Species richness and generalists–specialists mosaicism of symbiodiniacean symbionts in corals from Hong Kong revealed by high-throughput ITS sequencing

Coral Reefs ◽  
2021 ◽  
Author(s):  
Osama S. Saad ◽  
Xin Lin ◽  
Tsz Yan Ng ◽  
Ling Li ◽  
Put Ang ◽  
...  
Forests ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 593
Author(s):  
Janet E. Nichol ◽  
Sawaid Abbas

Global trends predict a continuous increase in the proportion of forest occupied by plantations up to the end of the 21st century, while a dramatic loss of biodiversity is foreseen as a result of anthropogenic exploitation and climate change. This study compares the role and performance of plantation policies in Hong Kong, with natural regeneration of secondary forest, using detailed spatio-temporal data extracted from a previous study. The study extends over a 70-year period from 1945 to 2014 using aerial photographs and satellite images of five time periods to document spatio-temporal trends in plantation forestry and natural forest succession. Field data on species richness and woody biomass at different stages of forest succession are compared with available data from plantations in the same study area. Results indicate that plantation forests support relatively few native species in the understory, with much lower species richness than naturally regenerated forest, even after 6 to 7 decades. Time-sequential maps of habitat change show that natural forest succession from barren grassy hillsides, progressed at an annual rate of 7.8%, from only 0.2% of the landscape post WWII, to over 37% today. Plantation forestry on the other hand has been less successful, and has even acted as a barrier to natural forest regeneration, as mono-cultural plantations from the late 1960s to 1980s are still plantations today, whereas other similar areas have succeeded naturally to forest. The theory of plantations acting as a nurse crop for a woody native understory is not supported, as Pinus massoniana plantations, destroyed by two deadly nematodes during the 1970s, apparently had no woody understory, as they were seen to have reverted to grassland in 1989 and are still mainly grassland today.


2020 ◽  
Vol 58 (8) ◽  
Author(s):  
Jonathan Hon-Kwan Chen ◽  
Cyril Chik-Yan Yip ◽  
Jasper Fuk-Woo Chan ◽  
Rosana Wing-Shan Poon ◽  
Kelvin Kai-Wang To ◽  
...  

ABSTRACT In December 2019, the coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) was first reported in the Hubei province of China and later spread all over the world. There was an urgent need of a high-throughput molecular test for screening the COVID-19 patients in the community. The Luminex NxTAG CoV extended panel is a high-throughput FDA emergency use-authorized molecular diagnostic assay for SARS-CoV-2 detection. This system targets three genes (ORF1ab, N, and E genes) of SARS-CoV-2, the ORF1ab region of SARS-CoV, and the ORF5 region of MERS-CoV. In this study, we evaluated the diagnostic performance of this system with nasopharyngeal swab specimens of 214 suspected COVID-19 patients in Hong Kong. The results were compared with our routine COVID-19 reverse transcription-PCR (RT-PCR) protocol with a LightMix SarbecoV E-gene kit and an in-house RdRp/Hel RT-PCR assay. The NxTAG CoV extended panel demonstrated 97.8% sensitivity and 100% specificity to SARS-CoV-2 in nasopharyngeal specimens. On low-viral load specimens, the sensitivity of the NxTAG panel could still maintain at 85.71%. Strong agreement was observed between the NxTAG panel and the routine COVID-19 RT-PCR protocol (kappa value = 0.98). Overall, the E gene target of the NxTAG panel demonstrated the highest sensitivity among the three SARS-CoV-2 targets, while the N gene targets demonstrated the least. In conclusion, the NxTAG CoV extended panel is simple to use, and it has high diagnostic sensitivity and specificity to SARS-CoV-2 in nasopharyngeal specimens. We recommend this diagnostic system for high-throughput COVID-19 screening in the community.


Author(s):  
Brian Morton

In the years 1996 and 1997, the pattern of deposition of beached coral heads and pieces onto the shore of Telecom Bay within the Cape d'Aguilar Marine Reserve, Hong Kong suggested that typhoons were a significant natural perturbation. In August 1997, 808 pieces weighing 60,930 g were washed up following passage of Typhoon Victor. 1997 was also Hong Kong's wettest year on record and a survey of the living corals in the reserve in 1998 showed changes in a number of ecological parameters of species richness, composition and diversity but, most noticeably, that the formerly dominant Goniastrea aspera had been superseded by Platygyra sinensis. In 1998 and 1999, this was reflected in the changed proportions of these two beached corals. Dramatically lowered salinities in the bay during July and August 1997 may have effected this change in relative dominance. Following Typhoon Dan in October 1998, 342 pieces of corals weighing 75,600 g were collected. The 1996 and 1997 pattern seemed to be repeating itself. 1999 was a bad year for severe tropical storms and typhoons in Hong Kong, seven being reported upon. Yet, after each one only 12,490 g (Typhoon Leo), 3390 g (Typhoon Maggie), 3550 g (Severe Tropical Storm: no-name), 55 g (Typhoon Sam), 4500 g (Typhoon York and Typhoon Cam) and 3160 g (Typhoon Dan) were washed up. That is, the seven tropical depressions deposited about 27,640 g coral, approximately the same amount as only Typhoon Sally in 1996 (25,000 g) and Tropical Storm Penny and Typhoon Babs in 1998 (24,574 g) and less than half that of Typhoon Victor in 1997 (60,000 g). In July 1996, Cape d'Aguilar was declared a marine reserve, fishing banned in its 18 hectares of sea and ghost nets removed. The fishing ban seems to be halting the dislodgement of corals and they are thus not now being beached by typhoons.


2011 ◽  
Vol 225 (2) ◽  
pp. 426-431 ◽  
Author(s):  
Evan Kyzar ◽  
Siddharth Gaikwad ◽  
Andrew Roth ◽  
Jeremy Green ◽  
Mimi Pham ◽  
...  

ZooKeys ◽  
2016 ◽  
Vol 597 ◽  
pp. 3-26 ◽  
Author(s):  
Jesus Gomez-Zurita ◽  
Anabela Cardoso ◽  
Indiana Coronado ◽  
Gissela De la Cadena ◽  
José A. Jurado-Rivera ◽  
...  

2019 ◽  
Vol 20 ◽  
pp. 101249 ◽  
Author(s):  
Priyanka Khati ◽  
Anita Sharma ◽  
Parul Chaudhary ◽  
Ashish Kumar Singh ◽  
Saurabh Gangola ◽  
...  

1990 ◽  
Vol 6 (1) ◽  
pp. 91-101 ◽  
Author(s):  
Ping-Man So ◽  
David Dudgeon

ABSTRACTNecrophagous Diptera were sampled in a seasonal tropical mixed forest in Hong Kong from February 1985 to May 1986 using carrion-baited traps. Six families of flies were caught but only Calliphoridae, Muscidae and Sarcophagidae were considered. They made up a total of 14,669 flies of 21 species in the sample. Total species abundance followed Fisher's log-series distribution. The commonest five species were calliphorids, constituting 79.44% of the total combined catch. Temperature was apparently the main factor affecting seasonality of necrophagous Diptera in Hong Kong. Fluctuations in total abundance, species richness and H' followed seasonal changes in weekly mean air temperatures.


2018 ◽  
Vol 50 (3) ◽  
pp. 299-312 ◽  
Author(s):  
Steven D. LEAVITT ◽  
Paul M. KIRIKA ◽  
Guillermo AMO DE PAZ ◽  
Jen-Pan HUANG ◽  
Jae-Seoun HUR ◽  
...  

AbstractSpecies richness is not evenly distributed across the tree of life and a limited number of lineages comprise an extraordinarily large number of species. In lichen-forming fungi, only two genera are known to be ‘ultradiverse’ (>500 species), with the most diverse genus, Xanthoparmelia, consisting of c. 820 species. While Australia and South Africa are known as current centres of diversity for Xanthoparmelia, it is not well known when and where this massive diversity arose. To better understand the geographical and temporal context of diversification in this diverse genus, we sampled 191 Xanthoparmelia specimens representing c. 124 species/species-level lineages from populations worldwide. From these specimens, we generated a multi-locus sequence data set using Sanger and high-throughput sequencing to reconstruct evolutionary relationships in Xanthoparmelia, estimate divergence times and reconstruct biogeographical histories in a maximum likelihood and Bayesian framework. This study corroborated the phylogenetic placement of several morphologically or chemically diverse taxa within Xanthoparmelia, such as Almbornia, Chondropsis, Karoowia, Namakwa, Neofuscelia, Omphalodiella, Paraparmelia, Placoparmelia and Xanthomaculina, in addition to improved phylogenetic resolution and reconstruction of previously unsampled lineages within Xanthoparmelia. Our data indicate that Xanthoparmelia most likely originated in Africa during the early Miocene, coinciding with global aridification and development of open habitats. Reconstructed biogeographical histories of Xanthoparmelia reveal diversification restricted to continents with infrequent intercontinental exchange by long-distance dispersal. While likely mechanisms by which Xanthoparmelia obtained strikingly high levels of species richness in Australia and South Africa remain uncertain, this study provides a framework for ongoing research into diverse lineages of lichen-forming fungi. Finally, our study highlights a novel approach for generating locus-specific molecular sequence data sets from high throughput metagenomic reads.


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