Cloning of the cel 9A gene and characterization of its gene product from marine bacterium Pseudomonas sp. SK38

2001 ◽  
Vol 57 (1-2) ◽  
pp. 138-145 ◽  
Author(s):  
S. Ryu ◽  
S. Cho ◽  
S. Park ◽  
W. Lim ◽  
M. Kim ◽  
...  
Keyword(s):  
Gene Product ◽  
Marine Bacterium ◽  
Pseudomonas Sp ◽  
Its Gene

Cloning of poly(3-hydroxybutyrate) depolymerase from a marine bacterium, Alcaligenes faecalis AE122, and characterization of its gene product

1997 ◽  
Vol 1352 (1) ◽  
pp. 113-122 ◽  
Author(s):  
Keiko Kita ◽  
Shun-ichiro Mashiba ◽  
Masatoshi Nagita ◽  
Kaori Ishimaru ◽  
Kenji Okamoto ◽  
...  
Keyword(s):  
Gene Product ◽  
Marine Bacterium ◽  
Alcaligenes Faecalis ◽  
Its Gene

The glgB-encoded glycogen branching enzyme is essential for glycogen accumulation in Corynebacterium glutamicum

Microbiology ◽  
2011 ◽  
Vol 157 (11) ◽  
pp. 3243-3251 ◽  
Author(s):  
Gerd M. Seibold ◽  
Katrin J. Breitinger ◽  
Raoul Kempkes ◽  
Leonard Both ◽  
Matthias Krämer ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Gene Product ◽  
Glycogen Synthase ◽  
Exponential Growth Phase ◽  
Branching Enzyme ◽  
Glycogen Accumulation ◽  
Glycosidic Bonds ◽  
Its Gene ◽  
Glycogen Branching Enzyme

Corynebacterium glutamicum transiently accumulates glycogen as carbon capacitor during the early exponential growth phase in media containing carbohydrates. In some bacteria glycogen is synthesized by the consecutive action of ADP-glucose pyrophosphorylase (GlgC), glycogen synthase (GlgA) and glycogen branching enzyme (GlgB). GlgC and GlgA of C. glutamicum have been shown to be necessary for glycogen accumulation in this organism. However, although cg1381 has been annotated as the putative C. glutamicum glgB gene, cg1381 and its gene product have not been characterized and their role in transient glycogen accumulation has not yet been investigated. We show here that the cg1381 gene product of C. glutamicum catalyses the formation of α-1,6-glycosidic bonds in polysaccharides and thus represents a glycogen branching enzyme. RT-PCR experiments revealed glgB to be co-transcribed with glgE, probably encoding a maltosyltransferase. Promoter activity assays with the glgE promoter region revealed carbon-source-dependent expression of the glgEB operon. Characterization of the growth and glycogen content of glgB-deficient and glgB-overexpressing strains showed that the glycogen branching enzyme GlgB is essential for glycogen formation in C. glutamicum. Taken together these results suggest that an interplay of the enzymes GlgC, GlgA and GlgB is not essential for growth, but is required for synthesis of the transient carbon capacitor glycogen in C. glutamicum.

scholarly journals Cloning of the Novel Gene Encoding β-Agarase C from a Marine Bacterium, Vibrio sp. Strain PO-303, and Characterization of the Gene Product

2006 ◽  
Vol 72 (9) ◽  
pp. 6399-6401 ◽  
Author(s):  
Jinhua Dong ◽  
Shinnosuke Hashikawa ◽  
Takafumi Konishi ◽  
Yutaka Tamaru ◽  
Toshiyoshi Araki
Keyword(s):  
Amino Acid ◽  
Gene Product ◽  
Glycoside Hydrolase ◽  
Marine Bacterium ◽  
Glycoside Hydrolase Family ◽  
The Novel ◽  
Amino Acid Residues ◽  
Gene Encoding ◽  
Hydrolase Family

ABSTRACT The β-agarase C gene (agaC) of a marine bacterium, Vibrio sp. strain PO-303, consisted of 1,437 bp encoding 478 amino acid residues. β-Agarase C was identified as the first β-agarase that cannot hydrolyze neoagarooctaose and smaller neoagarooligosaccharides and was assigned to a novel glycoside hydrolase family.

scholarly journals Analysis of the Borrelia burgdorferi GeHo fla gene and antigenic characterization of its gene product.

1991 ◽  
Vol 173 (4) ◽  
pp. 1452-1459 ◽  
Author(s):  
G S Gassmann ◽  
E Jacobs ◽  
R Deutzmann ◽  
U B Göbel
Keyword(s):  
Borrelia Burgdorferi ◽  
Gene Product ◽  
Antigenic Characterization ◽  
Its Gene

Molecular cloning of the Lon protease gene fromThermus thermophilusHB8 and characterization of its gene product

1999 ◽  
Vol 266 (3) ◽  
pp. 811-819 ◽  
Author(s):  
Satoru Watanabe ◽  
Tomonari Muramatsu ◽  
Hiroko Ao ◽  
Yoshie Hirayama ◽  
Kenji Takahashi ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Gene Product ◽  
Lon Protease ◽  
Protease Gene ◽  
Its Gene

Characterization of the FGFR-3 Gene and Its Gene Product

1991 ◽  
Vol 638 (1 The Fibroblas) ◽  
pp. 400-402 ◽  
Author(s):  
KATHLEEN KEEGAN ◽  
DANIELE. JOHNSON ◽  
LEWIS T. WILLIAMS ◽  
MICHAEL J. HAYMAN
Keyword(s):  
Gene Product ◽  
Its Gene

Nucleotide sequence of a cDNA coding for rathck tyrosine kinase and characterization of its gene product

1994 ◽  
Vol 19 (2) ◽  
pp. 117-129 ◽  
Author(s):  
B S Vijaya Gouri ◽  
V Rema ◽  
S Kamatkar ◽  
Ghansityam Swarup
Keyword(s):  
Nucleotide Sequence ◽  
Tyrosine Kinase ◽  
Gene Product ◽  
Its Gene
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