scholarly journals Complex challenges of estimating the age and vitality of muscle wounds: a study with matrix metalloproteinases and their inhibitors on animal and human tissue samples

2021 ◽  
Author(s):  
A. Niedecker ◽  
R. Huhn ◽  
St. Ritz-Timme ◽  
F. Mayer
Keyword(s):  
Matrix Metalloproteinases ◽  
Human Tissue ◽  
Myocardial Infarctions ◽  
Wound Age ◽  
Tissue Samples ◽  
Forensic Research ◽  
Routine Work ◽  
Long Time ◽  
Reliable Marker ◽  
Sample Set

AbstractThe estimation of wound age and wound vitality is a recurring task in forensic routine work and has been subject of forensic research for a long time. By now, an unrestrictedly reliable marker or set of markers has not been found. In a study on myocardial infarctions, matrix metalloproteinases (MMP) 2 and 9 as well as tissue inhibitor of matrix metalloproteinases 1 (TIMP-1) were detected immunohistochemically in mechanically wounded myocardium (ECG electrodes, vessel ligations). Against this background, the potency of MMP-9, MMP-2, and TIMP-1 as markers for the estimation of wound age and wound vitality was tested in a broad approach with human tissue samples drawn during autopsies and with an animal model, the isolated perfused Langendorff heart. The study comprised samples of injured human skeletal muscle, injured human myocardium, rats’ hearts with vital wounds, and rats’ hearts with postmortem-inflicted wounds that were all stained immunohistochemically. The results showed great scattering, leading to the conclusion that MMP-2, MMP-9, and TIMP-1 are not suitable for wound age estimation. Merely the results for TIMP-1 suggested that this marker might be able to differentiate between vital and postmortem-inflicted wounds. With a view to the promising results of the preceding study, the results underline the necessity to test possible markers of wound age/wound vitality on a large and diverse sample set.

Scintigraphic Detection of Experimental Myocardial Infarction with 99mTc-2,3-Dimercaptosuccinic Acid

1984 ◽  
Vol 23 (06) ◽  
pp. 317-319
Author(s):  
J. Novák ◽  
Y. Mazurová ◽  
J. Kubíček ◽  
J. Yižd’a ◽  
P. Kafka ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Coronary Artery ◽  
Intravenous Administration ◽  
Experimental Myocardial Infarction ◽  
Myocardial Infarctions ◽  
Clinical Use ◽  
Scintigraphic Imaging ◽  
Tissue Samples ◽  
Scintigraphic Finding

SummaryAcute myocardial infarctions were produced by ligature of the left frontal descending coronary artery in 9 dogs. The possibility of scintigraphic imaging with 99mTc-DMSA 4 hrs after intravenous administration was studied. The infarctions were 4, 24 and 48 hrs old. The in vivo scan was positive in only one dog with a 4-hr old infarction. The in vivo scans were confirmed by the analysis of the radioactivity in tissue samples. The accumulation of the radiopharmaceutical increased slightly in 48-hr old lesions; however, this increase was not sufficient for a positive scintigraphic finding. Thus, we do not recommend 99mTc-DMSA for clinical use in acute lesions.

Analysis of ∼700 Human Tissue Samples Identifies microRNAs That Are Associated with, Predictive of, and Necessary for Insulin Gene Transcription

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 2160-P
Author(s):  
ANAND HARDIKAR ◽  
WILSON WONG ◽  
MUGDHA JOGLEKAR ◽  
LOUISE T. DALGAARD ◽  
ALICIA JENKINS ◽  
...  
Keyword(s):  
Gene Transcription ◽  
Human Tissue ◽  
Insulin Gene ◽  
Tissue Samples ◽  
Insulin Gene Transcription

scholarly journals Human tissue samples more difficult to obtain for academics

2002 ◽  
Vol 8 (6) ◽  
pp. 543-543
Author(s):  
David Dickson
Keyword(s):  
Human Tissue ◽  
Tissue Samples

Matrix metalloproteinases-2 and -9 in cervical cancer: different roles in tumor progression

2006 ◽  
Vol 16 (3) ◽  
pp. 1297-1302 ◽  
Author(s):  
M. Rauvala ◽  
K. Aglund ◽  
U. Puistola ◽  
T. Turpeenniemi-Hujanen ◽  
G. Horvath ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Matrix Metalloproteinases ◽  
Young Women ◽  
Uterine Cervical Cancer ◽  
University Hospital ◽  
High Grade ◽  
Tissue Samples ◽  
Primary Tumors ◽  
Early Sexual Activity ◽  
Kaplan Meier

The incidence of uterine cervical cancer has increased slightly in Western countries, with an increase in relatively young women. Overexpression of matrix metalloproteinases (MMPs)-2 and -9 has turned out as a prognostic factor in many cancers. We compared the expression of the proteins MMP-2 and MMP-9 in cervical primary tumors with clinical outcome and risk factors of cervical cancer. One hundred sixty-one patients with cervical cancer treated in Umeå University Hospital or Sahlgrenska University Hospital, Sweden, between 1991 and 1995 were included in the study. Paraffin-embedded tissue samples obtained prior to treatment were examined immunohistochemically by specific antibodies for MMP-2 and MMP-9. Forty-two percent of the tumors were intensively positive for MMP-2 and 31% for MMP-9. Nineteen percent of the samples were intensively positive for both proteinases and 47% negative or weak for both. Overexpression of MMP-2 seemed to predict unfavorable survival under Kaplan–Meier analysis and in the multivariate analysis. Early sexual activity and low parity seemed to correlate to overexpression of MMP-2. MMP-9 was not associated with survival or sexual behavior. Intensive MMP-9 was noted in grade 1 tumors. We conclude that MMP-2 and MMP-9 have different roles in uterine cervical cancer. MMP-2 could be associated with aggressive behavior, but MMP-9 expression diminishes in high-grade tumors.

Ultra-trace analysis of platinum in human tissue samples

2005 ◽  
Vol 382 (7) ◽  
pp. 1500-1506 ◽  
Author(s):  
Elisabeth Rudolph ◽  
Stephan Hann ◽  
Gerhard Stingeder ◽  
Christian Reiter
Keyword(s):  
Trace Analysis ◽  
Human Tissue ◽  
Tissue Samples ◽  
Ultra Trace ◽  
Ultra Trace Analysis

scholarly journals Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot

Biology ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 59 ◽  
Author(s):  
Magali Humbert ◽  
María Morán ◽  
Patricia de la Cruz-Ojeda ◽  
Jordi Muntané ◽  
Tabea Wiedmer ◽  
...  
Keyword(s):  
Human Tissue ◽  
Degradation Mechanism ◽  
Autophagic Flux ◽  
Human Tissues ◽  
Tissue Analysis ◽  
Therapeutic Approaches ◽  
Tissue Samples ◽  
Clear Cut ◽  
Autophagic Activity ◽  
Made In

Autophagy is a highly conserved degradation mechanism that is essential for maintaining cellular homeostasis. In human disease, autophagy pathways are frequently deregulated and there is immense interest in targeting autophagy for therapeutic approaches. Accordingly, there is a need to determine autophagic activity in human tissues, an endeavor that is hampered by the fact that autophagy is characterized by the flux of substrates whereas histology informs only about amounts and localization of substrates and regulators at a single timepoint. Despite this challenging task, considerable progress in establishing markers of autophagy has been made in recent years. The importance of establishing clear-cut autophagy markers that can be used for tissue analysis cannot be underestimated. In this review, we attempt to summarize known techniques to quantify autophagy in human tissue and their drawbacks. Furthermore, we provide some recommendations that should be taken into consideration to improve the reliability and the interpretation of autophagy biomarkers in human tissue samples.

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