Myelination by transplanted human and mouse central nervous system tissue after long-term cryopreservation

1995 ◽  
Vol 91 (1) ◽  
pp. 82-88 ◽  
Author(s):  
D. Seilhean ◽  
A. Gansmüller ◽  
A. Baron-Van Evercooren ◽  
M. Gumpel ◽  
F. Lachapelle
1997 ◽  
Vol 3 (4) ◽  
pp. 271-285 ◽  
Author(s):  
Francoise Fouquet ◽  
Jia Min Zhou ◽  
Evelyn Ralston ◽  
Kerren Murray ◽  
Frédéric Troalen ◽  
...  

ASN NEURO ◽  
2016 ◽  
Vol 8 (5) ◽  
pp. 175909141667097 ◽  
Author(s):  
Scott A. Sands ◽  
Regis Leung-Toung ◽  
Yingsheng Wang ◽  
John Connelly ◽  
Steven M. LeVine

Author(s):  
J.N. Turner ◽  
M. Siemens ◽  
D. Szarowski ◽  
D.N. Collins

A classic preparation of central nervous system tissue (CNS) is the Golgi procedure popularized by Cajal. The method is partially specific as only a few cells are impregnated with silver chromate usualy after osmium post fixation. Samples are observable by light (LM) or electron microscopy (EM). However, the impregnation is often so dense that structures are masked in EM, and the osmium background may be undesirable in LM. Gold toning is used for a subtle but high contrast EM preparation, and osmium can be omitted for LM. We are investigating these preparations as part of a study to develop correlative LM and EM (particularly HVEM) methodologies in neurobiology. Confocal light microscopy is particularly useful as the impregnated cells have extensive three-dimensional structure in tissue samples from one to several hundred micrometers thick. Boyde has observed similar preparations in the tandem scanning reflected light microscope (TSRLM).


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