The cytosolic pathway of L -malic acid synthesis in Saccharomyces cerevisiae : the role of fumarase

1996 ◽  
Vol 46 (4) ◽  
pp. 393-399
Author(s):  
O. Pines ◽  
S. Even-Ram ◽  
N. Elnathan ◽  
E. Battat ◽  
O. Aharonov ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Malic Acid ◽  
Acid Synthesis

The cytosolic pathway of l-malic acid synthesis in Saccharomyces cerevisiae: the role of fumarase

1996 ◽  
Vol 46 (4) ◽  
pp. 393-399 ◽  
Author(s):  
O. Pines ◽  
S. Even-Ram ◽  
N. Elnathan ◽  
E. Battat ◽  
O. Aharonov ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Malic Acid ◽  
Acid Synthesis

scholarly journals The role of malate in the synthesis of glutamate in Pisum arvense roots

2014 ◽  
Vol 55 (4) ◽  
pp. 611-619
Author(s):  
Genowefa Kubik-Dorosz
Keyword(s):  
Glutamic Acid ◽  
Malic Acid ◽  
Glutamate Synthase ◽  
Acid Synthesis ◽  
Incubation Mixture ◽  
Pyridine Nucleotides ◽  
Pisum Arvense

The in vivo and in vitro activities of NADH-dependent glutamate synthase in excised <em>Pisum arvense</em> roots increased several-fold under the influence of malate while pyruvate oxaloacctate. citrate and succinate inhibited this entyme. The plastids isolated from <em>Pisum arvense</em> root,. ahen incubated with glutamine and α-ketoglutarate, released glutamate into the medium Malate clearly stimulated this process. Albizziin (25 mM) completely reduced the presence of glutamate in the incubation mixture. These results indicate that reduced pyridine nucleotides arising in <em>P. arvense</em> root plastids during oxidation of malic acid may constitute the indispensable source of electrons for glutamic acid synthesis.

The Path of Carbon in CAM Plants at Night

1975 ◽  
Vol 2 (3) ◽  
pp. 377
Author(s):  
B.G Sutton
Keyword(s):  
Malic Acid ◽  
Tricarboxylic Acid Cycle ◽  
Acid Synthesis ◽  
Free Sugar ◽  
Cam Plants ◽  
Influence Of Temperature On ◽  
Quantitative Examination ◽  
Higher Temperature ◽  
Pass Through

Following demonstrations that malic acid synthesis in crassulacean acid metabolism (CAM) plants at night requires only the carboxylation of phosphoenolpyruvate, glycolysis has been presumed to supply this substrate. This study has examined the path of carbon in CAM plants at night to establish the involvement of intermediary pools of metabolites in other processes, and to define characteristics of the pathway which are likely to be involved in its regulation. In both Bryophyllum tubiflorum Barv. and Kalanchoe daigremontiana Hamet et Perrier, carbon loss from starch was insufficient to account for malic acid gains. Quantitative examination of the pool sizes of various carbohydrates and malic acid during the dark showed that starch was only part of a larger glucan pool which was the carbon source material for phosphoenolpyruvate supply. Observations of the utilization of 14C-labelled glucan in a CAM plant in the dark supported the role of this compound as primary carbon donor for acid synthesis. The mainstream of carbon flow was shown not to pass through the free sugar pools, and it was also proposed that malic and citric acids were able to exchange carbon via tricarboxylic acid cycle reactions. Examination of the influence of temperature on the glucan-malic acid relationship suggested that the depressed malic acid levels accumulated after nights at higher temperature were due to regulation of the reactions concerned with acid synthesis from glucan.

scholarly journals The manipulation of cellular cytochrome and lipid composition in a haem mutant of Saccharomyces cerevisiae

1977 ◽  
Vol 166 (2) ◽  
pp. 275-285 ◽  
Author(s):  
A M Astin ◽  
J M Haslam
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Protoporphyrin Ix ◽  
Total Sterol ◽  
Acid Synthesis ◽  
Progressive Increase ◽  
Sterol Synthesis ◽  
Early Lesion

1. The ole-3 mutant of Saccharomyces cerevisiae has an early lesion in the pathway of porphyrin biosynthesis. 2. This results in the loss of all haem-containing enzymes, including the mitochondrial cytochromes, and prevents the synthesis of components whose formation requires haem-containing enzymes, including unsaturated fatty acids, ergosterol and methionine. 3. The pleiotropic effects of the primary lesion are reversed by growing mutant ole-3 aerobically in the presence of intermediates of the porphyrin-biosynthetic pathway, and the present work reports the degree of manipulation of lipid and respiratory-cytochrome composition. 4. Supplements of delta-aminolaevulinate in the range 0.5–500 mg/l result in a progressive increase in the cellular content of unsaturated fatty acids and respiratory cytochromes, cause the replacement of lanosterol and squalene by ergosterol, and an increase in total sterol content. 5. Haematoporphyrin and protoporphyrin IX have similar but less extensive effects on cellular composition, whereas haematin allows unsaturated fatty acid synthesis and some sterol synthesis, but has no effect on the formation of respiratory cytochromes. 6. These results suggest that growth of the organism in the presence of defined amounts of delta-aminolaevulinate will be useful in the investigation of the role of lipids and cytochromes in the function and assembly of mitochondrial membranes.

The dual role of biotin in glucose metabolism of Saccharomyces cerevisiae

1955 ◽  
Vol 55 (2) ◽  
pp. 307-309 ◽  
Author(s):  
Herman C. Lichstein ◽  
Ruth B. Boyd
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Glucose Metabolism ◽  
Dual Role

Investigating the role of the transcriptional regulator Ure2 on the metabolism of Saccharomyces cerevisiae: a multi-omics approach

2021 ◽  
Author(s):  
Jing-Jing Liu ◽  
William Woodruff ◽  
Anshu Deewan ◽  
Sujit Sadashiv Jagtap ◽  
Eun Ju Yun ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transcriptional Regulator

Protective role of l ‐threonine against cadmium toxicity in Saccharomyces cerevisiae

2021 ◽  
Author(s):  
Linru Huang ◽  
Zhijia Fang ◽  
Jian Gao ◽  
Jingwen Wang ◽  
Yongbin Li ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cadmium Toxicity ◽  
Protective Role

scholarly journals The Role of Deoxyribonucleic Acid in Ribonucleic Acid Synthesis

1962 ◽  
Vol 237 (12) ◽  
pp. 3778-3785 ◽  
Author(s):  
Fred M. Kahan ◽  
Jerard Hurwitz
Keyword(s):  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Acid Synthesis
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