Increased protein S-nitrosylation in mitochondria: a key mechanism of exercise-induced cardioprotection

The thermoacidophilic archaebacterium Sulfolobus spec. B12 , which is closely related to Sulfolobus solfataricus , possesses a regularly arrayed surface protein (S-layer), which is linked to the plasma membrane via spacer elements spanning a distinct interspace of approximately 18 nm. The S-layer has p3-Symmetry and a lattice constant of 21 nm; three-dimensional reconstructions of negatively stained fragments yield a layer thickness of approximately 6-7 nm.For analysing the molecular architecture of Sulfolobus surface protein in greater detail we use aurothioglucose(ATG)-embedding for specimen preparation. Like glucose, ATG, is supposed to mimic the effect of water, but has the advantage of being less volatile. ATG has advantages over glucose when working with specimens composed exclusively of protein because of its higher density of 2.92 g cm-3. Because of its high radiation sensitivity electromicrographs has to be recorded under strict low-dose conditions. We have recorded electromicrographs with a liquid helium-cooled superconducting electron microscope (the socalled SULEIKA at the Fritz-Haber-lnstitut) with a specimen temperature of 4.5 K and with a maximum dose of 2000 e nm-2 avoiding any pre-irradiation of the specimen.

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A colon epithelial protein(s) induces oral tolerance in a dose dependent manner in the trinitrobenzene sulfonic acid (TNBS) model of colitis in rats

Gastroenterology ◽

10.1016/s0016-5085(01)81385-8 ◽

2001 ◽

Vol 120 (5) ◽

pp. A279-A279

Author(s):

A DASGUPTA ◽

J GIRALDO ◽

P AMENTA ◽

K DAS

Keyword(s):

Sulfonic Acid ◽

Oral Tolerance ◽

Protein S ◽

Trinitrobenzene Sulfonic Acid ◽

Dependent Manner ◽

Dose Dependent

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M.681 Clinical relevance of identification of exercise-induced hypertension in adults who received surgical correction of aortic coarctation in childhood

Atherosclerosis ◽

10.1016/s0021-9150(04)90679-x ◽

2004 ◽

Vol 5 (1) ◽

pp. 157

Author(s):

J VRIEND

Keyword(s):

Clinical Relevance ◽

Aortic Coarctation ◽

Surgical Correction ◽

Induced Hypertension ◽

Exercise Induced

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Effects of Antioxidant Supplementation and Exercise Training on Erythrocyte Antioxidant Enzymes

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831.76.5.324 ◽

2006 ◽

Vol 76 (5) ◽

pp. 324-331 ◽

Cited By ~ 11

Author(s):

Marsh ◽

Laursen ◽

Coombes

Keyword(s):

Antioxidant Enzymes ◽

Exercise Training ◽

Endurance Training ◽

Young Male ◽

Antioxidant Defenses ◽

Antioxidant Enzyme Activities ◽

Antioxidant Supplementation ◽

Male Wistar Rats ◽

Exercise Induced ◽

Antioxidant Supplements

Erythrocytes transport oxygen to tissues and exercise-induced oxidative stress increases erythrocyte damage and turnover. Increased use of antioxidant supplements may alter protective erythrocyte antioxidant mechanisms during training. Aim of study: To examine the effects of antioxidant supplementation (α-lipoic acid and α-tocopherol) and/or endurance training on the antioxidant defenses of erythrocytes. Methods: Young male Wistar rats were assigned to (1) sedentary; (2) sedentary and antioxidant-supplemented; (3) endurance-trained; or (4) endurance-trained and antioxidant-supplemented groups for 14 weeks. Erythrocyte superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) activities, and plasma malondialdehyde (MDA) were then measured. Results: Antioxidant supplementation had no significant effect (p > 0.05) on activities of antioxidant enzymes in sedentary animals. Similarly, endurance training alone also had no effect (p > 0.05). GPX (125.9 ± 2.8 vs. 121.5 ± 3.0 U.gHb–1, p < 0.05) and CAT (6.1 ± 0.2 vs. 5.6 ± 0.2 U.mgHb–1, p < 0.05) activities were increased in supplemented trained animals compared to non-supplemented sedentary animals whereas SOD (61.8 ± 4.3 vs. 52.0 ± 5.2 U.mgHb–1, p < 0.05) activity was decreased. Plasma MDA was not different among groups (p > 0.05). Conclusions: In a rat model, the combination of exercise training and antioxidant supplementation increased antioxidant enzyme activities (GPX, CAT) compared with each individual intervention.

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Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000654 ◽

2020 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

Masoud Nasiri ◽

Saja Ahmadizad ◽

Mehdi Hedayati ◽

Tayebe Zarekar ◽

Mehdi Seydyousefi ◽

...

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Wistar Rats ◽

Enzymatic Antioxidants ◽

Acute Response ◽

Total Antioxidant ◽

Male Wistar Rats ◽

Exercise Induced ◽

And Control

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.

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