scholarly journals In vitro and in vivo water stress in sunflower, Helianthus annuus L

Helia ◽  
2004 ◽  
Vol 27 (40) ◽  
pp. 227-236 ◽  
Author(s):  
H. Turhan ◽  
I. Baser
Keyword(s):  
Water Stress ◽  
Helianthus Annuus ◽  
Helianthus Annuus L

INFLUENCE OF WATER STRESS, PHOSPHORUS AND ZINC APPLICATIONS ON, SEED QUALITY AND SEED YIELD OF SUNFLOWER (Helianthus annuus L.)

2012 ◽  
Vol 3 (4) ◽  
pp. 483-494
Author(s):  
S. A. Hammad ◽  
A. Y. Negm ◽  
A. M. El-Ghamry ◽  
M. A. Abou seada ◽  
Z. A. Lashin
Keyword(s):  
Water Stress ◽  
Helianthus Annuus ◽  
Seed Yield ◽  
Seed Quality ◽  
Helianthus Annuus L ◽  
Influence Of Water

scholarly journals Inhibition of Heme Polymerization Invitro Assay Of Extract of Sirih Leaf (Piper betle Linn.) and Sun Flower Leaves (Helianthus annuus L.)

2020 ◽  
Vol 7 (1) ◽  
pp. 22 ◽  
Author(s):  
Ami Tjitraresmi ◽  
Moelyono Moektiwardoyo ◽  
Yasmiwar Susilawati
Keyword(s):  
Helianthus Annuus ◽  
Antimalarial Activity ◽  
Antimalarial Drugs ◽  
Piper Betle ◽  
Phytochemical Screening ◽  
Helianthus Annuus L ◽  
Betel Leaf ◽  
The People ◽  
Ic50 Values

Malaria is a disease that occurs in tropical countries like Indonesia. The incidence of malaria in the world is still quite high and the occurrence of cases of Plasmodium resistance to antimalarial drugs and the widespread of resistance have prompted researchers to look for new antimalarial drugs, especially from natural materials. Betel leaf (Piper betle Linn.) And sunflower leaf (Helianthus annuus L.) have long been used by the people of Indonesia as an antimalarial drug. The purpose of this study was to determine antimalarial activity through inhibition of heme polymerization and determine secondary metabolite compounds by phytochemical screening from betel leaves and sunflower leaves. The heme polymerization inhibition activity assay was carried out by the in-vitro method using a microplate reader at 415 nm and 630 nm wavelengths. IC50 values of betel leaf extract and sunflower leaf were 178.67 μg/ml and 160.10 μg/ml, respectively. Phytochemical screening results from betel leaf showed the presence of flavonoids, polyphenols, tannins, quinones, saponins, and monoterpenoids-sesquiterpenoids, while sunflower leaves contain alkaloids, polyphenols, flavonoids, steroids and monoterpenoids-sesquiterpenoids.Keywords: Piper betle Linn., Helianthus annuus L., Malaria, Heme Polymerization

scholarly journals INHIBITION OF α-AMYLASE AND α-GLUCOSIDASE BY (6RS)-22-HYDROXY-23,24,25,26,27-PENTANOR-VITAMIN-D3-6,19-SULFUR DIOXIDE-ADDUCT, MANOALIDE AND 5β-CHOLESTANE-3α,7α,12α,24,25,26-HEXOL ISOLATED FROM ACETONE EXTRACT OF HELIANTHUS ANNUUS L. SEEDS

2018 ◽  
Vol 10 (5) ◽  
pp. 39 ◽  
Author(s):  
Varsha V. Sonkamble ◽  
Nilesh S. Wagh ◽  
Laxmikant H. Kamble
Keyword(s):  
Helianthus Annuus ◽  
Vitamin D3 ◽  
In Silico ◽  
Binding Energies ◽  
Acetone Extract ◽  
Bioactive Molecules ◽  
Total Phenolic ◽  
Helianthus Annuus L ◽  
Kinetics Studies

Objective: This investigation includes characterization of phytochemicals from acetone extract of Helianthus annuus L. seeds responsible for α-amylase and α-glucosidase inhibition revealed from in vitro and in silico approaches.Methods: Seed extract was qualitatively and quantitatively analysed for the presence of bioactive molecules. In vitro α-amylase and α-glucosidase inhibition assays and kinetics studies for α-glucosidase were done. Thin layer chromatography (TLC) autography of extract was done to screen potent inhibitors and characterized by high-resolution liquid chromatography-mass spectrometry (HR LC-MS). Characterized molecules were further used for in silico studies.Results: Qualitative investigation reveals the presence of flavonoids, glycosides, alkaloids, terpenoids, and steroids. Quantitative analysis for total phenolic content and total flavonoid content of the extract was 0.1±0.005 mg/ml GAE and 0.025±0.003 mg/ml QE respectively. Percent inhibition of α-amylase and α-glucosidase ascertained in presence of extract was 60.42±0.6 and 83.22±0.18 at 0.01 mg while 36.24±0.81 and 37.67±0.15 at 0.005 mg of extracts for both enzymes respectively. Kinetics studies of α-glucosidase inhibition illustrated the non-competitive type of inhibition. TLC autography inhibition patterns were characterized by HR LC-MS. Characterized molecules on docking revealed (6RS)-22-hydroxy-23,24,25,26,27-pentanor-vitamin-D3-6,19-sulfurdioxide-adduct, manoalide and 5β-cholestane-3α,7α,12α,24,25,26-hexol as the best docked molecules with lowest binding energies of-12.5,-11 and-10.2 kcal/mol for α-amylase and-14.2,-11 and-11.2 kcal/mol for α-glucosidase respectively.Conclusion: Results clearly suggested that (6RS)-22-hydroxy-23,24,25,26,27-pentanor-vitamin-D3-6,19-sulfurdioxide-adduct, manoalide and 5β-cholestane-3α,7α,12α,24,25,26-hexol could be considered as lead molecules for the discovery of potent antidiabetic agents. 

scholarly journals Recombinant Water Stress Protein 1 (Re-WSP1) Suppresses Colon Cancer Cell Growth Through Mir-539/Β-Catenin Signaling Pathway

2021 ◽  
Author(s):  
Songjia Guo ◽  
Shuhua Shan ◽  
Haili Wu ◽  
huiqiang hao ◽  
Zhuoyu Li
Keyword(s):  
Colon Cancer ◽  
Water Stress ◽  
Cell Growth ◽  
Molecular Mechanisms ◽  
Stress Protein ◽  
Pcr Analysis ◽  
Dependent Manner ◽  
Nostoc Commune

Abstract Nostoc commune Vauch is a nitrogen-fixing blue-green algae, contains a large number of active molecules with medicinal functions. Our previous study found that a water stress protein (WSP1) from Nostoc commune Vauch and its the recombinant protein (Re-WSP1) exhibited significant anti-colon cancer (CRC) activity both in vitro and in vivo. However, the underlying mechanism remains unknown. In this study, the CCK8 and clonogenic assays showed that Re-WSP1 restrained the colon cancer growth in a dose-dependent manner. Mechanistically, Re-WSP1 inhibited the expression of β-catenin, which was partly reversed by LiCl treatment, demonstrating a key role in Re-WSP1-induced inhibition of cell growth. Quantitative PCR analysis showed that the expression of microRNA-539 (miR-539) was significantly up-regulated upon Re-WSP1 treatment. Moreover, miR-539 negatively regulateed the expression of β-catenin through directly binds to the 3’UTR of β-catenin mRNA. Taken together, our data demonstrate that Re-WSP1 suppresses the CRC growth via miR-539/β-catenin axis, which provides new insights into the molecular mechanisms underlying Re-WSP1 against CRC.

scholarly journals Evaluación in vitro del ensilaje de girasol (Helianthus annuus L.) solo y combinado con ensilaje de maíz

2015 ◽  
Vol 6 (3) ◽  
pp. 315 ◽  
Author(s):  
Ramón Gonzalo Aragadvay-Yungán ◽  
Adolfo Armando Rayas Amor ◽  
Darwin Heredia-Nava ◽  
Julieta Gertrudis Estrada-Flores ◽  
Francisco Ernesto Martínez-Castañeda ◽  
...  
Keyword(s):  
Helianthus Annuus ◽  
Helianthus Annuus L

El ensilado de girasol forrajero (Helianthus annuus L.) es promovido para la alimentación del ganado lechero ante escasez de riego y lluvias erráticas. El objetivo fue valorar al ensilado de girasol, solo y en diferentes proporciones con ensilado de maíz mediante degradación in vitro y la cinética de producción de gas. Se realizaron micro-silos, abiertos a los 80 días para el ensilado de maíz (EMz) y 50 días para el ensilado de girasol (EG), evaluándose los siguientes tratamientos (EMz%: EG%); T1) 100:0, T2) 75:25, T3) 50:50, T4) 25:75 y T5) 0:100. En el T2 la fibra detergente neutra y ácida disminuyeron significativamente (P<0.05), resultando en una digestibilidad in vitro de materia seca, materia orgánica, fibra detergente neutra y contenido de energía metabolizable y proteína cruda (P>0.05) similar a T1. Hubo mayor producción de gas total en T1, pero con una menor tasa de fermentación (P<0.05) al compararlo con T2 cuya tasa de fermentación fue de 0.0530/h, con un tiempo Lag de 3.4 h. El girasol aporta 31.8 % más de PC, 11.8 % menos de energía metabolizable y 11.9 % menos de digestibilidad in vitro. Comparado con el T1, el T2 aportó 1.6 % más de proteína, 2 % menos de energía metabolizable y 4.4 % menos de digestibilidad in vitro. Se concluye que el ensilado de girasol podría ser una alternativa para sustituir al ensilado de maíz hasta un 25 %, ya que el aporte de proteína y energía es similar al ensilado de maíz solo.

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