Strigolactone (GR24) Induced Salinity Tolerance in Sunflower (Helianthus annuus L.) by Ameliorating Morpho-Physiological and Biochemical Attributes Under In Vitro Conditions

Author(s):  
Hira Zulfiqar ◽  
Muhammad Shahbaz ◽  
Muhammad Ahsan ◽  
Muhammad Nafees ◽  
Hammad Nadeem ◽  
...  
Helia ◽  
2004 ◽  
Vol 27 (40) ◽  
pp. 227-236 ◽  
Author(s):  
H. Turhan ◽  
I. Baser

Author(s):  
Ami Tjitraresmi ◽  
Moelyono Moektiwardoyo ◽  
Yasmiwar Susilawati

Malaria is a disease that occurs in tropical countries like Indonesia. The incidence of malaria in the world is still quite high and the occurrence of cases of Plasmodium resistance to antimalarial drugs and the widespread of resistance have prompted researchers to look for new antimalarial drugs, especially from natural materials. Betel leaf (Piper betle Linn.) And sunflower leaf (Helianthus annuus L.) have long been used by the people of Indonesia as an antimalarial drug. The purpose of this study was to determine antimalarial activity through inhibition of heme polymerization and determine secondary metabolite compounds by phytochemical screening from betel leaves and sunflower leaves. The heme polymerization inhibition activity assay was carried out by the in-vitro method using a microplate reader at 415 nm and 630 nm wavelengths. IC50 values of betel leaf extract and sunflower leaf were 178.67 μg/ml and 160.10 μg/ml, respectively. Phytochemical screening results from betel leaf showed the presence of flavonoids, polyphenols, tannins, quinones, saponins, and monoterpenoids-sesquiterpenoids, while sunflower leaves contain alkaloids, polyphenols, flavonoids, steroids and monoterpenoids-sesquiterpenoids.Keywords: Piper betle Linn., Helianthus annuus L., Malaria, Heme Polymerization


Author(s):  
Varsha V. Sonkamble ◽  
Nilesh S. Wagh ◽  
Laxmikant H. Kamble

Objective: This investigation includes characterization of phytochemicals from acetone extract of Helianthus annuus L. seeds responsible for α-amylase and α-glucosidase inhibition revealed from in vitro and in silico approaches.Methods: Seed extract was qualitatively and quantitatively analysed for the presence of bioactive molecules. In vitro α-amylase and α-glucosidase inhibition assays and kinetics studies for α-glucosidase were done. Thin layer chromatography (TLC) autography of extract was done to screen potent inhibitors and characterized by high-resolution liquid chromatography-mass spectrometry (HR LC-MS). Characterized molecules were further used for in silico studies.Results: Qualitative investigation reveals the presence of flavonoids, glycosides, alkaloids, terpenoids, and steroids. Quantitative analysis for total phenolic content and total flavonoid content of the extract was 0.1±0.005 mg/ml GAE and 0.025±0.003 mg/ml QE respectively. Percent inhibition of α-amylase and α-glucosidase ascertained in presence of extract was 60.42±0.6 and 83.22±0.18 at 0.01 mg while 36.24±0.81 and 37.67±0.15 at 0.005 mg of extracts for both enzymes respectively. Kinetics studies of α-glucosidase inhibition illustrated the non-competitive type of inhibition. TLC autography inhibition patterns were characterized by HR LC-MS. Characterized molecules on docking revealed (6RS)-22-hydroxy-23,24,25,26,27-pentanor-vitamin-D3-6,19-sulfurdioxide-adduct, manoalide and 5β-cholestane-3α,7α,12α,24,25,26-hexol as the best docked molecules with lowest binding energies of-12.5,-11 and-10.2 kcal/mol for α-amylase and-14.2,-11 and-11.2 kcal/mol for α-glucosidase respectively.Conclusion: Results clearly suggested that (6RS)-22-hydroxy-23,24,25,26,27-pentanor-vitamin-D3-6,19-sulfurdioxide-adduct, manoalide and 5β-cholestane-3α,7α,12α,24,25,26-hexol could be considered as lead molecules for the discovery of potent antidiabetic agents. 


2012 ◽  
Vol 15 (3) ◽  
pp. 205-217 ◽  
Author(s):  
Saeed Rauf ◽  
Muhammad Shahzad ◽  
Jaime A. Teixeira da Silva ◽  
Ijaz Rasool Noorka

2017 ◽  
Vol 110 ◽  
pp. 50-58 ◽  
Author(s):  
E. Tassi ◽  
L. Giorgetti ◽  
E. Morelli ◽  
J.R. Peralta-Videa ◽  
J.L. Gardea-Torresdey ◽  
...  

2015 ◽  
Vol 6 (3) ◽  
pp. 315 ◽  
Author(s):  
Ramón Gonzalo Aragadvay-Yungán ◽  
Adolfo Armando Rayas Amor ◽  
Darwin Heredia-Nava ◽  
Julieta Gertrudis Estrada-Flores ◽  
Francisco Ernesto Martínez-Castañeda ◽  
...  

El ensilado de girasol forrajero (Helianthus annuus L.) es promovido para la alimentación del ganado lechero ante escasez de riego y lluvias erráticas. El objetivo fue valorar al ensilado de girasol, solo y en diferentes proporciones con ensilado de maíz mediante degradación in vitro y la cinética de producción de gas. Se realizaron micro-silos, abiertos a los 80 días para el ensilado de maíz (EMz) y 50 días para el ensilado de girasol (EG), evaluándose los siguientes tratamientos (EMz%: EG%); T1) 100:0, T2) 75:25, T3) 50:50, T4) 25:75 y T5) 0:100. En el T2 la fibra detergente neutra y ácida disminuyeron significativamente (P<0.05), resultando en una digestibilidad in vitro de materia seca, materia orgánica, fibra detergente neutra y contenido de energía metabolizable y proteína cruda (P>0.05) similar a T1. Hubo mayor producción de gas total en T1, pero con una menor tasa de fermentación (P<0.05) al compararlo con T2 cuya tasa de fermentación fue de 0.0530/h, con un tiempo Lag de 3.4 h. El girasol aporta 31.8 % más de PC, 11.8 % menos de energía metabolizable y 11.9 % menos de digestibilidad in vitro. Comparado con el T1, el T2 aportó 1.6 % más de proteína, 2 % menos de energía metabolizable y 4.4 % menos de digestibilidad in vitro. Se concluye que el ensilado de girasol podría ser una alternativa para sustituir al ensilado de maíz hasta un 25 %, ya que el aporte de proteína y energía es similar al ensilado de maíz solo.


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