In vitro killing action of auranofin on Taenia crassiceps metacestode (cysticerci) and inactivation of thioredoxin–glutathione reductase (TGR)

Objetivo: Avaliar a atividade bioativa da desloratadina através de estudos in sílico por docagem molecular em organismos do gênero Schistosoma. Metodologia: Para o estudo foram coletadas proteínas em 3D dos alvos cathepsin B1 (2cb1), purine nucleoside phosphorylase (pnp), thioredoxin glutathione reductase (tgr), e uridine phosphorylase (up) no banco de dados Protein Data Bank (PDB). As análises de docagem foram realizadas pelo software Autodock Tools (ADT) versão ADT 1.5.6. Posteriormente o ligante desloratadina foi obtido no banco de dados PubChem em estrutura 2D e desenhado no programa GaussView 5.0 e otimizado pelo software Gaussian 09W em método Hartree-Fock na base Default Spin/3-21G. O restante dos parâmetros foi definido de acordo com o método padrão. Resultados: De todos os alvos analisados, a desloratadina obteve os melhores resultados nos receptores up e 2cb1 com energias de ligação de -8,49 e -8,35 Kcal.mol-1 respectivamente. Conclusão: Esses valores demonstram uma boa afinidade molecular em interação dos organismos com a droga desloratadina, além de terem resultados elevados de constante de inibição, demonstrando atividade bioativa da droga antiesquistossomose. Com os presentes resultados in sílico, a droga se torna uma alternativa em estudos bioativos antiesquistossomose, podendo dar continuidade em ensaios in vitro e ex vivo.

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Characterization and in vitro functional analysis of thioredoxin glutathione reductase from the liver fluke Opisthorchis viverrini

Acta Tropica ◽

10.1016/j.actatropica.2020.105621 ◽

2020 ◽

Vol 210 ◽

pp. 105621

Author(s):

Satya Prum ◽

Sirikanya Plumworasawat ◽

Sujittra Chaiyadet ◽

Prasert Saichua ◽

Raynoo Thanan ◽

...

Keyword(s):

Functional Analysis ◽

Glutathione Reductase ◽

Liver Fluke ◽

Opisthorchis Viverrini ◽

Thioredoxin Glutathione Reductase

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Correction: Effect of curcuminoids and curcumin derivate products on thioredoxin-glutathione reductase from Taenia crassiceps cysticerci. Evidence suggesting a curcumin oxidation product as a suitable inhibitor

PLoS ONE ◽

10.1371/journal.pone.0223795 ◽

2019 ◽

Vol 14 (10) ◽

pp. e0223795

Author(s):

Alberto Guevara-Flores ◽

José de Jesús Martínez-González ◽

Álvaro Miguel Herrera-Juárez ◽

Juan Luis Rendón ◽

Martín González-Andrade ◽

...

Keyword(s):

Glutathione Reductase ◽

Oxidation Product ◽

Taenia Crassiceps ◽

Correction Effect ◽

Thioredoxin Glutathione Reductase

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Mitochondrial Thioredoxin-Glutathione Reductase from LarvalTaenia crassiceps(Cysticerci)

Journal of Parasitology Research ◽

10.1155/2010/719856 ◽

2010 ◽

Vol 2010 ◽

pp. 1-11 ◽

Cited By ~ 7

Author(s):

Alberto Guevara-Flores ◽

Irene P. del Arenal ◽

Guillermo Mendoza-Hernández ◽

Juan Pablo Pardo ◽

Oscar Flores-Herrera ◽

...

Keyword(s):

Glutathione Reductase ◽

Reductase Activity ◽

Catalytic Efficiency ◽

Dependent Manner ◽

Exchange Reactions ◽

Disulfide Exchange ◽

Oxidative Inactivation ◽

Disulfide Reductase ◽

Thioredoxin Peroxidase ◽

Thioredoxin Glutathione Reductase

Mitochondrial thioredoxin-glutathione reductase was purified from larvalTaenia crassiceps(cysticerci). The preparation showed NADPH-dependent reductase activity with either thioredoxin or GSSG, and was able to perform thiol/disulfide exchange reactions. At25∘Cspecific activities were437 ± 27mU mg-1and840 ± 49mU mg-1with thioredoxin and GSSG, respectively. ApparentKmvalues were0.87 ± 0.04 μM,41 ± 6 μM and19 ± 10 μM for thioredoxin, GSSG and NADPH, respectively. Thioredoxin from eukaryotic sources was accepted as substrate. The enzyme reduced H2O2in a NADPH-dependent manner, although with low catalytic efficiency. In the presence of thioredoxin, mitochondrial TGR showed a thioredoxin peroxidase-like activity. All disulfide reductase activities were inhibited by auranofin, suggesting mTGR is dependent on selenocysteine. The reductase activity with GSSG showed a higher dependence on temperature as compared with the DTNB reductase activity. The variation of the GSSG- and DTNB reductase activities on pH was dependent on the disulfide substrate. Like the cytosolic isoform, mTGR showed a hysteretic kinetic behavior at moderate or high GSSG concentrations, but it was less sensitive to calcium. The enzyme was able to protect glutamine synthetase from oxidative inactivation, suggesting that mTGR is competent to contend with oxidative stress.

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Inhibition of in vitro and in vivo Mast Cell Degranulation by Taenia crassiceps Metacestodes in vitro Incubation Products

Zentralblatt für Bakteriologie ◽

10.1016/s0934-8840(89)80114-8 ◽

1989 ◽

Vol 271 (4) ◽

pp. 521-531 ◽

Cited By ~ 4

Author(s):

Birgit Seifert ◽

Egbert Geyer

Keyword(s):

Mast Cell ◽

Mast Cell Degranulation ◽

Taenia Crassiceps ◽

In Vitro Incubation

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Health benefits of new symbiotic “NAR”

Central Asian Journal of Global Health ◽

10.5195/cajgh.2013.114 ◽

2014 ◽

Vol 2 ◽

Author(s):

Saule Saduakhasova ◽

Almagul Kushugulova ◽

Samat Kozhakhmetov ◽

Gulnara Shakhabayeva ◽

Adil Supiyev ◽

...

Keyword(s):

Antioxidant Activity ◽

Superoxide Dismutase ◽

Glutathione Reductase ◽

Inflammatory Mediators ◽

Antioxidant Activities ◽

Reductase Activity ◽

Protein A ◽

Intact Cells ◽

Total Antioxidant

Introduction: The immune-modulatory effects of synbiotics and their ability to reduce free radical levels may be useful for functional food that is able to be active throughout whole period of colonization of the gastrointestinal tract.The aim of the present study was to investigate the immune-modulatory and antioxidant effects of the synbiotic product "NАR," a probiotic beverage.Methods: The presence of IL-2, IL-4, IL-6, IL-8, IL-10, αTNF, γIFN, Ig A, Ig M, and Ig E was studied in vitro using a solid immunosorbent analysis. The total antioxidant activities of superoxide dismutase and glutathione reductase were determined by a spectrophotometry using the Sigma-Aldrich sets.Results: Studies of the immune-modulatory properties of the synbiotic product NAR showed 1.7 fold increase of γINF levels (p<0.01) in blood after consumption of the synbiotic product “NAR” in comparison to control values, whereas the concentrations of IL-4 and Ig E decreased 2.0 times (treatment: 9.3; control: 18.7; p<0.01) and 1.3 times (p<0.1), respectively. The consumption of the synbiotic product “NAR” caused an increase in the proportion of γINF/IL 4 (treatment: 15.4; control: 4.4; p<0.01), which indicates a reduction in functional activity of Th2-type lymphocytes in comparison with the function of Th1 cells.Our study showed a high level of the total antioxidant activity of the synbiotic product (67.4 mmol/ml). The antioxidant activity of the intact cells of consortium (15.3 mM/ml), which was the basis for the preparation of the symbiotic product, is several times lower than the activity observed in the symbiotic samples.Expression of SOD is one of the mechanisms of antioxidant stress radicals inactivation by bacteria. The analysis identified a superoxide dismutase activity of synbiotic product (1.42 U/mg protein). A glutathione reductase activity of the synbiotic product was elevated (0.06 U/ml). Conclusion: The majority of the inflammatory mediators found in the blood after the consumption of symbiotic product NAR were inflammatory mediators that activate a cellular component of the resistance. Moreover, the symbiotic product has a high antioxidant activity.

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