Adenosine Promotes Human Hair Growth and Inhibits Catagen Transition In Vitro: Role of the Outer Root Sheath Keratinocytes

Rab27a/b are known to play an important role in the transport of melanosomes, with their knockout causing silvery gray hair. However, the relationship between Rab27a/b and hair growth is not well known. To evaluate the role of Rab27a/b in hair cycle, we investigated the expression of Rab27a/b during hair cycling and human outer root sheath (hORS) cells. The expression of Rab27a in ORS cells was mainly detected at the anagen, whereas expression of Rab27b in ORS, and epidermal cells was strongly expressed at the telogen. Additionally, Rab27a/b were expressed in the Golgi of hORS cells. To evaluate the role of Rab27a/b in hair growth, telogen-to-anagen transition animal and vibrissae hair follicles (HFs) organ culture models were assayed using Rab27a/b siRNAs. The knockdown of Rab27a or Rab27b suppressed or promoted hair growth, respectively. These results were also confirmed in human dermal papilla cells (hDPCs) and hORS cells, showing the opposite mitogenic effects. Moreover, Rab27b knockdown increased the expression levels of various growth factors in the hDPCs and hORS cells. Overall, the opposite temporal expression patterns during hair cycling and roles for hair growth of Rab27a/b suggested that Rab27a/b might regulate the hair cycle. Therefore, our study may provide a novel solution for the development of hair loss treatment by regulating Rab27a/b levels.

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Enhanced Proliferation and Functions of In Vitro Expanded Human Hair Follicle Outer Root Sheath Cells by Low Oxygen Tension Culture

Tissue Engineering Part C Methods ◽

10.1089/ten.tec.2011.0489 ◽

2012 ◽

Vol 18 (8) ◽

pp. 603-613 ◽

Cited By ~ 1

Author(s):

Lingling Xia ◽

Qing Liu ◽

Wenjie Zhang ◽

Guangdong Zhou ◽

Yilin Cao ◽

...

Keyword(s):

Hair Follicle ◽

Oxygen Tension ◽

Human Hair ◽

Low Oxygen ◽

Human Hair Follicle ◽

Outer Root Sheath ◽

Root Sheath ◽

Low Oxygen Tension ◽

Outer Root Sheath Cells

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Expression and role of E- and P-cadherin adhesion molecules in embryonic histogenesis. II. Skin morphogenesis

Development ◽

10.1242/dev.105.2.271 ◽

1989 ◽

Vol 105 (2) ◽

pp. 271-277 ◽

Cited By ~ 2

Author(s):

Y. Hirai ◽

A. Nose ◽

S. Kobayashi ◽

M. Takeichi

Keyword(s):

Hair Follicles ◽

Homogeneous Distribution ◽

Upper Lip ◽

Outer Root Sheath ◽

Root Sheath ◽

Skin Morphogenesis ◽

Dermal Cells ◽

E Cadherin

Expression and the role of E- and P-cadherin in the histogenesis of the surface epidermis and hair follicles were examined using the upper lip skin of the mouse. P-cadherin is expressed exclusively in the proliferating region of these tissues, that is in the germinative layer of the surface epidermis, the outer root sheath and the hair matrix. E-cadherin is coexpressed in these layers but this molecule was also detected in non-proliferating regions such as the intermediate layer of the surface epidermis and the immature regions of the inner root sheath. Neither P- nor E-cadherin was detected in fully keratinized layers such as the horny layer of the surface epidermis, the outermost layer of the outer root sheath and the mature hair fibres. These two cadherins were not detected in dermal cells. We cultured pieces of the upper lip skin in vitro in the absence or presence of a monoclonal antibody to E-cadherin (ECCD-1) or to P-cadherin (PCD-1). In control cultures, skin morphogenesis normally occurred in a pattern whereby the hair follicles grew and dermal cells were condensed to form the dermal sheath. A mixture of ECCD-1 and PCD-1, however, induced abnormal morphogenesis in the skin in several respects. (1) The cuboidal or columnar arrangement of basal epithelial cells was distorted. (2) Hair follicles were deformed. (3) Condensation of dermal cells was suppressed, causing a homogeneous distribution of these cells. These results suggest that cadherins present in epidermal cells are involved not only in maintaining the arrangement of these cells but also in inducing dermal condensation.

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