A new record of Sebastes koreanus from China based on morphological characters and DNA barcoding

2015 ◽  
Vol 33 (3) ◽  
pp. 590-596 ◽  
Author(s):  
Yalu Fang ◽  
Yoshiaki Kai ◽  
Takashi Yanagimoto ◽  
Na Song ◽  
Tianxiang Gao
Keyword(s):  
Dna Barcoding ◽  
New Record ◽  
Morphological Characters ◽  
Sebastes Koreanus

scholarly journals First record of roving coral grouper Plectropomus pessuliferus (Fowler, 1904) from Saint Martin’s Island, Bangladesh based on Morphology and DNA Barcoding

2021 ◽  
Vol 32 (2) ◽  
pp. 293-301
Author(s):  
MD JAYEDUL ISLAM ◽  
SHARMIN AKTER ◽  
PROVAKOR SARKAR ◽  
MOHAMMAD RASHED ◽  
IREEN PARVIN ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Bay Of Bengal ◽  
New Record ◽  
First Record ◽  
Morphological Characters ◽  
Marine Waters ◽  
First Report ◽  
Saint Martin ◽  
Northern Bay Of Bengal

A new record of Plectropomus pessuliferus (Serranidae: Epinephelinae) wasdocumented based on morphological characters and DNA barcoding. The species was collectedduring a regular survey for making an inventory of reef associated fishes in Saint Martin`sIsland, Bangladesh. This is the first report of roving coral grouper from the marine waters ofBangladesh validated by morpho-meristic analysis and DNA barcoding. This is also the firstreport from the northern Bay of Bengal.

A new record of a flathead fish (Teleostei: Platycephalidae) from China based on morphological characters and DNA barcoding

2013 ◽  
Vol 31 (3) ◽  
pp. 617-624 ◽  
Author(s):  
Yan Qin ◽  
Na Song ◽  
Jianwei Zou ◽  
Zhaohui Zhang ◽  
Guangping Cheng ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
New Record ◽  
Morphological Characters

scholarly journals New Species of Soldier Fly—Sargus bipunctatus (Scopoli, 1763) (Diptera: Stratiomyidae), Recorded from a Human Corpse in Europe—A Case Report

Insects ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 302
Author(s):  
Marek Michalski ◽  
Piotr Gadawski ◽  
Joanna Klemm ◽  
Krzysztof Szpila
Keyword(s):  
Case Report ◽  
New Species ◽  
Dna Barcoding ◽  
Forensic Entomology ◽  
Morphological Characters ◽  
Lower Limbs ◽  
Black Soldier Fly ◽  
Hermetia Illucens ◽  
Practical Applications ◽  
Human Corpse

The only European Stratiomyidae species known for feeding on human corpses was the black soldier fly Hermetia illucens (Linnaeus, 1758). Analysis of fauna found on a human corpse, discovered in central Poland, revealed the presence of feeding larvae of another species from this family: the twin-spot centurion fly Sargus bipunctatus (Scopoli, 1763). The investigated corpse was in a stage of advanced decomposition. The larvae were mainly observed in the adipocere formed on the back and lower limbs of the corpse, and in the mixture of litter and lumps of adipocere located under the corpse. Adult specimens and larvae were identified based on morphological characters, and final identification was confirmed using DNA barcoding. Implementing a combination of morphological and molecular methods provided a reliable way for distinguishing the larvae of S. bipunctatus and H. illucens. The potential of S. bipunctatus for practical applications in forensic entomology is currently difficult to assess. Wide and reliable use of S. bipunctatus in the practice of forensic entomology requires further studies of the bionomy of this fly.

scholarly journals A DNA barcode reference library of the French Polynesian shore fishes

2019 ◽  
Author(s):  
Erwan Delrieu-Trottin ◽  
Jeffrey T. Williams ◽  
Diane Pitassy ◽  
Amy Driskell ◽  
Nicolas Hubert ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Biological Diversity ◽  
Dna Barcode ◽  
Reef Fishes ◽  
French Polynesia ◽  
Morphological Characters ◽  
Reference Library ◽  
Society Islands ◽  
Extensive Collection ◽  
I Gene

AbstractThe emergence of DNA barcoding and metabarcoding opened new ways to study biological diversity, however, the completion of DNA barcode libraries is fundamental for such approaches to succeed. This dataset is a DNA barcode reference library (fragment of Cytochrome Oxydase I gene) for 2,190 specimens representing at least 540 species of shore fishes collected over 10 years at 154 sites across the four volcanic archipelagos of French Polynesia; the Austral, Gambier, Marquesas and Society Islands, a 5,000,000 km2area. At present, 65% of the known shore fish species of these archipelagoes possess a DNA barcode associated with preserved, photographed, tissue sampled and cataloged specimens, and extensive collection locality data. This dataset represents one of the most comprehensive DNA barcoding efforts for a vertebrate fauna to date. Considering the challenges associated with the conservation of coral reef fishes and the difficulties of accurately identifying species using morphological characters, this publicly available library is expected to be helpful for both authorities and academics in various fields.

scholarly journals First Record of Mansonia dyari in the State of Morelos, Mexico, Based on Morphology and COI DNA Barcoding

2020 ◽  
Vol 36 (1) ◽  
pp. 33-36
Author(s):  
Luis F. Hernández-Guevara ◽  
Francisco J. Sánchez-Rámos ◽  
Rahuel J. Chan-Chable ◽  
Luis M. Hernández-Triana ◽  
Ma. Teresa Valdés-Perezgasga ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Dna Barcoding ◽  
Cytochrome C Oxidase ◽  
Mosquito Species ◽  
First Record ◽  
Morphological Characters ◽  
The State ◽  
Historical Records ◽  
Cytochrome C Oxidase I ◽  
Medical Importance

ABSTRACT Collections of mosquitoes were conducted for the surveillance of species of medical importance in the state of Morelos, Mexico, in June 2017. Species collected included Mansonia (Mansonia) dyari, which was identified using morphological characters and cytochrome c oxidase I DNA barcoding. Although 3 species of genus Mansonia have been previously reported in Mexico, this is the 1st confirmed record of Ma. dyari in Morelos State, where no Mansonia species had been recorded. Historical records of Ma. dyari and Ma. indubitans in Mexico were reviewed. Therefore, this record increases the number of mosquito species occurring in Morelos to 46. The specimens collected in this study were deposited in the Culicidae collection of the Universidad Autónoma Agraria Antonio Narro, Unidad Laguna.

scholarly journals DNA Barcoding: Mixed results for big-headed flies (Diptera: Pipunculidae)

Zootaxa ◽  
2007 ◽  
Vol 1423 (1) ◽  
pp. 1-26 ◽  
Author(s):  
JEFFREY H. SKEVINGTON ◽  
CHRISTIAN KEHLMAIER ◽  
GUNILLA STÅHLS
Keyword(s):  
Dna Barcoding ◽  
Sequence Data ◽  
Phylogenetic Signal ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
Base Pairs ◽  
Data Set ◽  
Taxonomic Decision ◽  
Morphological Species

Sequence data from 658 base pairs of mitochondrial cytochrome c oxidase I (cox1) were analysed for 28 described species of Pipunculidae (Diptera) in an effort to test the concept of DNA Barcoding on this family. Two recently revised but distantly related pipunculid lineages with presumed different evolutionary histories were used for the test (Clistoabdominalis Skevington, 2001 and Nephrocerus Zetterstedt, 1838). An effort was made to test the concept using sister taxa and morphologically similar sibling species swarms in these two genera. Morphological species concepts for Clistoabdominalis taxa were either supported by cox1 data or found to be too broad. Most of the discordance could be accounted for after reassessing morphological characters. In these cases, the molecular data were invaluable in assisting taxonomic decision-making. The radiation of Nearctic species of Nephrocerus could not be diagnosed using cox1. The ability of cox1 to recover phylogenetic signal was also tested on Clistoabdominalis. Morphological data for Clistoabdominalis were combined with the molecular data set. The pipunculid phylogeny from molecular data closely resembles the published phylogeny based on morphology. Partitioned Bremer support is used to localize areas of conflict between the datasets.

scholarly journals The Utility of DNA Barcoding for the Species Identification of Larval Fish in the Lower Ing River, Thailand

2020 ◽  
Vol 20 (9) ◽  
pp. 671-679
Author(s):  
Dutrudi Panprommin ◽  
Kanyanat Soontornprasit ◽  
Siriluck Tuncharoen ◽  
Niti Iamchuen
Keyword(s):  
Dna Barcoding ◽  
Species Identification ◽  
Larval Fish ◽  
Average Length ◽  
Conservation Status ◽  
Morphological Characters ◽  
Nucleotide Sequences ◽  
Coi Gene ◽  
Fishery Resource ◽  
Sustainable Fishery

The species identification of larval fish is very important for sustainable fishery resource management. However, identification based on morphological characters is very difficult, complex and error-prone. DNA barcoding with the sequence of cytochrome c oxidase I (COI) gene was used to identify larval fish species from 10 stations in the tributaries of the lower Ing River. One hundred and six samples were collected between May 2016 and April 2017. The average length of the COI nucleotide sequences was approximately 640 bp. A total of 99 nucleotide sequences were identified in 35 species, 31 genera, 19 families and 9 orders, with 97-100% identity with entries in both the GenBank and BOLD databases. The genetic distance within species ranged from 0.000 to 0.004. However, seven samples were identified at only the genus level because their sequences had not been reported in any databases. Based on IUCN conservation status, most species were classified as least concern (77.14%). Approximately 69.23% of all species were related to human uses in fisheries, aquaculture or aquariums, whereas 30.77% of species were not assessed. Trichopsis vittata (family Osphronemidae) (90%) had the most frequency of occurrence, followed by Oryzias minutillus (family Adrianichthyidae) (70%) and Trichopodus trichopterus (family Osphronemidae) (70%).

scholarly journals Validating Morphometrics with DNA Barcoding to Reliably Separate Three Cryptic Species of Bombus Cresson (Hymenoptera: Apidae)

Insects ◽  
2020 ◽  
Vol 11 (10) ◽  
pp. 669
Author(s):  
Joan Milam ◽  
Dennis E. Johnson ◽  
Jeremy C. Andersen ◽  
Aliza B. Fassler ◽  
Desiree L. Narango ◽  
...  
Keyword(s):  
Cryptic Species ◽  
Dna Barcoding ◽  
Morphological Characters ◽  
Sympatric Species ◽  
Bumble Bees ◽  
Hair Color ◽  
Width Ratio ◽  
Large Size ◽  
Morphometric Methods ◽  
The Status

Despite their large size and striking markings, the identification of bumble bees (Bombus spp.) is surprisingly difficult. This is particularly true for three North American sympatric species in the subgenus Pyrobombus that are often misidentified: B. sandersoni Franklin, B. vagans Smith B. perplexus Cresson. Traditionally, the identification of these cryptic species was based on observations of differences in hair coloration and pattern and qualitative comparisons of morphological characters including malar length. Unfortunately, these characteristics do not reliably separate these species. We present quantitative morphometric methods to separate these species based on the malar length to width ratio (MRL) and the ratios of the malar length to flagellar segments 1 (MR1) and 3 (MR3) for queens and workers, and validated our determinations based on DNA barcoding. All three measurements discriminated queens of B. sandersoni and B. vagans with 100% accuracy. For workers, we achieved 99% accuracy by combining both MR1 and MR3 measurements, and 100% accuracy differentiating workers using MRL. Moreover, measurements were highly repeatable within and among both experienced and inexperienced observers. Our results, validated by genetic evidence, demonstrate that malar measurements provide accurate identifications of B. vagans and B. sandersoni. There was considerable overlap in the measurements between B. perplexus and B. sandersoni. However, these species can usually be reliably separated by combining malar ratio measurements with other morphological features like hair color. The ability to identify bumble bees is key to monitoring the status and trends of their populations, and the methods we present here advance these efforts.

New species and record of xanthid crabs (Crustacean, Decapoda) from Taiwan

Zootaxa ◽  
2020 ◽  
Vol 4809 (3) ◽  
pp. 535-546
Author(s):  
PAN-WEN HSUEH
Keyword(s):  
New Species ◽  
New Record ◽  
Morphological Characters ◽  
Length Ratio ◽  
Colour Pattern ◽  
Width Ratio ◽  
The Third ◽  
New Record Species ◽  
Shallow Subtidal ◽  
De Man

A new species and a new record species of xanthid crabs collected from shallow subtidal hard bottoms of Taiwanese waters are described in the present study. Garthasia ludaoensis n. sp. represents the second species for the genus and can be readily distinguished from the type species, G. americana (Garth, 1939), by having: 1) smaller width to length ratio of the carapace; 2) larger width to length ratio of the third maxilliped; 3) smaller width to length ratio of merus of the third maxilliped; 4) no distinct longitudinal keel on surfaces of sternites 1 and 2; 5) smaller length to width ratio of merus of the last ambulatory leg; 6) smaller width to length ratio of pleonite 2 of the male abdomen; 7) pleonite 2 in semicircular-shaped; 8) a tuft of long setae near distal opening of G1; and 9) distal end of basal segment without upright protrusion of G2. This genus is reported for the first time from Taiwan, as well as from the West Pacific. The second xanthid crab specimen examined in the present study has live colour pattern of the carapace and morphological characters similar to that of Zosimus maculatus (de Man, 1887) described from Ambon, Indonesia (about 3.7° S). The present finding extends the northern distribution range of this species to near 22° N and represents a new record of this species for Taiwan. 

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