Selection of transgenic citrus plants based on glyphosate tolerance conferred by a citrus 5-enolpyruvylshikimate-3-phosphate synthase variant

2021 ◽  
Author(s):  
Benjamin A. Merritt ◽  
Xudong Zhang ◽  
Eric W. Triplett ◽  
Zhonglin Mou ◽  
Vladimir Orbović
Keyword(s):  
Glyphosate Tolerance ◽  
Transgenic Citrus ◽  
Selection Of ◽  
Phosphate Synthase

scholarly journals Plastid-expressed 5-enolpyruvylshikimate-3-phosphate synthase genes provide high level glyphosate tolerance in tobacco

2001 ◽  
Vol 25 (3) ◽  
pp. 261-270 ◽  
Author(s):  
Guang-Ning Ye ◽  
Peter T.J. Hajdukiewicz ◽  
Debra Broyles ◽  
Damian Rodriguez ◽  
Charles W. Xu ◽  
...  
Keyword(s):  
Glyphosate Tolerance ◽  
High Level ◽  
Phosphate Synthase

Enhancement of glyphosate tolerance in rice (Oryza sativa L.) through mutation induction in EPSPS (5-enolpyruvylshikimate-3-phosphate synthase)

Plant Gene ◽  
2020 ◽  
Vol 22 ◽  
pp. 100225 ◽  
Author(s):  
Sedigheh Nasr Ramzi ◽  
Mohammad Mehdi Sohani ◽  
Reza Shirzadian-Khorramabad ◽  
Jafar Asghari ◽  
Mehriar Amininasab
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Mutation Induction ◽  
Glyphosate Tolerance ◽  
Phosphate Synthase

scholarly journals Improvement of Glyphosate Resistance through Concurrent Mutations in Three Amino Acids of the Ochrobactrum 5-Enopyruvylshikimate-3-Phosphate Synthase

2011 ◽  
Vol 77 (23) ◽  
pp. 8409-8414 ◽  
Author(s):  
Yong-Sheng Tian ◽  
Jing Xu ◽  
Ai-Sheng Xiong ◽  
Wei Zhao ◽  
Xiao-Yan Fu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Three Dimensional ◽  
Glyphosate Resistance ◽  
Site Directed Mutagenesis ◽  
Dimensional Structure ◽  
Ochrobactrum Anthropi ◽  
Glyphosate Tolerance ◽  
Content Type ◽  
Important Region ◽  
Phosphate Synthase

ABSTRACTA mutant of 5-enopyruvylshikimate-3-phosphate synthase fromOchrobactrum anthropiwas identified after four rounds of DNA shuffling and screening. Its ability to restore the growth of the mutant ER2799 cell on an M9 minimal medium containing 300 mM glyphosate led to its identification. The mutant had mutations in seven amino acids: E145G, N163H, N267S, P318R, M377V, M425T, and P438L. Among these mutations, N267S, P318R, and M425T have never been previously reported as important residues for glyphosate resistance. However, in the present study they were found by site-directed mutagenesis to collectively contribute to the improvement of glyphosate tolerance. Kinetic analyses of these three mutants demonstrated that the effectiveness of these three individual amino acid alterations on glyphosate tolerance was in the order P318R > M425T > N267S. The results of the kinetic analyses combined with a three-dimensional structure modeling of the location of P318R and M425T demonstrate that the lower hemisphere's upper surface is possibly another important region for glyphosate resistance. Furthermore, the transgenicArabidopsiswas obtained to confirm the potential of the mutant in developing glyphosate-resistant crops.

scholarly journals A Novel 5-Enolpyruvylshikimate-3-Phosphate Synthase Shows High Glyphosate Tolerance in Escherichia coli and Tobacco Plants

PLoS ONE ◽  
2012 ◽  
Vol 7 (6) ◽  
pp. e38718 ◽  
Author(s):  
Gaoyi Cao ◽  
Yunjun Liu ◽  
Shengxue Zhang ◽  
Xuewen Yang ◽  
Rongrong Chen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Glyphosate Tolerance ◽  
Tobacco Plants ◽  
Phosphate Synthase

scholarly journals Structural Basis of Glyphosate Tolerance Resulting from Mutations of Pro101inEscherichia coli5-Enolpyruvylshikimate-3-phosphate Synthase

2007 ◽  
Vol 282 (45) ◽  
pp. 32949-32955 ◽  
Author(s):  
Martha L. Healy-Fried ◽  
Todd Funke ◽  
Melanie A. Priestman ◽  
Huijong Han ◽  
Ernst Schönbrunn
Keyword(s):  
Structural Basis ◽  
Glyphosate Tolerance ◽  
Phosphate Synthase
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