Effect of overexpression of Saccharomyces cerevisiae Pad1p on the resistance to phenylacrylic acids and lignocellulose hydrolysates under aerobic and oxygen-limited conditions

2001 ◽  
Vol 57 (1) ◽  
pp. 167-174 ◽  
Author(s):  
S. Larsson ◽  
N.-O. Nilvebrant ◽  
L. Jönsson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Lignocellulose Hydrolysates

scholarly journals Development of a Saccharomyces cerevisiae Strain with Enhanced Resistance to Phenolic Fermentation Inhibitors in Lignocellulose Hydrolysates by Heterologous Expression of Laccase

2001 ◽  
Vol 67 (3) ◽  
pp. 1163-1170 ◽  
Author(s):  
Simona Larsson ◽  
Pierre Cassland ◽  
Leif J. Jönsson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Mass ◽  
Raw Materials ◽  
White Rot ◽  
White Rot Fungus ◽  
Fermentation Inhibitors ◽  
Factors Affecting ◽  
Ethanol Formation ◽  
Active Laccase ◽  
Lignocellulose Hydrolysates

ABSTRACT To improve production of fuel ethanol from renewable raw materials, laccase from the white rot fungus Trametes versicolor was expressed under control of the PGK1 promoter inSaccharomyces cerevisiae to increase its resistance to phenolic inhibitors in lignocellulose hydrolysates. It was found that the laccase activity could be enhanced twofold by simultaneous overexpression of the homologous t-SNARE Sso2p. The factors affecting the level of active laccase obtained, besides the cultivation temperature, included pH and aeration. Laccase-expressing and Sso2p-overexpressing S. cerevisiae was cultivated in the presence of coniferyl aldehyde to examine resistance to lignocellulose-derived phenolic fermentation inhibitors. The laccase-producing transformant had the ability to convert coniferyl aldehyde at a faster rate than a control transformant not expressing laccase, which enabled faster growth and ethanol formation. The laccase-producing transformant was also able to ferment a dilute acid spruce hydrolysate at a faster rate than the control transformant. A decrease in the content of low-molecular-mass aromatic compounds, accompanied by an increase in the content of high-molecular-mass compounds, was observed during fermentation with the laccase-expressing strain, illustrating that laccase was active even at the very low levels of oxygen supplied. Our results demonstrate the importance of phenolic compounds as fermentation inhibitors and the advantage of using laccase-expressing yeast strains for producing ethanol from lignocellulose.

Anti-Saccharomyces cerevisiae Antibodies in Inflammatory Bowel Disease: a Family Study

2001 ◽  
Vol 36 (2) ◽  
pp. 196-201 ◽  
Author(s):  
F. Seibold ◽  
O. Stich ◽  
R. Hufnagl ◽  
S. Kamil ◽  
M. Scheurlen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Family Study ◽  
Inflammatory Bowel

Antioxidant evaluation of Helichrysum sp. and Santolina sp. extracts on Saccharomyces cerevisiae cell model

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
N Grčić ◽  
R Oliveira ◽  
ACP Dias
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Model ◽  
Antioxidant Evaluation

Immunreaktion auf Saccharomyces cerevisiae Antigene in Patienten mit Morbus Crohn

2007 ◽  
Vol 45 (08) ◽  
Author(s):  
S Schmechel ◽  
V Schachinger ◽  
F Seibold ◽  
C Tillack ◽  
T Ochsenkühn ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Morbus Crohn
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