Biochemical Characterization of Traditional Varieties of Apricots (Prunus armeniaca L.) of the Campania Region, Southern Italy

Campania is the most important region of Italy in the apricot cultivation, present mostly in the Vesuvio area. At least to the best of our knowledge, no studies are reporting the biochemical characterization of the considerable number of traditional apricot varieties present on this territory, including the qualitative and quantitative profile of the polyphenols present. Our work evaluated the content of β-carotene, total phenolics, phenolic profiles, ascorbic acid and antioxidant activity of 23 traditional varieties of apricots of the Campania region. Principal component analysis (PCA) highlighted that, in the two main groups, the antioxidant activity was strongly affected by the content of ascorbic acid (−0.89), or slightly affected by the content of total polyphenols (−0.67), respect to the content of ascorbic acid (−0.55), never by β-carotene. Chlorogenic acid (up to 55.07 μg g−1) and catechin (up to 96.15 μg g−1) resulted the most abundant polyphenols recognized through the chromatographic analysis. PCA, extended to the polyphenol profile, confirmed the distribution of the varieties in two large groups, evidencing once again the hierarchical distance of four varieties (“Panzona”, “Paolona” “Baracca” and “Boccucia Eboli”) compared to the others.

The Status of the European Integration Process of the Western Balkan Countries

For many years, the priority of foreign policy determined by subsequent governments of the six Western Balkan countries, i.e., Albania, Bosnia and Herzegovina, Kosovo, Montenegro, North Macedonia, and Serbia has been their accession to the European Union. Yet, in recent years, this process has slowed down, and so it can be assumed that in the coming years there will be no further enlargement of the EU to include any of the Western Balkan countries. The following article is aimed at analysing the present status of European integration with regard to the aforementioned states, and discusses the causes of regression in this process which can be identified on the side of the non-EU Western Balkan states and the European Union itself. Their integration is also a key issue in the context of the increasingly stronger presence of non-EU players such as China, Russia, and Turkey, all competing with the European Union for influence in this important region. The study was based on discourse analysis (including the critical discourse analysis approach) and content analysis.

THE STATE OF RESEARCH OF THE HISTORY OF BELARUSIANS IN THE SOUTH OF UKRAINE IN THE REVOLUTIONARY ERA: AN OVERVIEW OF MODERN PUBLICATIONS

The article is devoted to the analysis of contemporary Ukrainian, Belarusian and Polish historiography of the history of Belarusians in the south of Ukraine and in Odesa and the district during the Ukrainian National-Democratic Revolution. The scientific relevance of the problem of research is associated with an insufficient level of its research in Ukrainian historiography. This aspect was chosen with taking into account the fact that during the Ukrainian National Democratic Revolution of 1917-1920 the territory of Southern Ukraine, mainly the city of Odesa and the district, due to economic ties and its political significance, became an important region for concentration of Belarusians, their trade activities, participation in political life and national state construction, etc. Of particular interest are the researches of M. Sсhavlinskiy, D. Mikhalyuk, O. Zubko. The researchers from Odesa are represented insufficiently. Researchers address such important problems as the creation of the Belarusian National Council in Odesa, the Belarusian National Commissariat and the Consulate, the activities of Belarusian national organizations. Most of the research examines the history of Belarusians in the south of Ukraine and the city of Odesa in the context of studying other problems. As a result of the study, it was found that in modern historiography – Ukrainian, Belarusian, Polish – some aspects of the history of Belarusians in the south of Ukraine, mainly in Odesa and the district, were covered for the period of 1917-1919.The researches are as special as and more general, in which events in the region are mentioned briefly in the context of a wider problem related mainly to the Belarusian national movement and the Belarusian state construction. As a rule, the investigators are limited only to mentions of personalities, or briefly characterize the Odesa period of their biographies. Further research requires the problem of interaction between the Belarusian ethnic group in the population of Odesa and in the south of Ukraine in the early XX century and Belarusian refugees during World War I, also Belarusian cultural and educational activities in the south mentioned in the research literature, etc.

The Donor-Dependent and Colon-Region-Dependent Metabolism of (+)-Catechin by Colonic Microbiota in the Simulator of the Human Intestinal Microbial Ecosystem

The intestinal absorption of dietary catechins is quite low, resulting in most of them being metabolized by gut microbiota in the colon. It has been hypothesized that microbiota-derived metabolites may be partly responsible for the association between catechin consumption and beneficial cardiometabolic effects. Given the profound differences in gut microbiota composition and microbial load between individuals and across different colon regions, this study examined how microbial (+)-catechin metabolite profiles differ between colon regions and individuals. Batch exploration of the interindividual variability in (+)-catechin microbial metabolism resulted in a stratification based on metabolic efficiency: from the 12 tested donor microbiota, we identified a fast- and a slow-converting microbiota that was subsequently inoculated to SHIME, a dynamic model of the human gut. Monitoring of microbial (+)-catechin metabolites from proximal and distal colon compartments with UHPLC-MS and UPLC-IMS-Q-TOF-MS revealed profound donor-dependent and colon-region-dependent metabolite profiles with 5-(3′,4′-dihydroxyphenyl)-γ-valerolactone being the largest contributor to differences between the fast- and slow-converting microbiota and the distal colon being a more important region for (+)-catechin metabolism than the proximal colon. Our findings may contribute to further understanding the role of the gut microbiota as a determinant of interindividual variation in pharmacokinetics upon (+)-catechin ingestion.

Emerging chikungunya virus variants at the E1-E1 inter-glycoprotein spike interface impact virus attachment and Inflammation.

Chikungunya virus (CHIKV) is a re-emerging arthropod-borne alphavirus and a serious threat to human health. Therefore, efforts toward elucidating how this virus causes disease and the molecular mechanisms underlying steps of the viral replication cycle are crucial. Using an in vivo transmission system that allows intra-host evolution, we identified an emerging CHIKV variant carrying a mutation in the E1 glycoprotein (V156A) in the serum of mice and saliva of mosquitoes. E1 V156A has since emerged in humans during an outbreak in Brazil, co-occurring with a second mutation, E1 K211T, suggesting an important role for these residues in CHIKV biology. Given the emergence of these variants, we hypothesized that they function to promote CHIKV infectivity and subsequent disease. Here, we show that E1 V156A and E1 K211T modulate virus attachment and fusion and impact binding to heparin, a homolog of heparan sulfate, a key entry factor on host cells. These variants also exhibit differential neutralization by anti-glycoprotein monoclonal antibodies, suggesting structural impacts on the particle that may be responsible for altered interactions at the host membrane. Finally, E1 V156A and E1 K211T exhibit increased titers in an adult arthritic mouse model and induce increased foot-swelling at the site of injection. Taken together, this work has revealed new roles for E1 where discrete regions of the glycoprotein are able to modulate cell attachment and swelling within the host. IMPORTANCE Alphaviruses represent a growing threat to human health worldwide. The re-emerging alphavirus chikungunya virus (CHIKV) has rapidly spread to new geographic regions in the last several decades, causing overwhelming outbreaks of disease, yet there are no approved vaccines or therapeutics. The CHIKV glycoproteins are key determinants of CHIKV adaptation and virulence. In this study, we identify and characterize the emerging E1 glycoprotein variants, V156A and K211T, that have since emerged in nature. We demonstrate that E1 V156A and K211T function in virus attachment to cells, a role that until now has been only attributed to specific residues of the CHIKV E2 glycoprotein. We also demonstrate E1 V156A and K211T to increase foot-swelling of the ipsilateral foot in mice infected with these variants. Observing that these variants and other pathogenic variants occur at the E1-E1 inter-spike interface, we highlight this structurally important region as critical for multiple steps during CHIKV infection. Together, these studies further defines the function of E1 in CHIKV infection and can inform the development of therapeutic or preventative strategies.

Karstic Groundwater Origin and Recharge Process in Zagros Region: Insights From Stable Isotopes (δ18O and Δ2H) Approach

Zagros is an important region with high quality and quantity karstic water resources in the Middle East. This region provides a dominant part of potable and agricultural water needs for its inhabitants as well as agricultural water needs for nearby regions. Therefore, studying karstic water resources in Zagros by accurate methods such as stable isotopes techniques is very important. In this investigation, hydrological characteristics of groundwater resources including groundwater origin, recharge rate and recharge elevation have been studied using stable isotopes (18O and 2H). The results show that stable isotopes signatures in groundwater resources show notable variations across Zagros and groundwater resource mainly plot on south and west Zagros meteoric water lines. In addition, recharge elevation and recharge rate in groundwater resources also show significant variations in Zagros. Finally, the stable isotopes signatures in precipitation and groundwater has been used to study the role of each dominant air mass (contribution percentage of precipitation events originate from each air mass) in groundwater resources recharge using Simmr package in R language. Overall, groundwater resources in Zagros is recharged by precipitation events originate from various air masses and they have various recharge rates and recharge elevations.

Ancient DNA from a 2,700-year-old goitered gazelle (Gazella subgutturosa) confirms gazelle hunting in Iron Age Central Asia

Central Asia has been an important region connecting the different parts of Eurasia throughout history and prehistory, with large states developing in this region during the Iron Age. Archaeogenomics is a powerful addition to the zooarchaeological toolkit for understanding the relation of these societies to animals. Here, we present the genetic identification of a goitered gazelle specimen (Gazella subgutturosa) at the site Gazimulla-Tepa, in modern-day Uzbekistan, confirming hunting of the species in the region during the Iron Age. The sample was directly radiocarbon dated to 2724-2439 calBP. A phylogenetic analysis of the mitochondrial genome places the individual into the modern variation of G. subgutturosa. Our data does represent both the first ancient DNA and the first nuclear DNA sequences of this species. The lack of genomic resources available for this gazelle and related species prevented us from performing a more in-depth analysis of the nuclear sequences generated. Therefore, we are making our sequence data available to the research community to facilitate other research of this nowadays threatened species which has been subject to human hunting for several millennia across its entire range on the Asian continent.

Developing of natural gas transportation of Central Asia and its geopolitical and geo-economic aspects

The gas transportation of Central Asian countries has been traditionally main part of their economic sector, because of geographical location and natural resources. Geographical and geopolitical Central Asia has significant commercial and political consequences across the region, and there gas production and export potential of the key Central Asia plays key role of gas transportation sector. Central Asian located in the centre of Eurasian continent and its transportation gas sector has very less implications in the global marketplace. Nowadays, geo-strategic role of this region is very low on global energy market. Central Asia is an important region of the Eurasian continent, touching Asia in the East and Europe in the West. The region is rich in oil, gas, gold, uranium ore and other underground minerals, and occupies an important geopolitical and strategic position, in which historically interests converged and intertwined diverse forces. This region surrounded by the giant powers Russia and China is still and strongly influenced by the unstable situation in the Middle East, Afghanistan, Pakistan and other adjacent regions. In addition, in the context of the expected increase in demand for energy resources in China, India and other Asian countries, reliable supplies of oil and natural gas from the Central Asian region contribute to the stabilization of the international energy market, in connection with which the importance of this region in terms of providing energy security.

An Insight of RuBisCO Evolution through a Multilevel Approach

RuBisCO is the most abundant enzyme on earth; it regulates the organic carbon cycle in the biosphere. Studying its structural evolution will help to develop new strategies of genetic improvement in order to increase food production and mitigate CO2 emissions. In the present work, we evaluate how the evolution of sequence and structure among isoforms I, II and III of RuBisCO defines their intrinsic flexibility and residue-residue interactions. To do this, we used a multilevel approach based on phylogenetic inferences, multiple sequence alignment, normal mode analysis, and molecular dynamics. Our results show that the three isoforms exhibit greater fluctuation in the loop between αB and βC, and also present a positive correlation with loop 6, an important region for enzymatic activity because it regulates RuBisCO conformational states. Likewise, an increase in the flexibility of the loop structure between αB and βC, as well as Lys330 (form II) and Lys322 (form III) of loop 6, is important to increase photosynthetic efficiency. Thus, the cross-correlation dynamics analysis showed changes in the direction of movement of the secondary structures in the three isoforms. Finally, key amino acid residues related to the flexibility of the RuBisCO structure were indicated, providing important information for its enzymatic engineering.

Perspectives and strategies of religious tourism for a sustainable development of the economy

"ABSTRACT. The purpose of this paper is to analyze the importance of religious tourism in sustainable development and to find effective strategies through marketing optics and methodology. The issue of religious tourism is a topical one and not without controversy. Romanian tourism is currently facing many problems, such as: reduced turnover, very frequent changes in the legal framework, very rigid labor market, a deficient infrastructure in quantity and quality, repeated changes in the behavior of economic agents and population, an unfavorable economic environment. To achieve this goal, qualitative research was conducted among tourism producers, intermediaries and tourists in the Neamţ region - one of the most important tourist areas in Romania and also an important region with religious and rural tourism destinations. The results showed that there is a special concern regarding religious tourism and could contribute to the local development of the area. Keywords: religion; religious tourism; development; sustainability; local resources JEL classification: M3, Z12 "