol 13 (10) ◽  
pp. 1700254 ◽  
Author(s):  
Luhong He ◽  
Jairav X. Desai ◽  
Jinxin Gao ◽  
Laurie B. Hazeltine ◽  
Zhirui Lian ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Culture ◽  
Monoclonal Antibody ◽  
Culture Media ◽  
Cho Cell ◽  
Cell Culture Media ◽  
Gene Expression Analyses ◽  
Tryptophan Oxidation ◽  
Cho Cell Culture ◽  
The Impact

Improving culture performance and antibody production in CHO cell culture processes by reducing the Warburg effect

2018 ◽  
Vol 115 (9) ◽  
pp. 2315-2327 ◽  
Author(s):  
Maria Buchsteiner ◽  
Lake‐Ee Quek ◽  
Peter Gray ◽  
Lars K. Nielsen
Keyword(s):  
Cell Culture ◽  
Antibody Production ◽  
Warburg Effect ◽  
Cho Cell ◽  
Culture Performance ◽  
Cho Cell Culture ◽  
The Warburg Effect

scholarly journals Protein Hydrolysates from Flaxseed Oil Cake as a Media Supplement in CHO Cell Culture

Resources ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 59
Author(s):  
Marijan Logarušić ◽  
Višnja Gaurina Srček ◽  
Sara Berljavac ◽  
Andreja Leboš Pavunc ◽  
Kristina Radošević ◽  
...  
Keyword(s):  
Cell Culture ◽  
Preliminary Investigation ◽  
Cell Number ◽  
Flaxseed Oil ◽  
Protein Hydrolysates ◽  
Cho Cell ◽  
Protective Function ◽  
Cho Cell Culture ◽  
Media Supplements ◽  
The Impact

This is the first report about flaxseed protein hydrolysates applied as media supplements in CHO cell culture. The hydrolysates were produced by three separate enzymatic digestions of proteins isolated from flaxseed oil cake. The enzymes used were Alcalase, Neutrase, and Protamex, and the most efficient hydrolysis was achieved with Alcalase. The three hydrolysates were first tested as a partial substitute for serum in basal media in order to evaluate their effects on the adherent IgG-producing CHO cell line. The cells that grew in such media reached higher density than the cells in media supplemented with serum only. Consequently, the increased cell number improved the final IgG titer. In the next experiment, the impact of hydrolysates was evaluated in suspension CHO culture adapted to chemically defined media. In this preliminary investigation, the cells showed no response to the hydrolysate addition concerning the growth rate and productivity. Despite this outcome, we speculate that low molecular mass components in the hydrolysates, besides nutritive, may have a cell-protective function.

pH excursions impact CHO cell culture performance and antibody N-linked glycosylation

2018 ◽  
Vol 41 (12) ◽  
pp. 1731-1741 ◽  
Author(s):  
Rubin Jiang ◽  
Hao Chen ◽  
Sen Xu
Keyword(s):  
Cell Culture ◽  
Cho Cell ◽  
Culture Performance ◽  
Cho Cell Culture

scholarly journals Benchmarking of commercially available CHO cell culture media for antibody production

2015 ◽  
Vol 99 (11) ◽  
pp. 4645-4657 ◽  
Author(s):  
David Reinhart ◽  
Lukas Damjanovic ◽  
Christian Kaisermayer ◽  
Renate Kunert
Keyword(s):  
Cell Culture ◽  
Antibody Production ◽  
Culture Media ◽  
Cho Cell ◽  
Cell Culture Media ◽  
Cho Cell Culture
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