Highly efficient biosynthesis of l-ornithine from mannitol by using recombinant Corynebacterium glutamicum

2021 ◽  
Vol 327 ◽  
pp. 124799
Author(s):  
Qi Sheng ◽  
Xiaoyu Wu ◽  
Yan Jiang ◽  
Zhimin Li ◽  
Fei Wang ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Highly Efficient

scholarly journals Construction and application of a CRISPR/Cas9-assisted genomic editing system for Corynebacterium glutamicum

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chengzhen Yao ◽  
Xiaoqing Hu ◽  
Xiaoyuan Wang
Keyword(s):  
Corynebacterium Glutamicum ◽  
Cell Factory ◽  
Temperature Sensitive ◽  
Microbial Cell Factory ◽  
Time Saving ◽  
Editing Efficiency ◽  
Highly Efficient ◽  
Native Promoter ◽  
Insertion And Deletion ◽  
Genomic Editing

AbstractCorynebacterium glutamicum is widely used as microbial cell factory for various bioproducts, but its genomic editing efficiency needs to be improved. In this study, a highly efficient CRISPR/Cas9-assisted genomic editing system for C. glutamicum was constructed. This system mainly involves a plasmid and can be used for both gene insertion and deletion in the chromosome of C. glutamicum. The recombinant plasmid for the target gene containing all the editing elements, and first constructed it in E. coli, then purified and transformed it into C. glutamicum. This temperature-sensitive plasmid was cured at high temperature after the genomic editing was completed in C. glutamicum. Using this genetic editing system, the genetic editing efficiency in C. glutamicum ATCC 13032 could reach 95%. The whole work of editing could be done in 8–9 days and showed most time-saving compared to the reported. Using this system, the native promoter of gdhA1 in ATCC 13032 has been replaced with the strong promoter PtacM, and more than 10 genes in ATCC 13032 have been deleted. The results demonstrate that this CRISPR/Cas9-assisted system is highly efficient and very suitable for genome editing in C. glutamicum.

Highly efficient and selective photocatalytic CO2 to CO conversion in aqueous solution

2020 ◽  
Vol 56 (27) ◽  
pp. 3851-3854 ◽  
Author(s):  
Xiaomin Chai ◽  
Hai-Hua Huang ◽  
Huiping Liu ◽  
Zhuofeng Ke ◽  
Wen-Wen Yong ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Photocatalytic Performance ◽  
Aqueous Media ◽  
Highly Efficient ◽  
Co Conversion

A Co-based complex displayed the highest photocatalytic performance for CO2 to CO conversion in aqueous media.

Tuning the pore structures and photocatalytic properties of a 2D covalent organic framework with multi-branched photoactive moieties

Nanoscale ◽  
2020 ◽  
Vol 12 (30) ◽  
pp. 16136-16142
Author(s):  
Xuan Wang ◽  
Ming-Jie Dong ◽  
Chuan-De Wu
Keyword(s):  
Photocatalytic Properties ◽  
Effective Strategy ◽  
Covalent Organic Framework ◽  
Highly Efficient ◽  
Pore Structures ◽  
Local Connection ◽  
Organic Framework

An effective strategy to incorporate accessible metalloporphyrin photoactive sites into 2D COFs by establishing a 3D local connection for highly efficient photocatalysis was developed.

CarR, a MarR-family regulator from Corynebacterium glutamicum, modulated antibiotic and aromatic compound resistance

2019 ◽  
Vol 476 (21) ◽  
pp. 3141-3159 ◽  
Author(s):  
Meiru Si ◽  
Can Chen ◽  
Zengfan Wei ◽  
Zhijin Gong ◽  
GuiZhi Li ◽  
...  
Keyword(s):  
Stress Response ◽  
Ligand Binding ◽  
Corynebacterium Glutamicum ◽  
Conformational Changes ◽  
Cysteine Oxidation ◽  
Transcriptional Regulatory ◽  
Ligand Free ◽  
Redox Sensing

Abstract MarR (multiple antibiotic resistance regulator) proteins are a family of transcriptional regulators that is prevalent in Corynebacterium glutamicum. Understanding the physiological and biochemical function of MarR homologs in C. glutamicum has focused on cysteine oxidation-based redox-sensing and substrate metabolism-involving regulators. In this study, we characterized the stress-related ligand-binding functions of the C. glutamicum MarR-type regulator CarR (C. glutamicum antibiotic-responding regulator). We demonstrate that CarR negatively regulates the expression of the carR (ncgl2886)–uspA (ncgl2887) operon and the adjacent, oppositely oriented gene ncgl2885, encoding the hypothetical deacylase DecE. We also show that CarR directly activates transcription of the ncgl2882–ncgl2884 operon, encoding the peptidoglycan synthesis operon (PSO) located upstream of carR in the opposite orientation. The addition of stress-associated ligands such as penicillin and streptomycin induced carR, uspA, decE, and PSO expression in vivo, as well as attenuated binding of CarR to operator DNA in vitro. Importantly, stress response-induced up-regulation of carR, uspA, and PSO gene expression correlated with cell resistance to β-lactam antibiotics and aromatic compounds. Six highly conserved residues in CarR were found to strongly influence its ligand binding and transcriptional regulatory properties. Collectively, the results indicate that the ligand binding of CarR induces its dissociation from the carR–uspA promoter to derepress carR and uspA transcription. Ligand-free CarR also activates PSO expression, which in turn contributes to C. glutamicum stress resistance. The outcomes indicate that the stress response mechanism of CarR in C. glutamicum occurs via ligand-induced conformational changes to the protein, not via cysteine oxidation-based thiol modifications.

Design of Highly Efficient and Compact RF-DC Conversion Circuit at mW-class by LE-FDTD Method

2010 ◽  
Vol E93-C (8) ◽  
pp. 1323-1332 ◽  
Author(s):  
Tsunayuki YAMAMOTO ◽  
Kazuhiro FUJIMORI ◽  
Minoru SANAGI ◽  
Shigeji NOGI
Keyword(s):  
Fdtd Method ◽  
Highly Efficient

The Possibility for Development of Highly Efficient Silicon Solar Cells with Base p-i-n Structure

2008 ◽  
Vol 67 (7) ◽  
pp. 645-653
Author(s):  
L. P. Shuba ◽  
M. V. Kirichenko ◽  
V. R. Kopach ◽  
V. A. Antonova ◽  
A. M. Listratenko
Keyword(s):  
Solar Cells ◽  
Silicon Solar Cells ◽  
Highly Efficient
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