Marked microglial reaction in normal aging human substantia nigra: correlation with extraneuronal neuromelanin pigment deposits

2007 ◽  
Vol 114 (4) ◽  
pp. 419-424 ◽  
Author(s):  
Thomas G. Beach ◽  
Lucia I. Sue ◽  
Douglas G. Walker ◽  
Lih Fen Lue ◽  
Donald J. Connor ◽  
...  
Keyword(s):  
Substantia Nigra ◽  
Normal Aging ◽  
Human Substantia Nigra

Cytochrome c oxidase defects of the human substantia nigra in normal aging

1996 ◽  
Vol 17 (6) ◽  
pp. 843-848 ◽  
Author(s):  
Kyoko Itoh ◽  
Serge Weis ◽  
Parviz Mehraein ◽  
Josef Müller-Höcker
Keyword(s):  
Cytochrome C ◽  
Substantia Nigra ◽  
Cytochrome C Oxidase ◽  
Normal Aging ◽  
Human Substantia Nigra

scholarly journals Anatomical and functional organization of the human substantia nigra and its connections

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Yu Zhang ◽  
Kevin Michel-Herve Larcher ◽  
Bratislav Misic ◽  
Alain Dagher
Keyword(s):  
Substantia Nigra ◽  
Functional Organization ◽  
Impulsive Choice ◽  
Behavioral Measures ◽  
Human Connectome Project ◽  
Motor Impulsivity ◽  
Cortical Regions ◽  
Value Coding ◽  
Decisional Impulsivity ◽  
Human Substantia Nigra

We investigated the anatomical and functional organization of the human substantia nigra (SN) using diffusion and functional MRI data from the Human Connectome Project. We identified a tripartite connectivity-based parcellation of SN with a limbic, cognitive, motor arrangement. The medial SN connects with limbic striatal and cortical regions and encodes value (greater response to monetary wins than losses during fMRI), while the ventral SN connects with associative regions of cortex and striatum and encodes salience (equal response to wins and losses). The lateral SN connects with somatomotor regions of striatum and cortex and also encodes salience. Behavioral measures from delay discounting and flanker tasks supported a role for the value-coding medial SN network in decisional impulsivity, while the salience-coding ventral SN network was associated with motor impulsivity. In sum, there is anatomical and functional heterogeneity of human SN, which underpins value versus salience coding, and impulsive choice versus impulsive action.

Nucleolin in Neurons of the Human Substantia Nigra

2018 ◽  
Vol 12 (2) ◽  
pp. 205-208
Author(s):  
D. A. Sufieva ◽  
V. V. Guselnikova ◽  
D. E. Korzhevskii
Keyword(s):  
Substantia Nigra ◽  
Human Substantia Nigra

Distribution of [3H]SCH 23390 Binding Sites in the Human Substantia Nigra

1991 ◽  
pp. 93-94
Author(s):  
F. Thibaut ◽  
E.C. Hirsch ◽  
R. Raisman ◽  
F. Javoy-Agid ◽  
Y. Agid
Keyword(s):  
Substantia Nigra ◽  
Binding Sites ◽  
Sch 23390 ◽  
Human Substantia Nigra

scholarly journals Quantitative Profiling of the Human Substantia Nigra Proteome from Laser-capture Microdissected FFPE Tissue

2020 ◽  
Vol 19 (5) ◽  
pp. 839-851 ◽  
Author(s):  
Eva Griesser ◽  
Hannah Wyatt ◽  
Sara Ten Have ◽  
Birgit Stierstorfer ◽  
Martin Lenter ◽  
...  
Keyword(s):  
Substantia Nigra ◽  
Solid Phase ◽  
Neuronal Cell ◽  
Alpha Synuclein ◽  
Ffpe Tissue ◽  
Specific Gene ◽  
Tissue Specimens ◽  
Laser Capture ◽  
Go Terms ◽  
Human Substantia Nigra

Laser-capture microdissection (LCM) allows the visualization and isolation of morphologically distinct subpopulations of cells from heterogeneous tissue specimens. In combination with formalin-fixed and paraffin-embedded (FFPE) tissue it provides a powerful tool for retrospective and clinically relevant studies of tissue proteins in a healthy and diseased context. We first optimized the protocol for efficient LCM analysis of FFPE tissue specimens. The use of SDS containing extraction buffer in combination with the single-pot solid-phase-enhanced sample preparation (SP3) digest method gave the best results regarding protein yield and protein/peptide identifications. Microdissected FFPE human substantia nigra tissue samples (∼3,000 cells) were then analyzed, using tandem mass tag (TMT) labeling and LC-MS/MS, resulting in the quantification of >5,600 protein groups. Nigral proteins were classified and analyzed by abundance, showing an enrichment of extracellular exosome and neuron-specific gene ontology (GO) terms among the higher abundance proteins. Comparison of microdissected samples with intact tissue sections, using a label-free shotgun approach, revealed an enrichment of neuronal cell type markers, such as tyrosine hydroxylase and alpha-synuclein, as well as proteins annotated with neuron-specific GO terms. Overall, this study provides a detailed protocol for laser-capture proteomics using FFPE tissue and demonstrates the efficiency of LCM analysis of distinct cell subpopulations for proteomic analysis using low sample amounts.

scholarly journals Novelty-Sensitive Dopaminergic Neurons in the Human Substantia Nigra Predict Success of Declarative Memory Formation

2018 ◽  
Vol 28 (9) ◽  
pp. 1333-1343.e4 ◽  
Author(s):  
Jan Kamiński ◽  
Adam N. Mamelak ◽  
Kurtis Birch ◽  
Clayton P. Mosher ◽  
Michele Tagliati ◽  
...  
Keyword(s):  
Substantia Nigra ◽  
Dopaminergic Neurons ◽  
Declarative Memory ◽  
Memory Formation ◽  
Human Substantia Nigra
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