Cardiorespiratory reflexes and aquatic surface respiration in the neotropical fish tambaqui ( Colossoma macropomum ): acute responses to hypercarbia

This study examined the location and physiological roles of branchial chemoreceptors involved in the cardiorespiratory responses to hypoxia and hypercarbia in a neotropical fish that exhibits aquatic surface respiration, the tambaqui (Colossoma macropomum). Fish were exposed to abrupt progressive environmental hypoxia (18. 6–1.3 kPa water P(O2)) and hypercarbia (water equilibrated with 5 % CO(2) in air, which lowered the water pH from 7.0 to 5.0). They were also subjected to injections of NaCN into the ventral aorta (to stimulate receptors monitoring the blood) and buccal cavity (to stimulate receptors monitoring the respiratory water). All tests were performed before and after selective denervation of branchial branches of cranial nerves IX and X to the gill arches. The data suggest that the O(2) receptors eliciting reflex bradycardia and increases in breathing frequency are situated on all gill arches and sense changes in both the blood and respiratory water and that the O(2) receptors triggering the elevation in systemic vascular resistance, breathing amplitude, swelling of the inferior lip and that induce aquatic surface respiration during hypoxia are extrabranchial, although branchial receptors also contribute to the latter two responses. Hypercarbia also produced bradycardia and increases in breathing frequency, as well as hypertension, and, while the data suggest that there may be receptors uniquely sensitive to changes in CO(2)/pH involved in cardiorespiratory control, this is based on quantitative rather than qualitative differences in receptor responses. These data reveal yet another novel combination for the distribution of cardiorespiratory chemoreceptors in fish from which teleologically satisfying trends have yet to emerge.

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The role of branchial and orobranchial O2 chemoreceptors in the control of aquatic surface respiration in the neotropical fish tambaqui (Colossoma macropomum): progressive responses to prolonged hypoxia

Journal of Experimental Biology ◽

10.1242/jeb.02199 ◽

2006 ◽

Vol 209 (9) ◽

pp. 1709-1715 ◽

Cited By ~ 33

Author(s):

L. H. Florindo

Keyword(s):

Aquatic Surface Respiration ◽

Neotropical Fish ◽

Colossoma Macropomum

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Histopathological changes in gill, liver and kidney of neotropical fish Colossoma macropomum exposed to paraquat at different temperatures

Environmental Toxicology and Pharmacology ◽

10.1016/j.etap.2011.02.002 ◽

2011 ◽

Vol 31 (3) ◽

pp. 490-495 ◽

Cited By ~ 18

Author(s):

Raquel Salazar-Lugo ◽

Claunis Mata ◽

Aridays Oliveros ◽

Luz Marina Rojas ◽

Mairin Lemus ◽

...

Keyword(s):

Histopathological Changes ◽

Neotropical Fish ◽

Colossoma Macropomum ◽

Different Temperatures ◽

Liver And Kidney

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SNPs markers for the heavily overfished tambaqui Colossoma macropomum, a Neotropical fish, using next-generation sequencing-based de novo genotyping

Conservation Genetics Resources ◽

10.1007/s12686-016-0610-3 ◽

2016 ◽

Vol 9 (1) ◽

pp. 29-33 ◽

Cited By ~ 4

Author(s):

José Gregorio Martínez ◽

Valéria Nogueira Machado ◽

Susana J. Caballero-Gaitán ◽

Maria da C. Freitas Santos ◽

Rodrigo Maciel Alencar ◽

...

Keyword(s):

Next Generation Sequencing ◽

De Novo ◽

Next Generation ◽

Neotropical Fish ◽

Colossoma Macropomum ◽

Generation Sequencing

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Effect of chronic cadmium exposure on structure of head kidney of neotropical fish Colossoma macropomum

Toxicology Letters ◽

10.1016/j.toxlet.2011.05.480 ◽

2011 ◽

Vol 205 ◽

pp. S135

Author(s):

R.D. Salazar-Lugo ◽

A.B. Vargas ◽

L.M. Rojas

Keyword(s):

Head Kidney ◽

Cadmium Exposure ◽

Neotropical Fish ◽

Colossoma Macropomum

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Transferability of heterologous microsatellite primers in Brycon gouldingi

Ciência Rural ◽

10.1590/0103-8478cr20180412 ◽

2018 ◽

Vol 48 (11) ◽

Author(s):

Felipe Pinheiro de Souza ◽

Ed Christian Suzuki de Lima ◽

Natalia Gonçalves Leite ◽

Angela Maria Urrea-Rojas ◽

Andrei Lincoln Yamachita ◽

...

Keyword(s):

Genetic Parameters ◽

Null Alleles ◽

Microsatellite Primers ◽

Neotropical Fish ◽

Genetic Studies ◽

Prochilodus Lineatus ◽

Colossoma Macropomum ◽

Piaractus Mesopotamicus ◽

Future Studies ◽

Environmental Importance

ABSTRACT: Brycon gouldingi is a species of neotropical fish of socioeconomic and environmental importance in the Tocantins-Araguaia Basin. Genetic studies on this species are still limited, making it difficult to evaluate the population structure and genetic diversity in natural and captive stocks. Here, we aimed to evaluate the transferability of heterologous microsatellite primers in B. gouldingi. A total of 30 primers for eight species were evaluated: Brycon hilarii, Brycon opalinus, Brycon cephalus, Brycon orbignyanus, Prochilodus lineatus, Prochilodus argenteus, Piaractus mesopotamicus, and Colossoma macropomum. The primers that showed the best amplification patterns were applied to 20 specimens of B. gouldingi, and their genetic parameters were assessed. Among the 30 primers, seven showed satisfactory transferability, six of which belonged to the genus Brycon: Bh13 (B. hilarii), BoM5, BoM13 (B. opalinus), Borg9, Borg13, and Borg59 (B. orbignyanus), and one belonged to P. argenteus (Par80). The primers for the other species tested showed non-specificity or monomorphism; and were therefore, excluded from the analyses. The number of alleles ranged between two (Borg13 and Borg59) and three (Bh13, BoM5, BoM13, Borg9 and Par80), with sizes varying between 103 bp (BoM5) and 430 bp (Borg9). Four primers showed evidence of null alleles (BoM13, Borg9, Borg13, and Par80), which could probably be attributed to the respective Hardy-Weinberg deviation. Thus, seven primers were validated for cross-amplification in B. gouldingi, which may be used in future studies involving this species.

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Quantitative genetic variation for resistance to the parasite Ichthyophthirius multifiliis in the Neotropical fish tambaqui (Colossoma macropomum)

Aquaculture Reports ◽

10.1016/j.aqrep.2020.100338 ◽

2020 ◽

Vol 17 ◽

pp. 100338 ◽

Cited By ~ 1

Author(s):

Lieschen V.G. Lira ◽

Raquel B. Ariede ◽

Milena V. Freitas ◽

Vito A. Mastrochirico-Filho ◽

John F.G. Agudelo ◽

...

Keyword(s):

Genetic Variation ◽

Ichthyophthirius Multifiliis ◽

Neotropical Fish ◽

Colossoma Macropomum ◽

Quantitative Genetic

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Triploidy in tambaqui Colossoma macropomum identified by chromosomes of fish larvae

Journal of Aquaculture & Marine Biology ◽

10.15406/jamb.2020.09.00279 ◽

2020 ◽

Vol 9 (3) ◽

pp. 65-69

Author(s):

Diogo Hashimoto

Keyword(s):

Fish Larvae ◽

Alternative Methods ◽

Mitotic Chromosomes ◽

Neotropical Fish ◽

Early Puberty ◽

Colossoma Macropomum ◽

Heat Shocks ◽

Negative Impacts ◽

Conservation Plans ◽

Rates Of Growth

Triploid fish are excellent models for use in commercial scale because they can show higher rates of growth, carcass yield and meat quality. If sterile, tambaqui (Colosso mamacropomum) triploids can be used to avoid the problems related to early puberty, especially the negative impacts of male sexual maturation on harvest weight. In the present study, we evaluated the triploidy induction efficiency (thermal shocks) in the fish tambaqui Colossoma macropomum, the most important Neotropical species used for aquaculture. To verify the result of the triploidy experiments, we used a protocol of ploidy identification through analysis of mitotic chromosomes of early life stages (larvae cytogenetics). We obtained better results of hatching and triploid rates through temperature shock at 41°C for 2 min (heat shock) than cold shock. In conclusion, additional treatments will allow to refine the protocols to obtain 100% of triploidy in tambaqui, particularly those from heat shocks. These methodologies can also be applied to other Neotropical fish species, as alternative methods for breeding programs and to delineate conservation plans for the proper management of the stocks.

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