Inhibition of in vitro and in vivo Mast Cell Degranulation by Taenia crassiceps Metacestodes in vitro Incubation Products

1989 ◽  
Vol 271 (4) ◽  
pp. 521-531 ◽  
Author(s):  
Birgit Seifert ◽  
Egbert Geyer
Keyword(s):  
Mast Cell ◽  
Mast Cell Degranulation ◽  
Taenia Crassiceps ◽  
In Vitro Incubation

scholarly journals SLC10A4 regulates IgE-mediated mast cell degranulation in vitro and mast cell-mediated reactions in vivo

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Hanna Pettersson ◽  
Behdad Zarnegar ◽  
Annika Westin ◽  
Viktor Persson ◽  
Christiane Peuckert ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cell Degranulation ◽  
Ige Mediated

scholarly journals Glaucocalyxin A Attenuates Allergic Responses by Inhibiting Mast Cell Degranulation through p38MAPK/NrF2/HO-1 and HMGB1/TLR4/NF-κB Signaling Pathways

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Yihua Piao ◽  
Jingzhi Jiang ◽  
Zhiguang Wang ◽  
Chongyang Wang ◽  
Shan Jin ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Signaling Pathway ◽  
Nuclear Translocation ◽  
Calcium Influx ◽  
Mast Cell Degranulation ◽  
Therapeutic Drug ◽  
High Mobility Group Protein

Glaucocalyxin A (GLA) has various pharmacological effects like antioxidation, immune regulation, and antiatherosclerosis. Here, in this study, the effect and mechanism of GLA on mast cell degranulation were studied. The results of the anti-DNP IgE-mediated passive cutaneous anaphylaxis (PCA) showed that GLA dramatically inhibited PCA in vivo, as evidenced by reduced Evans blue extravasation and decreased ear thickness. In addition, GLA significantly reduced the release of histamine and β-hexosaminidase, calcium influx, cytokine (IL-4, TNF-α, IL-1β, IL-13, and IL-8) production in the RBL-2H3 (rat basophilic leukemia cells), and RPMCs (peritoneal mast cells) in vitro. Moreover, we further investigated the regulatory mechanism of GLA on antigen-induced mast cells by Western blot, which showed that GLA inhibited FcεRI-mediated signal transduction and invalidated the phosphorylation of Syk, Fyn, Lyn, Gab2, and PLC-γ1. In addition, GLA inhibited the recombinant mouse high mobility group protein B1- (HMGB1-) induced mast cell degranulation through limiting nuclear translocation of NF-κBp65. Treatment of mast cells with siRNA-HMGB1 significantly inhibited HMGB1 levels, as well as MyD88 and TLR4, decreased intracellular calcium levels, and suppressed the release of β-hexosaminidase. Meanwhile, GLA increased NrF2 and HO-1 levels by activating p38MAPK phosphorylation. Consequently, these data suggest that GLA regulates the NrF2/HO-1 signaling pathway through p38MAPK phosphorylation and inhibits HMGB1/TLR4/NF-κB signaling pathway to reduce mast cell degranulation and allergic inflammation. Our findings could be used as a promising therapeutic drug against allergic inflammatory disease.

In vitro and in vivo Inhibition of Mast Cell Degranulation by a Factor from Schistosoma mansoni

1980 ◽  
Vol 63 (2) ◽  
pp. 178-189 ◽  
Author(s):  
Christine Mazingue ◽  
Daniel Camus ◽  
Jean-Paul Dessaint ◽  
Monique Capron ◽  
André Capron
Keyword(s):  
Mast Cell ◽  
Schistosoma Mansoni ◽  
Mast Cell Degranulation

In vitro activation of murine DRG neurons by CGRP-mediated mucosal mast cell degranulation

2004 ◽  
Vol 287 (1) ◽  
pp. G178-G191 ◽  
Author(s):  
F. De Jonge ◽  
A. De Laet ◽  
L. Van Nassauw ◽  
J. K. Brown ◽  
H. R. P. Miller ◽  
...  
Keyword(s):  
Mast Cell ◽  
Nerve Fibers ◽  
Mast Cell Degranulation ◽  
Mucosal Mast Cell ◽  
Close Apposition ◽  
Drg Neurons ◽  
Nodose Ganglia ◽  
Mouse Mast Cell

Upregulation of CGRP-immunoreactive (IR) primary afferent nerve fibers accompanied by mastocytosis is characteristic for the Schistosoma mansoni-infected murine ileum. These mucosal mast cells (MMC) and CGRP-IR fibers, which originate from dorsal root (DRG) and nodose ganglia, are found in close apposition. We examined interactions between primary cultured MMC and CGRP-IR DRG neurons in vitro by confocal recording of intracellular Ca2+concentration ([Ca2+]i). The degranulatory EC50for the mast cell secretagogue compound 48/80 (C48/80; 10 μg/ml) and the neuropeptides CGRP (2.10−8M) and substance P (SP; 3.10−8M) were determined by measurement of extracellular release of the granule chymase, mouse mast cell protease-1. Application of C48/80 (10 μg/ml) and CGRP and SP (both 10−7M) to Fluo-4-loaded MMC induced a transient rise in [Ca2+]iafter a lag time, indicative of mast cell degranulation and/or secretion. The CGRP response could be completely blocked by pertussis toxin (2 μg/ml), indicating involvement of Giproteins. Application of MMC juice, obtained by C48/80 degranulation of MMC, to Fluo-4-loaded DRG neurons induced in all neurons a rise in [Ca2+]i, indicative of activation. Degranulation of MMC by C48/80 in culture dishes containing Fluo-4-loaded DRG neurons also caused activation of the DRG neurons. In conclusion, these results demonstrate a bidirectional cross-talk between cultured MMC and CGRP-IR DRG neurons in vitro. This indicates that such a communication may be the functional relevance for the close apposition between MMC and CGRP-IR nerve fibers in vivo.

scholarly journals Allergic Airway-Induced Hypersensitivity Is Attenuated by Bergapten in Murine Models of Inflammation

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Douglas B. Aidoo ◽  
David D. Obiri ◽  
Newman Osafo ◽  
Aaron O. Antwi ◽  
Leslie B. Essel ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mast Cell ◽  
Protein Concentration ◽  
Allergic Inflammation ◽  
Neutrophil Infiltration ◽  
Lavage Fluid ◽  
Mast Cell Degranulation ◽  
Murine Models

Bergapten (5-methoxypsoralen, 5-MOP) is a plant-derived furocoumarin with demonstrated anti-inflammatory action. The present study investigated its effects on allergic inflammation in two related pathways of mast cell degranulation. Compound 48/80 and lipopolysaccharide (LPS) were used to activate the IgE-independent pathway while bovine serum albumin (BSA) was used as allergen for the IgE-dependent pathway. The modulatory effect of bergapten on mast cell degranulation, neutrophil extravasation, protein concentration, lung histopathology, and oxidative stress was assessed. Bergapten at 10, 30, and 100 μg/ml for 15 min stabilized mast cells in rat mesenteric tissue from disruption in vitro and when administered in vivo at 3, 10, and 30 mg kg−1 for 1 h protected mice from fatal anaphylaxis induced by compound 48/80. Similarly, treatment of LPS-challenged mice with bergapten (3, 10, and 30 mg kg−1) for 24 h significantly decreased neutrophil infiltration into bronchoalveolar lavage fluid, mean protein concentration, and inflammatory cell infiltration of pulmonary tissues when compared to the saline-treated LPS-challenged control. In addition, lung histology of the bergapten-treated LPS-challenged mice showed significantly less oedema, congestion, and alveolar septa thickening when compared to the saline-treated LPS-challenged disease control. LPS-induced oxidative stress was significantly reduced through increased tissue activities of catalase and superoxide dismutase and reduced malondialdehyde levels on treatment with bergapten. In the triple antigen-induced active anaphylaxis, daily administration of bergapten at 3, 10, and 30 mg kg−1 for 10 days, respectively, protected previously sensitized and challenged mice against anaphylactic shock. Overall, our study demonstrates the ability of bergapten to attenuate allergic airway-induced hypersensitivity in murine models of inflammation, suggesting its possible therapeutic benefit in this condition.

scholarly journals ELKS1 controls mast cell degranulation by regulating the transcription of Stxbp2 and Syntaxin 4 via Kdm2b stabilization

2020 ◽  
Vol 6 (31) ◽  
pp. eabb2497
Author(s):  
Hiu Yan Lam ◽  
Surendar Arumugam ◽  
Han Gyu Bae ◽  
Cheng Chun Wang ◽  
Sangyong Jung ◽  
...  
Keyword(s):  
Mast Cell ◽  
Cytokine Production ◽  
Nuclear Factor Κb ◽  
Mast Cell Degranulation ◽  
Nuclear Factor ◽  
Regulated Exocytosis ◽  
Lysine Specific Demethylase ◽  
Syntaxin 4

ELKS1 is a protein with proposed roles in regulated exocytosis in neurons and nuclear factor κB (NF-κB) signaling in cancer cells. However, how these two potential roles come together under physiological settings remain unknown. Since both regulated exocytosis and NF-κB signaling are determinants of mast cell (MC) functions, we generated mice lacking ELKS1 in connective tissue MCs (Elks1f/f Mcpt5-Cre) and found that while ELKS1 is dispensable for NF-κB–mediated cytokine production, it is essential for MC degranulation both in vivo and in vitro. Impaired degranulation was caused by reduced transcription of Syntaxin 4 (STX4) and Syntaxin binding protein 2 (Stxpb2), resulting from a lack of ELKS1-mediated stabilization of lysine-specific demethylase 2B (Kdm2b), which is an essential regulator of STX4 and Stxbp2 transcription. These results suggest a transcriptional role for active-zone proteins like ELKS1 and suggest that they may regulate exocytosis through a novel mechanism involving transcription of key exocytosis proteins.

scholarly journals Real-time imaging of mast cell degranulation in vitro and in vivo

2016 ◽  
Vol 479 (3) ◽  
pp. 517-522 ◽  
Author(s):  
Kayo Horiguchi ◽  
Soichiro Yoshikawa ◽  
Asuka Saito ◽  
Salma Haddad ◽  
Takuya Ohta ◽  
...  
Keyword(s):  
Mast Cell ◽  
Real Time ◽  
Mast Cell Degranulation ◽  
Real Time Imaging

Mitochondria-targeted antioxidant SkQ1 (10-(6′-plastoquinonyl)decyltriphenylphosphonium bromide) inhibits mast cell degranulation in vivo and in vitro

2017 ◽  
Vol 82 (12) ◽  
pp. 1493-1503 ◽  
Author(s):  
M. A. Chelombitko ◽  
O. A. Averina ◽  
T. V. Vasilyeva ◽  
O. Yu. Pletiushkina ◽  
E. N. Popova ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cell Degranulation

scholarly journals Docosahexaenoyl ethanolamide mitigates IgE-mediated allergic reactions by inhibiting mast cell degranulation and regulating allergy-related immune cells

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Kosuke Nishi ◽  
Yoshiki Kanayama ◽  
In-Hae Kim ◽  
Akihiro Nakata ◽  
Hisashi Nishiwaki ◽  
...  
Keyword(s):  
Mast Cell ◽  
Immune Cells ◽  
Mast Cell Degranulation ◽  
Allergic Symptom ◽  
Chain Polyunsaturated Fatty Acid ◽  
Dependent Manner ◽  
Ige Mediated ◽  
Dose Dependent

Abstract Docosahexaenoic acid (DHA) is a long-chain polyunsaturated fatty acid mainly found in fish oil. Although several studies have suggested that it can alleviate allergy symptoms, its mechanism of action remains to be elucidated. In the present study, we found that docosahexaenoyl ethanolamide (DHEA), a metabolite of DHA produced in the human body, exerts the anti-allergic activity in vitro and in vivo. DHEA suppressed degranulation of rat basophilic leukemia RBL-2H3 cells and bone marrow-derived mast cells in a dose-dependent manner without cytotoxicity. This occurred due to a decrease in Ca2+ influx, which is critical for mast cell degranulation. DHEA also suppressed IgE-mediated passive cutaneous anaphylaxis reaction in mice. In addition, DHEA was demonstrated to lessen an allergic symptom in a mouse model of pollinosis and to alter the production of IgE and cytokines secreted by splenocytes collected from the pollinosis mice. Taken together, this study indicates that DHEA is a promising anti-allergic agent as it inhibits mast cell degranulation and modulates other immune cells.

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