An osteopontin‐derived peptide inhibits human hair growth at least in part by decreasing fibroblast growth factor‐7 production in outer root sheath keratinocytes

Hair growth and morphology are generally regulated by the hair cycle in mammals. Fibroblast Growth Factor 5 (FGF5), which is a hair cycle regulator, has a role in regulating the hair cycle during the transition from the anagen phase to the catagen phase, and a hereditary long hair phenotype has been widely reported when FGF5 is mutated in humans and other species. However, there has been no such report in rabbits. Thus, the first exon of rabbit FGF5 was disrupted by the CRISPR/Cas9 system, and the phenotype of FGF5-/- rabbits was characterized while using hematoxylin and eosin (H&E) staining, immunohistochemistry, quantitative PCR, scanning electron microscopy, and western blotting. The results showed a significant and systemic long hair phenotype in the FGF5-/- rabbits, which indicated that FGF5 is a negative regulator of hair growth. In addition, a decreased diameter of the fiber and a higher area proportion of hair follicle clusters were determined in FGF5-/- rabbits as compared with the WT rabbits. Further investigation verified that prolonging the anagen phase in rabbits, with decreased BMP2/4 pathway signaling and increased VERSICAN pathway signaling, caused the systemic long hair phenotype. Taken together, these results indicate a systemic long hair phenotype by prolonging anagen in FGF5-/- rabbits, which could be widely used for Fur production and an ideal model for studying the mechanism of long hair in the future.

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Decapeptide with fibroblast growth factor (FGF)-5 partial sequence inhibits hair growth suppressing activity of FGF-5

Journal of Cellular Physiology ◽

10.1002/jcp.10369 ◽

2003 ◽

Vol 197 (2) ◽

pp. 272-283 ◽

Cited By ~ 18

Author(s):

Chikako Ito ◽

Yuko Saitoh ◽

Yasuko Fujita ◽

Yoshimitsu Yamazaki ◽

Toru Imamura ◽

...

Keyword(s):

Growth Factor ◽

Fibroblast Growth Factor ◽

Hair Growth ◽

Partial Sequence ◽

Fibroblast Growth

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Effects of testosterone, dihydrotestosterone and estradiol on growth of human hair outer root sheath keratinocytes in vitro

Archives of Dermatological Research ◽

10.1007/bf00371787 ◽

1991 ◽

Vol 283 (7) ◽

pp. 476-479 ◽

Cited By ~ 8

Author(s):

F. Kiesewetter ◽

A. Arai ◽

J. v. Hintzenstern ◽

H. Schell

Keyword(s):

Human Hair ◽

Outer Root Sheath ◽

Root Sheath ◽

Outer Root Sheath Keratinocytes

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Fibroblast Growth Factors Stimulate Hair Growth throughβ-Catenin and Shh Expression in C57BL/6 Mice

BioMed Research International ◽

10.1155/2015/730139 ◽

2015 ◽

Vol 2015 ◽

pp. 1-9 ◽

Cited By ~ 10

Author(s):

Wei-hong Lin ◽

Li-Jun Xiang ◽

Hong-Xue Shi ◽

Jian Zhang ◽

Li-ping Jiang ◽

...

Keyword(s):

Growth Factors ◽

Growth Factor ◽

Fibroblast Growth Factor ◽

Hair Growth ◽

Hair Follicles ◽

Control Group ◽

Fibroblast Growth ◽

Acidic Fibroblast Growth Factor ◽

Anagen Phase ◽

Growth Promoting

Growth factors are involved in the regulation of hair morphogenesis and cycle hair growth. The present study sought to investigate the hair growth promoting activities of three approved growth factor drugs, fibroblast growth factor 10 (FGF-10), acidic fibroblast growth factor (FGF-1), and basic fibroblast growth factor (FGF-2), and the mechanism of action. We observed that FGFs promoted hair growth by inducing the anagen phase in telogenic C57BL/6 mice. Specifically, the histomorphometric analysis data indicates that topical application of FGFs induced an earlier anagen phase and prolonged the mature anagen phase, in contrast to the control group. Moreover, the immunohistochemical analysis reveals earlier induction ofβ-catenin and Sonic hedgehog (Shh) in hair follicles of the FGFs-treated group. These results suggest that FGFs promote hair growth by inducing the anagen phase in resting hair follicles and might be a potential hair growth-promoting agent.

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