scholarly journals The potential role of long noncoding RNAs in primary open-angle glaucoma

2021 ◽  
Author(s):  
Feng Zhang ◽  
Yang Zhao ◽  
Mengdan Cao ◽  
Xu Jia ◽  
Zheng Pan ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Noncoding Rna ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Expression Profiles ◽  
Open Angle ◽  
Real Time Quantitative Pcr ◽  
Microarray Analyses ◽  
Normal Controls

Abstract Purpose To identify the potential genes in human trabecular meshwork (TM) related to primary open-angle glaucoma (POAG). Methods First, long noncoding RNA (LncRNA) and mRNA expression profiles in TM samples from 4 control subjects and 4 POAG patients were accessed by microarray analyses. Then, twenty lncRNAs were validated by real-time quantitative PCR in the same samples from microarray analyses. Finally, eight highly expressed lncRNAs were further tested by real-time quantitative PCR in TM from 8 normal controls and 19 POAG patients. Expression data were normalized and analyzed using the R software. Pathway analyses were performed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Results A total of 2179 lncRNAs and 923 mRNAs in the TM of POAG patients were significantly upregulated, and 3111 lncRNAs and 887 mRNAs were significantly downregulated. ENST00000552367, ENST00000582505, ENST00000609130, NR_029395, NR_038379, and ENST00000586949 expression levels were significantly higher in the TM from a different cohort of POAG patient than normal controls. Conclusion ENST00000552367, ENST00000582505, ENST000006091- 30, NR_029395, NR_038379, and ENST00000586949 may play essential roles in the development of POAG.

scholarly journals The Potential Role of Long Noncoding RNAs in Primary Open-Angle Glaucoma

2019 ◽  
Author(s):  
Feng Zhang ◽  
Yang Zhao ◽  
Mengdan Cao ◽  
Xu Jia ◽  
Zheng Pan ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Noncoding Rna ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Expression Profiles ◽  
Open Angle ◽  
Real Time Quantitative Pcr ◽  
Microarray Analyses ◽  
Normal Controls

Abstract Background To identify the potential genes in human trabecular meshwork (TM) related to primary open-angle glaucoma (POAG). At first, long noncoding RNA (LncRNA) and mRNA expression profiles in TM samples from 4 control subjects and POAG patients were accessed by microarray analyses. Then, twenty lncRNAs were validated by real-time quantitative PCR in the same samples from microarray analyses. Finally, eight highly expression lncRNAs were further tested by real-time quantitative PCR in TM from 8 normal controls and 19 POAG patients. Expression data were normalized and analyzed using the R software. Pathway analyses were performed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis.Results: A total of 2179 lncRNAs and 923 mRNAs in the TM of POAG patients were significantly up-regulated, and 3111 lncRNAs and 887 mRNAs were significantly down-regulated. ENST00000552367, ENST00000582505, ENST00000609130, NR_029395, NR_038379, and ENST00000586949 expression levels were significantly higher in the TM from a different cohort of POAG patient than normal controls.Conclusion: ENST00000552367, ENST00000582505, ENST000006091- 30, NR_029395, NR_038379, and ENST00000586949 may play essential roles in the development of POAG.

Circular RNA expression profile and m6A modification analysis in poorly differentiated adenocarcinoma of the stomach

Epigenomics ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1027-1040 ◽  
Author(s):  
Chenjing Zhang ◽  
Jingya Wang ◽  
Xiaoge Geng ◽  
Jiangfeng Tu ◽  
Huiqin Gao ◽  
...  
Keyword(s):  
Real Time ◽  
Expression Pattern ◽  
Quantitative Pcr ◽  
Expression Profiles ◽  
Area Under The Curve ◽  
Circular Rna ◽  
Rna Expression ◽  
Poorly Differentiated Adenocarcinoma ◽  
Real Time Quantitative Pcr ◽  
Poorly Differentiated

Aims: To profile and characterize the circular RNA (circRNA) expression pattern in poorly differentiated gastric adenocarcinoma (PDGA). Methods & materials: CircRNA expression profiles in PDGA and adjacent nontumor tissues were analyzed by microarray. Five randomly selected differentiated expressed circRNAs (DECs) were validated by real-time quantitative PCR. m6A qualification of the top 20 DECs was conducted by m6A-immunoprecipitation and real-time quantitative PCR. Results: A total of 65 DECs were found in PDGA compared with the control. Hsa_circRNA_0077837 had the largest area under the curve. Most DECs had m6A modifications, the trend of m6A modification alteration was mainly consistent with the circRNA expression level. Conclusion: Our study revealed a set of DECs and their m6A modification alterations, which may provide new insight for their potential function in PDGA.

Up-Regulation of Genes Involved in Folate Metabolism Characterize Late but Not Early Relapse in Childhood Acute Lymphoblastic Leukemia.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 1733-1733
Author(s):  
Laura Hogan ◽  
Deepa Bhojwani ◽  
Jinhua Wang ◽  
Debra Morrison ◽  
Jun J Yang ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Real Time ◽  
Quantitative Pcr ◽  
Expression Profiles ◽  
Lymphoblastic Leukemia ◽  
Nucleotide Metabolism ◽  
Clinical Samples ◽  
Late Relapse ◽  
Early Relapse ◽  
Real Time Quantitative Pcr

Abstract Abstract 1733 Poster Board I-759 Introduction Outcomes for relapsed pediatric acute lymphoblastic leukemia (ALL) are poor overall but depend on the timing of relapse, with lower survival rates for patients that relapse early (defined as < 36 months from diagnosis). Previously we sought to discover underlying biological pathways that mediate relapse by analyzing gene expression profiles in a large cohort of diagnosis/relapse paired bone marrow samples on the Affymetrix U133A arrays (Bhojwani et al, ASH 2008). We determined that early relapse was characterized by over-expression of cell cycle genes reflective of a proliferative state but late relapse was characterized by genes involved in nucleotide biosynthesis including targets of anti-folate agents. Given the potential therapeutic implications of these results we sought to validate these findings in an independent set of samples. Patients and Methods Validation of the first 60 pairs was performed using 26 additional pairs analyzed on Affymetrix U133Plus2 arrays. This validation set consisted of 17 early and 9 late diagnosis/relapse pairs. All patients had B precursor ALL and were at first relapse. Probesets differentially expressed between early and late relapse were identified in a supervised pair-wise analysis using an FDR cutoff of <10%. Expression of several genes found to be up-regulated at relapse by array expression were validated using real-time quantitative PCR. Results Evaluation of the last 26 pairs once again revealed distinct gene signatures that could distinguish between early and late relapse and many genes that were significant in the original 60 pairs were validated. Overall, thirty percent of the genes that distinguish diagnosis from relapse were validated in this smaller cohort. Importantly, genes involved in nucleotide metabolism that are targets of anti-folates such as thymidylate synthetase (TYMS), dihydrofolate reductase (DHFR) and methylenetetrahydrofolate dehydrogenase (MTHFD1) were validated as up-regulated at late relapse. TYMS was elevated at late relapse in the original (p=0.004) and validation (p=0.02) cohorts. MTHFD1 and DHFR also showed increased expression at late relapse (p=0.01 and p=0.09 for MTHFD1; and p=0.01and p=0.07 for DHFR) in both the discovery and validation cohorts respectively. Increased expression of DHFR and TYMS in late relapse was also confirmed by real-time quantitative PCR comparing 12 early and 12 late relapse pairs. Median expression at late relapse was 2 fold higher than diagnosis for both DHFR (p= 0.03) and TYMS (p= 0.01). Conclusion Increased expression of anti-folate target genes is seen in clinical samples from patients whose disease recurs late, indicating possible selection during maintenance where the bulk of anti-folate exposure occurs. Therapeutic strategies to augment folate antagonism may prevent late recurrences of ALL. Disclosures No relevant conflicts of interest to declare.

scholarly journals An evaluation of ocular elasticity using real-time ultrasound elastography in primary open-angle glaucoma

2016 ◽  
Vol 89 (1060) ◽  
pp. 20150429 ◽  
Author(s):  
Kadir Agladioglu ◽  
Gökhan Pekel ◽  
Seher Altintas Kasikci ◽  
Ramazan Yagci ◽  
Yilmaz Kiroglu
Keyword(s):  
Real Time ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Ultrasound Elastography ◽  
Open Angle

Real-Time Ultrasound Elastographic Features of Primary Open Angle Glaucoma

2016 ◽  
Vol 32 (4) ◽  
pp. 333-337 ◽  
Author(s):  
Ozlem Unal ◽  
Nurdan Cay ◽  
Fatma Yulek ◽  
Ayse Guzin Taslipinar ◽  
Selen Bozkurt ◽  
...  
Keyword(s):  
Real Time ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Open Angle

scholarly journals A new algorithm of treatment choice in primary open-angle glaucoma

2021 ◽  
Vol 14 (4) ◽  
pp. 7-17
Author(s):  
A. A. Antonov ◽  
I. V. Kozlova ◽  
A. A. Vitkov ◽  
T. M. Agadzhanyan
Keyword(s):  
Surgical Treatment ◽  
Real Time ◽  
Patient Management ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Ophthalmological Examination ◽  
Open Angle ◽  
Point Scale ◽  
Optimal Method ◽  
Hypotensive Therapy

Purpose: to develop a comprehensive algorithm for choosing the optimal method for monitoring and treating patients with primary openangle glaucoma (POAG). Material and methods. 30 patients with POAG, aged 30 to 86, underwent an ophthalmological examination which included visometry, standard automatic perimetry, tonometry, ophthalmoscopy, and biomicroscopy. The data obtained were evaluated on a point scale according to the innovative algorithm, on the basis of which a plan of patient management was recommended. Then the recommendation of the algorithm was compared with the suggestion of an expert specializing in the treatment of glaucoma. Results. The proposed innovative algorithm for treating POAG patients sets up four stages in the appointment of topical hypotensive therapy. When gaining a certain number of points, the algorithm recommends surgical treatment. The treatment tactics suggested by the algorithm, agreed with the opinion of glaucoma expert in most of the cases (27 out of 30 cases; 90%). Conclusion. An algorithm for the treatment of patients with POAG has been proposed and tested. The algorithm makes it possible to assess the progression of the glaucomatous process and select the optimal tactics for managing the patient in real time of examination.

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