HMGB1 gene knockout in mouse embryonic fibroblasts results in reduced telomerase activity and telomere dysfunction

Chromosoma ◽  
2012 ◽  
Vol 121 (4) ◽  
pp. 419-431 ◽  
Author(s):  
Eva Polanská ◽  
Zuzana Dobšáková ◽  
Martina Dvořáčková ◽  
Jiří Fajkus ◽  
Michal Štros
Keyword(s):  
Gene Knockout ◽  
Telomerase Activity ◽  
Telomere Dysfunction ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts

Effect of PRAK gene knockout on the proliferation of mouse embryonic fibroblasts

2009 ◽  
Vol 3 (4) ◽  
pp. 379-383 ◽  
Author(s):  
Xiaowei Gong ◽  
Aihua Liu ◽  
Xiaoyan Ming ◽  
Xu Wang ◽  
Daan Wang ◽  
...  
Keyword(s):  
Gene Knockout ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts

scholarly journals Functional specialization of calreticulin domains

2001 ◽  
Vol 154 (5) ◽  
pp. 961-972 ◽  
Author(s):  
Kimitoshi Nakamura ◽  
Anna Zuppini ◽  
Serge Arnaudeau ◽  
Jeffery Lynch ◽  
Irfan Ahsan ◽  
...  
Keyword(s):  
Storage Capacity ◽  
Gene Knockout ◽  
Cell Surface Receptor ◽  
Functional Specialization ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Inositol 1,4,5 Trisphosphate ◽  
Measurable Level ◽  
Surface Receptor ◽  
Lower Capacity

Calreticulin is a Ca2+-binding chaperone in the endoplasmic reticulum (ER), and calreticulin gene knockout is embryonic lethal. Here, we used calreticulin-deficient mouse embryonic fibroblasts to examine the function of calreticulin as a regulator of Ca2+ homeostasis. In cells without calreticulin, the ER has a lower capacity for Ca2+ storage, although the free ER luminal Ca2+ concentration is unchanged. Calreticulin-deficient cells show inhibited Ca2+ release in response to bradykinin, yet they release Ca2+ upon direct activation with the inositol 1,4,5-trisphosphate (InsP3). These cells fail to produce a measurable level of InsP3 upon stimulation with bradykinin, likely because the binding of bradykinin to its cell surface receptor is impaired. Bradykinin binding and bradykinin-induced Ca2+ release are both restored by expression of full-length calreticulin and the N + P domain of the protein. Expression of the P + C domain of calreticulin does not affect bradykinin-induced Ca2+ release but restores the ER Ca2+ storage capacity. Our results indicate that calreticulin may play a role in folding of the bradykinin receptor, which affects its ability to initiate InsP3-dependent Ca2+ release in calreticulin-deficient cells. We concluded that the C domain of calreticulin plays a role in Ca2+ storage and that the N domain may participate in its chaperone functions.

CRISPR/Cas9 mediated gene knockout of Glb1 gene in mouse embryonic fibroblasts

Gene Reports ◽  
2019 ◽  
Vol 16 ◽  
pp. 100419
Author(s):  
Ali Zarei ◽  
Vahid Razban ◽  
Seyed Mohammad Bagher Tabei ◽  
Seyed Ebrahim Hosseini
Keyword(s):  
Gene Knockout ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Glb1 Gene

scholarly journals Relative importance of βcyto- and γcyto-actin in primary mouse embryonic fibroblasts

2017 ◽  
Vol 28 (6) ◽  
pp. 771-782 ◽  
Author(s):  
Xiaobai Patrinostro ◽  
Allison R. O'Rourke ◽  
Christopher M. Chamberlain ◽  
Branden S. Moriarity ◽  
Benjamin J. Perrin ◽  
...  
Keyword(s):  
Gene Targeting ◽  
Gene Knockout ◽  
T Antigen ◽  
Mouse Embryonic Fibroblasts ◽  
Double Knockout ◽  
Embryonic Fibroblasts ◽  
Primary Fibroblast ◽  
Primary Mouse ◽  
Primary Fibroblasts ◽  
Actin Isoform

The highly homologous β (βcyto) and γ (γcyto) cytoplasmic actins are hypothesized to carry out both redundant and unique essential functions, but studies using targeted gene knockout and siRNA-mediated transcript knockdown to examine βcyto- and γcyto-isoform–­specific functions in various cell types have yielded conflicting data. Here we quantitatively characterized actin transcript and protein levels, as well as cellular phenotypes, in both gene- and transcript-targeted primary mouse embryonic fibroblasts. We found that the smooth muscle αsm-actin isoform was the dominantly expressed actin isoform in WT primary fibroblasts and was also the most dramatically up-regulated in primary βcyto- or β/γcyto-actin double-knockout fibroblasts. Gene targeting of βcyto-actin, but not γcyto-actin, led to greatly decreased cell proliferation, decreased levels of cellular ATP, and increased serum response factor signaling in primary fibroblasts, whereas immortalization induced by SV40 large T antigen supported fibroblast proliferation in the absence of βcyto-actin. Consistent with in vivo gene-targeting studies in mice, both gene- and transcript-targeting approaches demonstrate that the loss of βcyto-actin protein is more disruptive to primary fibroblast function than is the loss of γcyto-actin.

scholarly journals Regulation of c-Fos Gene Expression by NF-κB: A p65 Homodimer Binding Site in Mouse Embryonic Fibroblasts but Not Human HEK293 Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (12) ◽  
pp. e84062 ◽  
Author(s):  
Yu-Cheng Tu ◽  
Duen-Yi Huang ◽  
Shine-Gwo Shiah ◽  
Jang-Shiun Wang ◽  
Wan-Wan Lin
Keyword(s):  
Gene Expression ◽  
Binding Site ◽  
Hek293 Cells ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts

scholarly journals Introduction of Mouse Embryonic Fibroblasts into Early Embryos: From Confusion to Constructive Discussion. Comment on Savatier, P. Introduction of Mouse Embryonic Fibroblasts into Early Embryos Causes Reprogramming and (Con)fusion. Cells 2021, 10, 772

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1534
Author(s):  
Krystyna Żyżyńska-Galeńska ◽  
Jolanta Karasiewicz ◽  
Agnieszka Bernat
Keyword(s):  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Early Embryos ◽  
Constructive Discussion

We would like to address the issues raised by Pierre Savatier in “Introduction of Mouse Embryonic Fibroblasts into Early Embryos Causes Reprogramming and (Con)Fusion” [...]

616 Mouse Embryonic Fibroblasts Null for the KrüPpel-Like Factor 4 Alleles Are Genetically Unstable

2008 ◽  
Vol 134 (4) ◽  
pp. A-86
Author(s):  
Engda G. Hagos ◽  
Amr Ghaleb ◽  
W Brian Dalton ◽  
Jonathan P. Katz ◽  
Klaus H. Kaestner ◽  
...  
Keyword(s):  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts

scholarly journals Introduction of Mouse Embryonic Fibroblasts into Early Embryos Causes Reprogramming and (Con)fusion

Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 772
Author(s):  
Pierre Savatier
Keyword(s):  
Somatic Cell ◽  
Cell Nuclei ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Early Embryos

The reprogramming of somatic cell nuclei to achieve pluripotency is one of the most important biological discoveries of the last few decades [...]

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