A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicum with xenogeneic plasmid DNA

1999 ◽  
Vol 52 (4) ◽  
pp. 541-545 ◽  
Author(s):  
M. E. van der Rest ◽  
C. Lange ◽  
D. Molenaar
Keyword(s):  
Heat Shock ◽  
Corynebacterium Glutamicum ◽  
Plasmid Dna ◽  
Efficient Transformation ◽  
Highly Efficient

Highly efficient biosynthesis of l-ornithine from mannitol by using recombinant Corynebacterium glutamicum

2021 ◽  
Vol 327 ◽  
pp. 124799
Author(s):  
Qi Sheng ◽  
Xiaoyu Wu ◽  
Yan Jiang ◽  
Zhimin Li ◽  
Fei Wang ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Highly Efficient

scholarly journals Highly efficient and tumor-selective nanoparticles for dual-targeted immunogene therapy against cancer

2020 ◽  
Vol 6 (3) ◽  
pp. eaax5032 ◽  
Author(s):  
Kuan-Wei Huang ◽  
Fu-Fei Hsu ◽  
Jiantai Timothy Qiu ◽  
Guann-Jen Chern ◽  
Yi-An Lee ◽  
...  
Keyword(s):  
Tumor Microenvironment ◽  
Cancer Immunotherapy ◽  
Plasmid Dna ◽  
Systemic Toxicity ◽  
Great Promise ◽  
Anticancer Efficacy ◽  
Highly Efficient ◽  
Immunogene Therapy ◽  
Immunosuppressive Tumor Microenvironment ◽  
Tumor Selective

While immunotherapy holds great promise for combating cancer, the limited efficacy due to an immunosuppressive tumor microenvironment and systemic toxicity hinder the broader application of cancer immunotherapy. Here, we report a combinatorial immunotherapy approach that uses a highly efficient and tumor-selective gene carrier to improve anticancer efficacy and circumvent the systemic toxicity. In this study, we engineered tumor-targeted lipid-dendrimer-calcium-phosphate (TT-LDCP) nanoparticles (NPs) with thymine-functionalized dendrimers that exhibit not only enhanced gene delivery capacity but also immune adjuvant properties by activating the stimulator of interferon genes (STING)–cGAS pathway. TT-LDCP NPs delivered siRNA against immune checkpoint ligand PD-L1 and immunostimulatory IL-2–encoding plasmid DNA to hepatocellular carcinoma (HCC), increased tumoral infiltration and activation of CD8+ T cells, augmented the efficacy of cancer vaccine immunotherapy, and suppressed HCC progression. Our work presents nanotechnology-enabled dual delivery of siRNA and plasmid DNA that selectively targets and reprograms the immunosuppressive tumor microenvironment to improve cancer immunotherapy.

Highly efficient transformation of linear poly(phenylene ethynylene)s into zigzag-shaped π-conjugated microporous polymers through boron-mediated alkyne benzannulation

2018 ◽  
Vol 2 (4) ◽  
pp. 807-814 ◽  
Author(s):  
Yoshiaki Shoji ◽  
Minsu Hwang ◽  
Haruka Sugiyama ◽  
Fumitaka Ishiwari ◽  
Kumiko Takenouchi ◽  
...  
Keyword(s):  
Phenylene Ethynylene ◽  
Microporous Polymers ◽  
Efficient Transformation ◽  
Highly Efficient ◽  
Linear Poly ◽  
Conjugated Microporous Polymers

Linear poly(phenylene ethynylene)s are directly converted into zigzag-shaped π-conjugated microporous polymers.

A novel electroporation procedure for highly efficient transformation of Lipomyces starkeyi

2020 ◽  
Vol 169 ◽  
pp. 105816 ◽  
Author(s):  
Hiroaki Takaku ◽  
Atsumi Miyajima ◽  
Haruka Kazama ◽  
Rikako Sato ◽  
Satoshi Ara ◽  
...  
Keyword(s):  
Lipomyces Starkeyi ◽  
Efficient Transformation ◽  
Highly Efficient

Novel episomal vectors and a highly efficient transformation procedure for the fission yeast Schizosaccharomyces japonicus

Yeast ◽  
2010 ◽  
Vol 27 (12) ◽  
pp. 1049-1060 ◽  
Author(s):  
Keita Aoki ◽  
Reiko Nakajima ◽  
Kanji Furuya ◽  
Hironori Niki
Keyword(s):  
Fission Yeast ◽  
Transformation Procedure ◽  
Efficient Transformation ◽  
Highly Efficient ◽  
Episomal Vectors

Highly Efficient Transformation of Bisphenol S by Anaerobic Cometabolism in the Bioelectrochemical System

2019 ◽  
Vol 145 (11) ◽  
pp. 04019079
Author(s):  
Jingyun Sun ◽  
Yuan Xu ◽  
Lijia Jiang ◽  
Mengjie Fan ◽  
Haitao Xu ◽  
...  
Keyword(s):  
Bioelectrochemical System ◽  
Bisphenol S ◽  
Efficient Transformation ◽  
Highly Efficient

scholarly journals Identification and Application of Plasmids Suitable for Transfer of Foreign DNA to Members of the Genus Gordonia

2003 ◽  
Vol 69 (8) ◽  
pp. 4971-4974 ◽  
Author(s):  
Matthias Arenskötter ◽  
Dirk Baumeister ◽  
Rainer Kalscheuer ◽  
Alexander Steinbüchel
Keyword(s):  
Heat Shock ◽  
Gene Transfer ◽  
Plasmid Dna ◽  
Dna Transfer ◽  
Autonomous Replication ◽  
Recombinant Strains ◽  
Foreign Dna ◽  
Gordonia Polyisoprenivorans

ABSTRACT Gene transfer systems for Gordonia polyisoprenivorans strains VH2 and Y2K based on electroporation and conjugation, respectively, were established. Several parameters were optimized, resulting in transformation efficiencies of >4 × 105 CFU/μg of plasmid DNA. In contrast to most previously described electroporation protocols, the highest efficiencies were obtained by applying a heat shock after the intrinsic electroporation. Under these conditions, transfer and autonomous replication of plasmid pNC9503 was also demonstrated to proceed in G. alkanivorans DSM44187, G. nitida DSM44499T, G. rubropertincta DSM43197T, G. rubropertincta DSM46038, and G. terrae DSM43249T. Conjugational plasmid DNA transfer to G. polyisoprenivorans resulted in transfer frequencies of up to 5 × 10−6 of the recipient cells. Recombinant strains capable of polyhydroxyalkanoate synthesis from alkanes were constructed.

Microarray studies reveal a ‘differential response’ to moderate or severe heat shock of the HrcA- and HspR-dependent systems in Corynebacterium glutamicum

Microbiology ◽  
2009 ◽  
Vol 155 (2) ◽  
pp. 359-372 ◽  
Author(s):  
Carlos Barreiro ◽  
Diana Nakunst ◽  
Andrea T. Hüser ◽  
Héctor D. de Paz ◽  
Jörn Kalinowski ◽  
...  
Keyword(s):  
Heat Shock ◽  
Corynebacterium Glutamicum ◽  
Inverted Repeat ◽  
Profile Analysis ◽  
Differential Response ◽  
Reverse Transcription Pcr ◽  
Genome Wide ◽  
Number Of Genes ◽  
Clusters Of Orthologous Groups ◽  
Microarray Studies

Genome-wide transcription profile analysis of the heat-shocked wild-type strain under moderate (40 °C) and severe heat stress (50 °C) revealed that a large number of genes are differentially expressed after heat shock. Of these, 358 genes were upregulated and 420 were downregulated in response to moderate heat shock (40 °C) in Corynebacterium glutamicum. Our results confirmed the HrcA/controlling inverted repeat of chaperone expression (CIRCE)-dependent and HspR/HspR-associated inverted repeat (HAIR)-dependent upregulation of chaperones following heat shock. Other genes, including clusters of orthologous groups (COG) related to macromolecule biosynthesis and several transcriptional regulators (COG class K), were upregulated, explaining the large number of genes affected by heat shock. Mutants having deletions in the hrcA or hspR regulators were constructed, which allowed the complete identification of the genes controlled by those systems. The up- or downregulation of several genes observed in the microarray experiments was validated by Northern blot analyses and quantitative (real-time) reverse-transcription PCR. These analyses showed a heat-shock intensity-dependent response (‘differential response’) in the HspR/HAIR system, in contrast to the non-differential response shown by the HrcA/CIRCE-regulated genes.

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