Occurrence of cell surface arabinogalactan-protein and extensin epitopes in relation to pericycle and vascular tissue development in the root apex of four species

Planta ◽  
1998 ◽  
Vol 204 (2) ◽  
pp. 252-259 ◽  
Author(s):  
Pedro J. Casero ◽  
Ilda Casimiro ◽  
J. Paul Knox
Keyword(s):  
Cell Surface ◽  
Vascular Tissue ◽  
Root Apex ◽  
Arabinogalactan Protein ◽  
Tissue Development

scholarly journals Endothelial receptor-mediated binding of glucose-modified albumin is associated with increased monolayer permeability and modulation of cell surface coagulant properties.

1989 ◽  
Vol 170 (4) ◽  
pp. 1387-1407 ◽  
Author(s):  
C Esposito ◽  
H Gerlach ◽  
J Brett ◽  
D Stern ◽  
H Vlassara
Keyword(s):  
Endothelial Cell ◽  
Cell Surface ◽  
Vascular Tissue ◽  
Vascular Complications ◽  
Endothelial Permeability ◽  
Cell Surface Expression ◽  
Coagulation System ◽  
Advanced Glycosylation End Products ◽  
Cell Functions ◽  
Bsa Binding

Advanced glycosylation end products (AGE) of proteins accumulate in the vasculature with diabetes and aging, and are thought to be associated with vascular complications. This led us to examine the interaction of AGE-BSA as a prototype of this class of nonenzymatically glycosylated proteins subjected to further processing, with endothelium. Incubation of 125I-AGE-BSA with cultured bovine endothelium resulted in time-dependent, saturable binding that was half-maximal at a concentration of approximately 100 nM. Although unlabeled normal BSA was not a competitor, unlabeled AGE-BSA was an effective competitor of 125I-AGE-BSA-endothelial cell interaction. In addition, AGE modification of two alternative proteins, hemoglobin and ribonuclease, rendered them inhibitors of 125I-AGE-BSA binding to endothelium, although the native, unmodified forms of these proteins were not. At 37 degrees C, binding of 125I-AGE-BSA or gold-labeled AGE-BSA was followed by internalization and subsequent segregation either to a lysosomal compartment or to the endothelial-derived matrix after transcytosis. Exposure of endothelium to AGE-BSA led to perturbation of two important endothelial cell homeostatic properties, coagulant and barrier function. AGE-BSA downregulated the anticoagulant endothelial cofactor thrombomodulin, and induced synthesis and cell surface expression of the procoagulant cofactor tissue factor over the same range of concentrations that resulted in occupancy of cell surface AGE-BSA binding sites. In addition, AGE-BSA increased endothelial permeability, resulting in accelerated passage of an inert macromolecular tracer, [3H]inulin, across the monolayer. These results indicate that AGE derivatives of proteins, potentially important constituents of pathologic vascular tissue, bind to specific sites on the endothelial cell surface and modulate central endothelial cell functions. The interaction of AGE-modified proteins with endothelium may play an important role in the early stages of increased vascular permeability, as well as vessel wall-related abnormalities of the coagulation system, characteristic of diabetes and aging.

Production and transplantation of bioengineered lung into a large-animal model

2018 ◽  
Vol 10 (452) ◽  
pp. eaao3926 ◽  
Author(s):  
Joan E. Nichols ◽  
Saverio La Francesca ◽  
Jean A. Niles ◽  
Stephanie P. Vega ◽  
Lissenya B. Argueta ◽  
...  
Keyword(s):  
Vascular Tissue ◽  
Fundamental Problem ◽  
Transplant Rejection ◽  
Systemic Circulation ◽  
Large Animal Model ◽  
Large Animal ◽  
Bioreactor Culture ◽  
Tissue Development ◽  
Alveolar Tissue ◽  
Vessel Development

The inability to produce perfusable microvasculature networks capable of supporting tissue survival and of withstanding physiological pressures without leakage is a fundamental problem facing the field of tissue engineering. Microvasculature is critically important for production of bioengineered lung (BEL), which requires systemic circulation to support tissue survival and coordination of circulatory and respiratory systems to ensure proper gas exchange. To advance our understanding of vascularization after bioengineered organ transplantation, we produced and transplanted BEL without creation of a pulmonary artery anastomosis in a porcine model. A single pneumonectomy, performed 1 month before BEL implantation, provided the source of autologous cells used to bioengineer the organ on an acellular lung scaffold. During 30 days of bioreactor culture, we facilitated systemic vessel development using growth factor–loaded microparticles. We evaluated recipient survival, autograft (BEL) vascular and parenchymal tissue development, graft rejection, and microbiome reestablishment in autografted animals 10 hours, 2 weeks, 1 month, and 2 months after transplant. BEL became well vascularized as early as 2 weeks after transplant, and formation of alveolar tissue was observed in all animals (n= 4). There was no indication of transplant rejection. BEL continued to develop after transplant and did not require addition of exogenous growth factors to drive cell proliferation or lung and vascular tissue development. The sterile BEL was seeded and colonized by the bacterial community of the native lung.

scholarly journals Overexpression of MADS-box Gene AGAMOUS-LIKE 12 Activates Root Development in Juglans sp. and Arabidopsis thaliana

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 444
Author(s):  
Grégory Montiel ◽  
Muriel Gaudet ◽  
Françoise Laurans ◽  
Philippe Rozenberg ◽  
Matthieu Simon ◽  
...  
Keyword(s):  
Vascular Tissue ◽  
Root Apex ◽  
Abnormal Development ◽  
Mads Box ◽  
Mads Box Genes ◽  
Meristem Cell ◽  
Developmental Abnormalities ◽  
Wide Range ◽  
Transgenic Lines ◽  
Mads Box Gene

Until recently, the roles of plant MADS-box genes have mainly been characterized during inflorescence and flower differentiation. In order to precise the roles of AGAMOUS-LIKE 12, one of the few MADS-box genes preferentially expressed in roots, we placed its cDNA under the control of the double 35S CaMV promoter to produce transgenic walnut tree and Arabidopsis plants. In Juglans sp., transgenic somatic embryos showed significantly higher germination rates but abnormal development of their shoot apex prevented their conversion into plants. In addition, a wide range of developmental abnormalities corresponding to ectopic root-like structures affected the transgenic lines suggesting partial reorientations of the embryonic program toward root differentiation. In Arabidopsis, AtAGL12 overexpression lead to the production of faster growing plants presenting dramatically wider and shorter root phenotypes linked to increased meristematic cell numbers within the root apex. In the upper part of the roots, abnormal cell divisions patterns within the pericycle layer generated large ectopic cell masses that did not prevent plants to grow. Taken together, our results confirm in both species that AGL12 positively regulates root meristem cell division and promotes overall root vascular tissue formation. Genetic engineering of AGL12 expression levels could be useful to modulate root architecture and development.

scholarly journals The Histidine Kinases CYTOKININ-INDEPENDENT1 and ARABIDOPSIS HISTIDINE KINASE2 and 3 Regulate Vascular Tissue Development in Arabidopsis Shoots

2009 ◽  
Vol 21 (7) ◽  
pp. 2008-2021 ◽  
Author(s):  
Jan Hejátko ◽  
Hojin Ryu ◽  
Gyung-Tae Kim ◽  
Romana Dobešová ◽  
Sunhwa Choi ◽  
...  
Keyword(s):  
Vascular Tissue ◽  
Tissue Development ◽  
Histidine Kinases

Phenovariation induced in Streptocarpus prolixus (Gesneriaceae) by β-glucosyl Yariv reagent

2003 ◽  
Vol 81 (4) ◽  
pp. 338-344 ◽  
Author(s):  
Richard A Rauh ◽  
Dominick V Basile
Keyword(s):  
Pattern Formation ◽  
Cell Surface ◽  
Apical Meristem ◽  
Arabinogalactan Proteins ◽  
Arabinogalactan Protein ◽  
Pattern Change ◽  
Yariv Reagent ◽  
As Species ◽  
The Family ◽  
Primitive Species

Streptocarpus prolixus C.B. Clarke is a species in the family Gesneriaceae characterized by an acaulescent vegetative body. Instead of a stem bearing leaves developing from a shoot apical meristem, its vegetative body derives from the continued, intercalary growth of one of its cotyledons. β-glucosyl Yariv phenyglycoside (β-D-Glc)3 selectively binds a class of cell surface associated proteoglycans and glycoproteins known as arabinogalactan-proteins (AGPs). Treating seeds and seedlings of S. prolixus with (β-D-Glc)3 induced phenovariants considered phyogenetically significant because they copied morphological features (phenocopied) characteristic of other, presumably more primitive species of Streptocarpus as well as species in other genera in the family to which it belongs. These results paralleled those obtained in earlier experiments with S. prolixus using antimetabolites of hydroxyproline-containing proteins (Hyp-proteins). Treatment with α-galactosyl phenylglycoside, a Yariv reagent that does not bind AGPs, did not induce phenovariants. The finding that (β-D-Glc)3 produced the same "phyletic phenocopies" as Hyp-protein antagonists strongly suggests that the morphoregulatory Hyp-proteins that were antagonized in the earlier experiments were AGPs and that AGPs play a pivotal role in pattern formation and pattern change during plant morphogenesis.Key words: Yariv phenyglycoside, arabinogalactan-protein, AGPs, Streptocarpus, phenovariation, morphogenesis.

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