Human melanocytes grown in epidermal equivalents transfer their melanin to follicular outer root sheath keratinocytes

1999 ◽  
Vol 291 (6) ◽  
pp. 325-332 ◽  
Author(s):  
A. Limat ◽  
Denis Salomon ◽  
Pierre Carraux ◽  
Jean-Hilaire Saurat ◽  
Thomas Hunziker



2019 ◽  
Vol 30 (5) ◽  
pp. 365 ◽  
Author(s):  
Saki Onishi ◽  
Yuta Baba ◽  
Fumika Yokoi ◽  
Kaori Ide ◽  
Manabu Ohyama ◽  
...  


1991 ◽  
Vol 283 (7) ◽  
pp. 476-479 ◽  
Author(s):  
F. Kiesewetter ◽  
A. Arai ◽  
J. v. Hintzenstern ◽  
H. Schell


2015 ◽  
Vol 23 (4) ◽  
pp. 601-610 ◽  
Author(s):  
Li Yan ◽  
Rui Cao ◽  
Lianzhao Wang ◽  
Yuanbo Liu ◽  
Bo Pan ◽  
...  


2020 ◽  
Vol 140 (5) ◽  
pp. 1085-1088.e6 ◽  
Author(s):  
Erika Lisztes ◽  
Balázs István Tóth ◽  
Marta Bertolini ◽  
Imre Lőrinc Szabó ◽  
Nóra Zákány ◽  
...  


Processes ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 177
Author(s):  
Hanluo Li ◽  
Jule Kristin Michler ◽  
Alexander Bartella ◽  
Anna Katharina Sander ◽  
Sebastian Gaus ◽  
...  

Hair follicles harbor a heterogeneous regenerative cell pool and represent a putative low-to-non-invasively available source of stem cells. We previously reported a technology for culturing human melanocytes from the hair follicle outer root sheath (ORS) for autologous pigmentation of tissue engineered skin equivalents. This study translated the ORS technology to horses. We de-veloped a culture of equine melanocytes from the ORS (eMORS) from equine forelock hair follicles cultured by means of an analogue human hair follicle-based in vitro methodology. The procedure was adjusted to equine physiology by addition of equine serum to the culture medium. The hair follicles were isolated by macerating forelock skin rests, enzymatically digested and subjected to air-medium-interface cultivation method. The procedure resulted in differentiated equine melanocytes, which exhibited typical morphology, presence of melanosomes, expression of cytoskeleton proteins vimentin, α-SMA, Sox2, S100ß and tyrosinase as well as tyrosinase activity followed by production of melanin. According to all assessed parameters, eMORS could be ranked as partially melanotic melanocytes. The results of the study offer an experimental base for further insight into hair follicle biology in equine and for comparative studies of hair follicles across different species.







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