Detection of human papillomaviruses in squamous cell carcinomas of the lung

Squamous cell carcinomas (SCCs) in the anogenital and head and neck regions are associated with high-risk types of human papillomaviruses (HR-HPV). Deregulation of miRNA expression is an important contributor to carcinogenesis. This study aimed to pinpoint commonly and uniquely deregulated miRNAs in cervical, anal, vulvar, and tonsillar tumors of viral or non-viral etiology, searching for a common set of deregulated miRNAs linked to HPV-induced carcinogenesis. RNA was extracted from tumors and nonmalignant tissues from the same locations. The miRNA expression level was determined by next-generation sequencing. Differential expression of miRNAs was calculated, and the patterns of miRNA deregulation were compared between tumors. The total of deregulated miRNAs varied between tumors of different locations by two orders of magnitude, ranging from 1 to 282. The deregulated miRNA pool was largely tumor-specific. In tumors of the same location, a low proportion of miRNAs were exclusively deregulated and no deregulated miRNA was shared by all four types of HPV-positive tumors. The most significant overlap of deregulated miRNAs was found between tumors which differed in location and HPV status (HPV-positive cervical tumors vs. HPV-negative vulvar tumors). Our results imply that HPV infection does not elicit a conserved miRNA deregulation in SCCs.

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Retracted: Human Papillomaviruses and Squamous Cell Carcinomas of Head and Neck Region

Contemporary Oral Oncology ◽

10.1007/978-3-319-14911-0_3 ◽

2016 ◽

pp. 77-101

Author(s):

Priya R. Prabhu ◽

M. Radhakrishna Pillai

Keyword(s):

Head And Neck ◽

Squamous Cell ◽

Neck Region ◽

Squamous Cell Carcinomas ◽

Human Papillomaviruses ◽

Head And Neck Region

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High-Risk Human Papillomaviruses May Have an Important Role in Non–Oral Habits–Associated Oral Squamous Cell Carcinomas in Taiwan

American Journal of Clinical Pathology ◽

10.1309/c5p6nuq2nw6lctbp ◽

2003 ◽

Vol 120 (6) ◽

pp. 909-916 ◽

Cited By ~ 28

Author(s):

Julia Yu-Fong Chang ◽

Ming-Chieh Lin ◽

Chun-Pin Chiang

Keyword(s):

High Risk ◽

Squamous Cell ◽

Squamous Cell Carcinomas ◽

Human Papillomaviruses ◽

Oral Squamous Cell Carcinomas ◽

Oral Habits

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Causes and Consequences of HPV Integration in Head and Neck Squamous Cell Carcinomas: State of the Art

Cancers ◽

10.3390/cancers13164089 ◽

2021 ◽

Vol 13 (16) ◽

pp. 4089

Author(s):

Harini Balaji ◽

Imke Demers ◽

Nora Wuerdemann ◽

Julia Schrijnder ◽

Bernd Kremer ◽

...

Keyword(s):

Head And Neck ◽

Squamous Cell ◽

Methylation Status ◽

Squamous Cell Carcinomas ◽

Human Papillomaviruses ◽

Response To Therapy ◽

Detection Techniques ◽

Persistent Infections ◽

Viral Dna Integration ◽

The Impact

A constantly increasing incidence in high-risk Human Papillomaviruses (HPV)s driven head and neck squamous cell carcinomas (HNSCC)s, especially of oropharyngeal origin, is being observed. During persistent infections, viral DNA integration into the host genome may occur. Studies are examining if the physical status of the virus (episomal vs. integration) affects carcinogenesis and eventually has further-reaching consequences on disease progression and outcome. Here, we review the literature of the most recent five years focusing on the impact of HPV integration in HNSCCs, covering aspects of detection techniques used (from PCR up to NGS approaches), integration loci identified, and associations with genomic and clinical data. The consequences of HPV integration in the human genome, including the methylation status and deregulation of genes involved in cell signaling pathways, immune evasion, and response to therapy, are also summarized.

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