The promoter of Milk vetch dwarf virus component 8 confers effective gene expression in both dicot and monocot plants

2005 ◽  
Vol 24 (3) ◽  
pp. 155-163 ◽  
Author(s):  
Naomi Shirasawa-Seo ◽  
Yoshitaka Sano ◽  
Shigeo Nakamura ◽  
Taka Murakami ◽  
Yoko Gotoh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dwarf Virus ◽  
Virus Component ◽  
Milk Vetch Dwarf Virus

scholarly journals Characteristics of the promoters derived from the single-stranded DNA components of Milk vetch dwarf virus in transgenic tobacco

2005 ◽  
Vol 86 (6) ◽  
pp. 1851-1860 ◽  
Author(s):  
Naomi Shirasawa-Seo ◽  
Yoshitaka Sano ◽  
Shigeo Nakamura ◽  
Taka Murakami ◽  
Shigemi Seo ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Cell Types ◽  
Gus Expression ◽  
Cauliflower Mosaic Virus ◽  
Dwarf Virus ◽  
Promoter Regions ◽  
Meristematic Tissue ◽  
Link Protein ◽  
Gus Reporter ◽  
Milk Vetch Dwarf Virus

Predicted promoter regions of Milk vetch dwarf virus (MDV) components (C1–C11) were isolated and fused with a β-glucuronidase (GUS) reporter gene and the characteristics of the promoters were examined. In transgenic tobacco calli, promoters of MDV C4 (encoding a cell-cycle link protein), C5 and C7 (both encoding unknown proteins), C6 (encoding a nuclear-shuttle protein) and C8 (encoding a movement protein) generated a stronger level of GUS expression than the Cauliflower mosaic virus 35S RNA promoter (P35S). In leaves of transgenic tobacco plants, the promoters of C5 and C8 conferred a level of GUS activity comparable to that of P35S. Histochemical GUS analysis showed that the promoters of C4–C9, the latter encoding a capsid protein, were active in phloem and meristematic tissue. The promoter of C8 was also active in mesophyll and cortex cell types. A low level of activity was found for the promoters of C11, which encodes a master replication-initiator protein (Rep), and C1, C2, C3 and C10, which encode additional Reps, in both transgenic tobacco calli and plants.

Milk vetch dwarf virus infection in the Solanaceae and Caricaceae families in Southeast Asia

2020 ◽  
Vol 69 (6) ◽  
pp. 1026-1033 ◽  
Author(s):  
Aamir Lal ◽  
Eui‐Joon Kil ◽  
Vo T. B. Thuy ◽  
Chairina Fadhila ◽  
Phuong T. Ho ◽  
...  
Keyword(s):  
Virus Infection ◽  
Southeast Asia ◽  
Dwarf Virus ◽  
Milk Vetch Dwarf Virus

scholarly journals Coordinate regulation of replication and virion sense gene expression in wheat dwarf virus.

1992 ◽  
Vol 4 (2) ◽  
pp. 213-223 ◽  
Author(s):  
J M Hofer ◽  
E L Dekker ◽  
H V Reynolds ◽  
C J Woolston ◽  
B S Cox ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dwarf Virus ◽  
Wheat Dwarf Virus ◽  
Coordinate Regulation ◽  
Virion Sense

Detection and Partial Characterization of Milk vetch dwarf virus Isolates from Faba Bean (Vicia faba L.) in Yunnan Province, China

2010 ◽  
Vol 158 (1) ◽  
pp. 35-39 ◽  
Author(s):  
Safaa G. Kumari ◽  
Brendan Rodoni ◽  
Heinrich-Josef Vetten ◽  
Mai Hlaing Loh ◽  
Angela Freeman ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Faba Bean ◽  
Yunnan Province ◽  
Dwarf Virus ◽  
Partial Characterization ◽  
Vicia Faba L ◽  
Milk Vetch Dwarf Virus ◽  
Virus Isolates

scholarly journals The Repression of Cell Wall- and Plastid-Related Genes and the Induction of Defense-Related Genes in Rice Plants Infected with Rice dwarf virus

2007 ◽  
Vol 20 (3) ◽  
pp. 247-254 ◽  
Author(s):  
Takumi Shimizu ◽  
Kouji Satoh ◽  
Shoshi Kikuchi ◽  
Toshihiro Omura
Keyword(s):  
Gene Expression ◽  
Cell Wall ◽  
Viral Infection ◽  
Cell Walls ◽  
Dwarf Virus ◽  
Rice Dwarf Virus ◽  
Stunted Growth ◽  
Rice Plants ◽  
Expression Of Genes ◽  
Stress Related Genes

An analysis, using microarrays, of gene expression in rice plants infected with Rice dwarf virus revealed significant decreases in levels of expression of genes that are involved in the formation of cell walls, reflecting the stunted growth of diseased plants. The expression of plastid-related genes also was suppressed, as anticipated from the white chlorotic appearance of infected leaves. By contrast, the expression of defense- and stress-related genes was enhanced after viral infection. These results suggest that virus-infected rice plants attempt to survive viral infection and replication by raising the levels of expression of defense- and stress-related genes while suppressing the expression of genes required for the elongation of cells and photosynthesis.

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