Fructose and stress induce opposite effects on lipid metabolism in the visceral adipose tissue of adult female rats through glucocorticoid action

Abstract Obesity is defined as increased adiposity, which leads to metabolic disease. The growth of adipose tissue depends on its capacity to expand, through hyperplasia or hypertrophy, in order to buffer energy surplus. Also, during the establishment of obesity, adipose tissue expansion reflects adipose lipid metabolism (lipogenesis and/or lipolysis). It is well known that dietary factors can modify lipid metabolism promoting or preventing the development of metabolic abnormalities that concur with obesity. Trans-palmitoleic acid (TP), a biomarker of dairy consumption, has been associated with reduced adiposity in clinical studies. Thus, we aimed to evaluate the effect of TP over adiposity and lipid metabolism-related genes in a rodent model of diet-induced obesity (DIO). To fulfil this aim, we fed C57BL/6 mice with a Control or a High Fat diet, added with or without TP (3g/kg diet), during 11 weeks. Body weight and food intake were monitored, fat pads were weighted, histology of visceral adipose tissue was analysed, and lipid metabolism-related gene expression was explored by qPCR. Results show that TP consumption prevented weight gain induced by high fat diet, reduced visceral adipose tissue weight, and adipocyte size, while increasing the expression of lipolytic molecules. In conclusion, we show for the first time that TP influences adipose tissue metabolism, specifically lipolysis, resulting in decreased adiposity and reduced adipocyte size in a DIO mice model.

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Fructose consumption enhances glucocorticoid action in rat visceral adipose tissue

The Journal of Nutritional Biochemistry ◽

10.1016/j.jnutbio.2012.09.002 ◽

2013 ◽

Vol 24 (6) ◽

pp. 1166-1172 ◽

Cited By ~ 28

Author(s):

Biljana N. Bursać ◽

Ana D. Djordjevic ◽

Ana D. Vasiljević ◽

Danijela D. Vojnović Milutinović ◽

Nataša A. Veličković ◽

...

Keyword(s):

Adipose Tissue ◽

Visceral Adipose Tissue ◽

Visceral Adipose ◽

Glucocorticoid Action

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The human type 2 diabetes-specific visceral adipose tissue proteome and transcriptome in obesity

Scientific Reports ◽

10.1038/s41598-021-96995-0 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Nicholas J. Carruthers ◽

Clarissa Strieder-Barboza ◽

Joseph A. Caruso ◽

Carmen G. Flesher ◽

Nicki A. Baker ◽

...

Keyword(s):

Type 2 Diabetes ◽

Adipose Tissue ◽

Fatty Acid ◽

Lipid Metabolism ◽

Visceral Adipose Tissue ◽

Sequencing Analysis ◽

Complement Factor ◽

Endothelial Protein C Receptor ◽

Visceral Adipose

AbstractDysfunctional visceral adipose tissue (VAT) in obesity is associated with type 2 diabetes (DM) but underlying mechanisms remain unclear. Our objective in this discovery analysis was to identify genes and proteins regulated by DM to elucidate aberrant cellular metabolic and signaling mediators. We performed label-free proteomics and RNA-sequencing analysis of VAT from female bariatric surgery subjects with DM and without DM (NDM). We quantified 1965 protein groups, 23 proteins, and 372 genes that were differently abundant in DM vs. NDM VAT. Proteins downregulated in DM were related to fatty acid synthesis and mitochondrial function (fatty acid synthase, FASN; dihydrolipoyl dehydrogenase, mitochondrial, E3 component, DLD; succinate dehydrogenase-α, SDHA) while proteins upregulated in DM were associated with innate immunity and transcriptional regulation (vitronectin, VTN; endothelial protein C receptor, EPCR; signal transducer and activator of transcription 5B, STAT5B). Transcriptome indicated defects in innate inflammation, lipid metabolism, and extracellular matrix (ECM) function, and components of complement classical and alternative cascades. The VAT proteome and transcriptome shared 13 biological processes impacted by DM, related to complement activation, cell proliferation and migration, ECM organization, lipid metabolism, and gluconeogenesis. Our data revealed a marked effect of DM in downregulating FASN. We also demonstrate enrichment of complement factor B (CFB), coagulation factor XIII A chain (F13A1), thrombospondin 1 (THBS1), and integrins at mRNA and protein levels, albeit with lower q-values and lack of Western blot or PCR confirmation. Our findings suggest putative mechanisms of VAT dysfunction in DM.

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Differential induction of enzymes and genes involved in lipid metabolism in liver and visceral adipose tissue of juvenile yellow catfish Pelteobagrus fulvidraco exposed to copper

Aquatic Toxicology ◽

10.1016/j.aquatox.2013.04.003 ◽

2013 ◽

Vol 136-137 ◽

pp. 72-78 ◽

Cited By ~ 9

Author(s):

Qi-Liang Chen ◽

Zhi Luo ◽

Ya-Xiong Pan ◽

Jia-Lang Zheng ◽

Qing-Ling Zhu ◽

...

Keyword(s):

Adipose Tissue ◽

Lipid Metabolism ◽

Visceral Adipose Tissue ◽

Pelteobagrus Fulvidraco ◽

Yellow Catfish ◽

Induction Of Enzymes ◽

Differential Induction ◽

Visceral Adipose

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Expression of LONP1 Is High in Visceral Adipose Tissue in Obesity, and Is Associated with Glucose and Lipid Metabolism

Endocrinology and Metabolism ◽

10.3803/enm.2021.1023 ◽

2021 ◽

Author(s):

Ju Hee Lee ◽

Saet-Byel Jung ◽

Seong Eun Lee ◽

Ji Eun Kim ◽

Jung Tae Kim ◽

...

Keyword(s):

Adipose Tissue ◽

Lipid Metabolism ◽

Visceral Adipose Tissue ◽

Glucose And Lipid Metabolism ◽

Visceral Adipose

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Adaptation of AMPK-mTOR-signal pathways and lipid metabolism in response to low- and high-rapeseed meal diet in Chinese perch (Siniperca chuatsi)

10.21203/rs.3.rs-17136/v1 ◽

2020 ◽

Author(s):

Jiao Li ◽

Xu-Fang Liang ◽

Muhammad Shoaib Alam ◽

Haocan Luo ◽

Yanpeng Zhang ◽

...

Keyword(s):

Adipose Tissue ◽

Lipid Metabolism ◽

Growth Performance ◽

Mrna Expression ◽

Visceral Adipose Tissue ◽

Rapeseed Meal ◽

Whole Body ◽

Signal Pathways ◽

Chinese Perch ◽

Visceral Adipose

Abstract Background: Chinese perch, a carnivorous fish, can accept artificial diet after domestication nowadays, and this farm way will gain high economic interest and sustainability. However, the high content and high quality requirement of dietary protein make it need the high cost in Chinese perch. Therefore, the aim of this study was to explore the effect of fish meal replacement by low- or high-rapeseed meal on growth performance, feeding, lipid and glucose metabolism. Methods: Three experimental diets were formulated with 0, 10% and 30% rapeseed meal, named as control, RSL and RSH, groups respectively. After the 8-week of feeding trial, growth performance, lipid metabolism and AMPK-mTOR-signal pathways were measured. Results: Chinese perch fed with RSH and RSL diets showed significantly decreased WG, SGR, BFR, VSI, MSI and the whole-body crude lipid compared to those fed with the control diet (P < 0.05). Fish in RSL group decreased feed intake, serum LDL-C, hepatic mRNA expression of LPL, PEPCK and phosphorylated Grb10 (P < 0.05). In visceral adipose tissue, mRNA expression of FAS, SREBP1, ACC1, HL, CPT1 and PEPCK were all significantly down-regulated (P < 0.05). Fish in RSH group showed phosphorylated AMPK, hepatic mRNA expression of SREBP1, ACC1, FAS, PPARα and CPT1 were down-regulated, while HSL, G6PD and PC were up-regulated (P < 0.05). In visceral adipose tissue, mRNA expressions of SREBP1, LPL, CPT1 and PEPCK were down-regulated, while mRNA expression of HSL was up-regulated (P < 0.05). Conclusions: Chinese perch fed with RSL and RSH diets showed decreased fat deposition in viscera. Fish fed with low level of rapeseed meal diet ate less diet, which caused inhibited lipid metabolism in the liver and visceral adipose tissues.Fish fed with high level of rapeseed meal diet inhibited hepatic FA synthesis, activated lipolysis, hence reducing Acetyl-CoA pool. In turn, β-oxidation were inhibited, glycolysis was activated, thus lipid accumulation was decreased. In visceral adipose tissue, lipid uptake was inhibited, caused inhibited FA synthesis, β-oxidation, glycerol synthesis, and improved lipolysis.

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