Correlation Analysis of Corporate Culture, Performance Management and Employee Development

In the process of development, the most important thing is to train talents and improve the comprehensive quality of employees. As the main content of talent training, corporate culture and performance management can be said to provide theoretical support for performance management.A good corporate culture environment can not only restrain the behavior of staff, but also improve the quality of performance management and promote the benign occurrence of enterprises in society. Based on this, this paper analyzes the correlation between corporate culture, performance management and employee development, and perfects the content and system of performance culture.

Amino acid feeding reduces ammonia production through rearrangement of metabolic fluxes in central carbon metabolism of CHO cells

Ammonia is a toxic byproduct of CHO cell metabolism, which inhibits cell growth, reduces cell viability, alters glycosylation, and decreases recombinant protein productivity. In an attempt to minimize the ammonium accumulation in cell culture media, different amino acids were added individually to the culture medium before the production phase to alleviate the negative effects of ammonium on cell culture performance. Among all the amino acids examined in this study, valine showed the most positive impact on CHO cell culture performance. When the cultured CHO cells were fed with 5 mM valine, EPO titer was increased by 25% compared to the control medium, and ammonium and lactate production were decreased by 23 and 26%, respectively, relative to the control culture. Moreover, the sialic acid content of the EPO protein in valine-fed culture was higher than in the control culture, most likely because of the lower ammonium concentration. Flux balance analysis (FBA) results demonstrated that the citric acid cycle was enriched by valine feeding. The analysis revealed that there might be a link between promoting tricarboxylic acid (TCA) cycle metabolism in valine-fed culture and reduction in lactate and ammonia accumulation. Furthermore, in valine-fed culture, FBA outcomes showed that alanine was excreted into the medium as the primary mechanism for reducing ammonium concentration. It was predicted that the elevated TCA cycle metabolism was concurrent with an increment in recombinant protein production. Taken together, our data demonstrate that valine addition could be an effective strategy for mitigating the negative impacts of ammonium and enhancing glycoprotein production in both quality and quantity.

Amino acid feeding reduces ammonia production through rearrangement of metabolic fluxes in central carbon metabolism of CHO cells

Ammonia is a toxic byproduct of CHO cell metabolism, which inhibits cell growth, reduces cell viability, alters glycosylation, and decreases recombinant protein productivity. In an attempt to minimize the ammonium accumulation in cell culture media, different amino acids were added individually to the culture medium before the production phase to alleviate the negative effects of ammonium on cell culture performance. Among all the amino acids examined in this study, valine showed the most positive impact on CHO cell culture performance. When the cultured CHO cells were fed with 5 mM valine, EPO titer was increased by 25% compared to the control medium, and ammonium and lactate production were decreased by 23 and 26%, respectively, relative to the control culture. Moreover, the sialic acid content of the EPO protein in valine-fed culture was higher than in the control culture, most likely because of the lower ammonium concentration. Flux balance analysis (FBA) results demonstrated that the citric acid cycle was enriched by valine feeding. The measurement of TCA cycle activity supported this finding. The analysis revealed that there might be a link between promoting tricarboxylic acid (TCA) cycle metabolism in valine-fed culture and reduction in lactate and ammonia accumulation. Furthermore, in valine-fed culture, FBA outcomes showed that alanine was excreted into the medium as the primary mechanism for reducing ammonium concentration. It was predicted that the elevated TCA cycle metabolism was concurrent with an increment in recombinant protein production. Taken together, our data demonstrate that valine addition could be an effective strategy for mitigating the negative impacts of ammonium and enhancing glycoprotein production in both quality and quantity.

PENGARUH GAYA KEPEMIMPINAN DAN KOMITMEN ORGANISASI TERHADAP KINERJA KARYAWAN PADA PT. BANK ACEH SYARIAH CABANG BENER MERIAH DAN CABANG TAKENGON MELALUI BUDAYA ORGANISASI SEBAGAI VARIABEL MODERASI

The research conducted to examine the influence of leadership style and organizational commitment and organizational culture on the employee performance and to examine the moderation effect of organizational culture. The sample of this research was 120 permanent employees at PT. Bank Aceh Syariah Branch of Bener Meriah and Branch of Takengon. The tool of analysis was Path analysis with SEM (stuctural equation modeling) method by using Amos. The research result partially indicated that leadership style and organizational commitment influenced positively and significantly on organizational culture, performance and organizational culture appearancely mediated leadership style with performance and organizational culture appearancely mediated organizational commitment with performance. Keyword: Leadership Style, Organizational Commitment, Organizational Culture, and Employee Performance.

PEMBENTUKAN POPULASI SINTETIS UNTUK PENINGKATAN KUALITAS GENETIK IKAN MAS

Benih ikan mas telah mengalami penurunan kualitas genetik yang menyebabkan penurunan performa fenotipik di lingkungan budidaya. Salah satu upaya perbaikan genetik adalah melalui pembentukan populasi sintetis yang merupakan penggabungan potensi genetik beberapa populasi plasma nutfah ikan mas. Penelitian ini bertujuan membentuk dan mengevaluasi performa genotipik dan fenotipik populasi sintetis ikan mas, yang merupakan penggabungan dari strain Rajadanu, Majalaya, Sutisna, Wildan, dan Sinyonya. Performa genotipik dievaluasi menggunakan metode mikrosatelit DNA, sedangkan performa fenotipik dievaluasi menggunakan analisis biometrik terkait kegiatan budidaya. Hasil penelitian menunjukkan bahwa nilai keragaman genetik populasi sintetis lebih tinggi 55,0%-287,5% dengan tingkat inbreeding 40,0%-77,14% lebih rendah dibanding populasi-populasi pembentuknya. Hal ini berdampak terhadap performa fenotipik populasi sintetis yang lebih baik, diindikasikan dengan peningkatan panjang, bobot akhir, dan tingkat produktivitas, masing-masing sebesar 2,5%-20,6%; 9,4%-61,8%; dan 18,2%-66,0% lebih baik dibanding populasi-populasi pembentuknya. Peningkatan kualitas genetik dan performa fenotipik populasi sintetis ini memberikan peluang untuk memperbaiki kualitas benih ikan mas pada kegiatan budidaya.Common carp in Indonesia has experienced a decline in genetic quality. The progressive decline leads to a significant decrease in carp performance in the farming environment. One of the efforts to genetically improve carp growth performance is through developing synthetic carp populations, which is a blend of the genetic potentials from several germplasm populations. This study aimed to form and evaluate the performance of genotypic and phenotypic of synthetic populations of common carp, blended from five strains of common carp, i.e., Rajadanu, Majalaya, Sutisna, Wildan, and Sinyonya. The genotypic performance was evaluated using the DNA microsatellite method. The phenotypic performance was assessed using biometric analysis, especially in terms of culture performance. The results showed that the genotypic performance of the synthetic populations of common carp was better than that of the founder strains. This performance was indicated by higher genetic diversity values, about 55.0%-287.5% and lower levels of inbreeding, about 40.0%-77.1%, compared with their founder populations. Phenotypic performance of the synthetic populations is also better than their founder populations, indicated by higher body length, weight, and productivity, about 2.5%-20.6%, 9.4%-61.8%, and 18.2%-66.0%, respectively. The improvement on genetic quality and phenotypic performance of the synthetic population provide opportunities to improve the quality of common carp fry in aquaculture activity.

Culture Performance and Economic Return of Brown Shrimp (Metapenaeus Monoceros) at Different Stocking Densities Reared in Brackishwater Pond

Shrimp recognized as the white gold of Bangladesh because it is the second largest export earning product after garments sector. The brown shrimp (M. monoceros) have high growth rates together with that they tolerate wide ranges of salinity and environmental parameters which makes them highly attractive for culture purposes. The purposes of this research were to assess the culture performance and economic profitability of brown shrimp (Metapenaeus monoceros) in brackish water ponds. This research lasted from February to June 2020 under three different stocking densities such as 35, 45 and 55 individuals/m2 in treatments T1, T2 and T3 at Bangladesh Fisheries Research Institute, Brackishwater Station, Paikgacha, Khulna. After 90 days culture periods the total production was 1703.32±144.48, 2768.25±167.63 and 2535.03±253.52 kg/ha in T1, T2 and T3 respectively which was significantly higher (p<0.05) in T2 compared to T1 and T2. Benefit cost ratio (BCR) was 0.32, 0.87 and 0.52 in T1, T2 and T3 respectively and found significantly higher (p<0.05) in T2 than T1and T3. Both culture performance and economic analysis implies that brown shrimp (M. monoceros) with a stocking density of 450000 individuals/ha might be environment conciliatory and economically enduring in coastal areas of Bangladesh.

Cultural Intelligence

For individuals in management positions, globalization has reinforced contact with foreign cultures and provided opportunities to be assigned to projects abroad. Expatriates encounter additional challenges varying from differently-oriented work forces to building a new life in a foreign country. The organizations must ensure that employees are well selected, prepared, and supported throughout the expatriation process. An exploratory study of German project managers in Portugal is conducted to elaborate the importance of cultural intelligence in business. Current understandings of both national cultures and their potential conflict factors are portrayed and German expatriates questioned about their work environment and private adjustment to Portuguese culture. Performance-orientation and assertive culture on the managers' side and relationship-focus and high-context communication on the employees' side triggered difficulties with work tasks. Differences related to the cultural dimension collectivism versus individualism complicated the expatriates' adjustment to the local culture.