Aquaporin homologs and water transport in the anal papillae of the larval mosquito, Aedes aegypti

2012 ◽  
Vol 182 (8) ◽  
pp. 1047-1056 ◽  
Author(s):  
Jesmilavathani Marusalin ◽  
Brieanne J. Matier ◽  
Mark R. Rheault ◽  
Andrew Donini
Keyword(s):  
Aedes Aegypti ◽  
Water Transport ◽  
Anal Papillae

The Exchange of Labelled Sodium in the Larva of Aedes Aegypti L

1954 ◽  
Vol 31 (3) ◽  
pp. 386-401 ◽  
Author(s):  
JOHN E. TREHERNE
Keyword(s):  
Aedes Aegypti ◽  
Body Surface ◽  
External Medium ◽  
Whole Body ◽  
Effect Of Temperature ◽  
Potassium Ions ◽  
Distilled Water ◽  
General Body ◽  
Anal Papillae

1. The exchange of labelled sodium between the external medium and the haemolymph and whole body has been investigated in the larva of Aedes aegypti. The time for half exchange was of the order of 62 hr. 2. It was found that most of the exchange of labelled sodium occurred through the anal papillae, although smaller amounts enter the haemolymph through the gut and general body surface. Transfer constants have been used to describe the resultant turnover of labelled sodium in the whole system. 3. The rate of uptake of sodium was independent of the external concentrations used in these experiments. 4. Potassium ions do not compete with sodium for uptake, which suggests that separate mechanisms are responsible for the accumulation of these two ions. 5. Larvae were able to retain the sodium in the haemolymph, with relatively little loss, in glass-distilled water. 6. The effect of temperature on the rate of uptake of labelled sodium has been investigated.

scholarly journals The Regulation of Osmotic Pressure and Chloride Concentration in the Haemolymph of Mosquito Larvae

1938 ◽  
Vol 15 (2) ◽  
pp. 235-247 ◽  
Author(s):  
V. B. WIGGLESWORTH
Keyword(s):  
Aedes Aegypti ◽  
Osmotic Pressure ◽  
Culex Pipiens ◽  
Chloride Concentration ◽  
Muscular Activity ◽  
Chloride Content ◽  
Mosquito Larvae ◽  
The Difference ◽  
High Concentrations ◽  
Anal Papillae

The osmotic pressure of the haemolymph in well-fed larvae of Aedes aegypti and Culex pipiens is equivalent to 0.80-0.89% NaCl. During starvation it falls to 0.70% NaCl. The average chloride content of the haemolymph is equivalent to 0.30% NaCl in Aedes, 0.28% in Culex. The non-chloride fraction can be regulated so that the total osmotic pressure remains relatively constant in spite of wide variations in chloride content. The ability of mosquito larvae to take up chloride from dilute solutions (Koch, 1938) is confirmed. In a balanced salt medium both the chloride and osmotic pressure of the blood remain constant until the concentration of the medium reaches o.65-0.75% NaCl. Above this level a variable excess of chloride enters the larva and the total osmotic pressure rises so that it is always a little greater than that of the external medium. At high concentrations (above about 1.6%) the blood chloride rises excessively and all the larvae die. Comparative experiments show that Aedes aegypti is more efficient than Culex pipiens in absorbing and retaining chloride in dilute media, and Culex is perhaps a little better at keeping chloride out in more concentrated media. This difference accords with the difference in their natural breeding places. The anal papillae become greatly enlarged in media almost free from chloride (functional hypertrophy for chloride uptake (Koch, 1938)) and reduced in more concentrated media. The papillae of Culex are more labile in this respect, but the maximum development occurs in Aedes. The ability of larvae to absorb and retain chloride in dilute media is much reduced if they have been reared in more concentrated media. But after transfer to dilute media they soon recover this ability in spite of the reduction in size of the anal papillae. When the larva is deprived of oxygen, muscular activity causes a rise in the osmotic pressure of the blood from about 0.83 to 1.0 or 1.1% NaCl, and this change is associated with extraction of fluid from the tracheoles. But reasons are given for doubting that the level of fluid in the tracheoles is determined by a direct relation between capillarity and osmotic pressure of the blood.

scholarly journals Development of Aedes aegypti (Diptera: Culicidae) mosquito larvae in high ammonia sewage in septic tanks causes alterations in ammonia excretion, ammonia transporter expression, and osmoregulation

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Andrea C. Durant ◽  
Andrew Donini
Keyword(s):  
Aedes Aegypti ◽  
Ammonia Excretion ◽  
Virgin Islands ◽  
Malpighian Tubules ◽  
Comparative Approach ◽  
Vector Mosquito ◽  
High Ammonia ◽  
Rh Proteins ◽  
Anal Papillae ◽  
Excretory Organs

AbstractLarvae of the disease vector mosquito, Aedes aegypti (L.) readily develop in ammonia rich sewage in the British Virgin Islands. To understand how the larvae survive in ammonia levels that are lethal to most animals, an examination of ammonia excretory physiology in larvae collected from septic-water and freshwater was carried out. A. aegypti larvae were found to be remarkably plastic in dealing with high external ammonia through the modulation of NH4+ excretion at the anal papillae, measured using the scanning ion-selective electrode technique (SIET), and NH4+ secretion in the primary urine by the Malpighian tubules when developing in septicwater. Ammonia transporters, Amt and Rh proteins, are expressed in ionoregulatory and excretory organs, with increases in Rh protein, Na+-K+-ATPase, and V-type-H+-ATPase expression observed in the Malpighian tubules, hindgut, and anal papillae in septic-water larvae. A comparative approach using laboratory A. aegypti larvae reared in high ammonia septic-water revealed similar responses to collected A. aegypti with regard to altered ammonia secretion and hemolymph ion composition. Results suggest that the observed alterations in excretory physiology of larvae developing in septic-water is a consequence of the high ammonia levels and that A. aegypti larvae may rely on ammonia transporting proteins coupled to active transport to survive in septic-water.

Studies on the Exchange and Regulation of Sodium in the Larva of Aedes Aegypti (L.)

1960 ◽  
Vol 37 (3) ◽  
pp. 594-608
Author(s):  
R. H. STOBBART
Keyword(s):  
Steady State ◽  
Aedes Aegypti ◽  
Flame Photometry ◽  
Cytological Changes ◽  
The Difference ◽  
Almost All ◽  
Anal Papillae ◽  
Carrier Mechanisms

1. The net transport of sodium into the haemolymph by sodium-deficient 4th-instar larvae of Aëdes aegypti (L.) has been studied by means of flame photometry. The fluxes associated with this net transport have been studied by means of 22Na. 2. The net transport is much more rapid in fed larvae (rate about 50 mM./l./hr.) than in starved larvae (rate about 10 mM./l./hr.). The fluxes are also much greater in the fed larvae. 3. The fluxes associated with net transport in fed and starved larvae are much greater (initially at any rate) than the fluxes occurring in normal fed and starved larvae during steady-state exchange. 4. In both fed and starved larvae almost all the net transport and the fluxes associated with it occur through the anal papillae, so these organs must be responsible for almost all the difference between fed and starved larvae in the rate of net transport and fluxes. 5. Cytological changes in the anal papillae following upon feeding and starvation are described. 6. The results are discussed in terms of possible carrier mechanisms in the anal papillae.

Modes of action of squamocin in the anal papillae of Aedes aegypti larvae

2018 ◽  
Vol 101 ◽  
pp. 172-177 ◽  
Author(s):  
Marilza da Silva Costa ◽  
Sergio Oliveira de Paula ◽  
Gustavo Ferreira Martins ◽  
José Cola Zanuncio ◽  
Antônio Euzébio Goulart Santana ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Modes Of Action ◽  
Anal Papillae

Studies on the Exchange and Regulation of Sodium in the Larva of Aedes Aegypti (L.)

1959 ◽  
Vol 36 (4) ◽  
pp. 641-653
Author(s):  
R. H. STOBBART
Keyword(s):  
Steady State ◽  
Aedes Aegypti ◽  
Instar Larva ◽  
Flame Photometry ◽  
Cytological Changes ◽  
The Difference ◽  
Anal Papillae ◽  
Carrier Mechanisms

1. The steady-state exchange of sodium in the 4th instar larva of Aëdes aegypti (L.) has been studied by means of flame photometry and 22Na. 2. The steady-state exchange of sodium between the larva and medium is more rapid in fed larvae (T½ about 10 hr.) than in starved larvae (T½ about 60 hr.). There is no difference between the sodium levels of fed and starved larvae. 3. In both fed and starved larvae about 90% of the exchange occurs through the anal papillae so these organs must be responsible for about 90% of the difference between fed and starved larvae in the rate of exchange. 4. Cytological changes in the anal papillae following upon feeding and starvation are described. 5. The results are discussed in terms of possible carrier mechanisms in the anal papillae.

ACCUMULATION OF 137Cs BY AEDES AEGYPTI LARVAE AND THE EFFECT OF POTASSIUM CONCENTRATION

1972 ◽  
Vol 104 (3) ◽  
pp. 411-416 ◽  
Author(s):  
J. E. Guthrie ◽  
A. Z. Burzynski
Keyword(s):  
Aedes Aegypti ◽  
Potassium Concentration ◽  
Elimination Rate ◽  
Larval Instars ◽  
Activity Density ◽  
Anal Papillae

AbstractRadiocesium accumulation by Aedes aegypti larvae was greater at 25 °C, than at 20 °C. The activity density (c.p.m./mg dry wt.) of the adults was the same for both sexes, as was the nuclide’s elimination rate. The amount of 137Cs accumulated by the larval instars was inversely proportional to the concentration of potassium in the rearing medium, the suppression of 137Cs uptake being greater at 25 °C than at 20 °C. The possibility that larvae may accumulate 137Cs via the anal papillae and the gut, is briefly discussed.

Sign in / Sign up

Export Citation Format

Share Document