In vitro influence of temperature on the biological control activity of the fungus Duddingtonia flagrans against Haemonchus contortus in sheep

2012 ◽  
Vol 112 (2) ◽  
pp. 473-478 ◽  
Author(s):  
Rodrigo Buske ◽  
Janio Morais Santurio ◽  
Clarissa Vasconcelos de Oliveira ◽  
Liziane Aita Bianchini ◽  
José Henrique Souza da Silva ◽  
...  
2005 ◽  
Vol 79 (4) ◽  
pp. 315-319 ◽  
Author(s):  
J.B. Chauhan ◽  
P.K. Sanyal ◽  
R.B. Subramanian

AbstractAn in vitro study was carried out to determine efficacy of Indian isolates of the nematode-trapping fungi Arthrobotrys musiformis and Duddingtonia flagrans to capture infective larvae of Haemonchus contortus. These fungi have previously been screened and selected for their survival in the gastrointestinal tract of sheep without losing growth and nematode capturing potential. Following the feeding of chlamydospores of these two fungi alone or in combination in sheep experimentally infected with Haemonchus contortus, coprocultures were set up to enumerate the infective third stage larvae. The number of larvae captured from faeces of fungus-fed sheep was significantly higher compared with fungus-unfed controls irrespective of the fungus used. The fungal combination produced no antagonistic effect and thus can be used as efficiently as the fungi alone in the biological control of animal parasitic nematodes.


Author(s):  
Castañeda Alvarez Estefania ◽  
Sánchez Leal Ligia

For farmers the use of agrochemicals is the preferred method to control pests and diseases. Considering the market demand for biological control products, the encapsulation could be a competent alternative to current commercial formulations for cellular viability and controlled release. The purpose of this study was to use ionic gelation with sodium alginate, starch and maltodextrin to immobilize Bacillus subtilis and to evaluate the biocontrol effect against Fusarium oxysporum f. sp. lycopersici in vitro. The matrix with a concentration of 2% sodium alginate, 1% starch, and 1% maltodextrin is a suitable method for cellular viability and biological control activity against Fusarium oxysporum f. sp. lycopersici, with a reduction of mycelial growth of 49.6% and a survival rate for Bacillus subtilis of 98.05% (p less than 0.0001).The use of immobilized bacteria as biological control agents are sustainable and effective bio-inputs that could be used at industrial scale and benefit the tomato crops against attack by Fusarium oxysporum f. sp. lycopersici.


3 Biotech ◽  
2020 ◽  
Vol 10 (2) ◽  
Author(s):  
Fábio Ribeiro Braga ◽  
Carolina Magri Ferraz ◽  
Edir Nepomuceno da Silva ◽  
Jackson Victor de Araújo

1999 ◽  
Vol 50 (8) ◽  
pp. 1469 ◽  
Author(s):  
S. Simpfendorfer ◽  
T. J. Harden ◽  
G. M. Murray

The interaction between 29 isolates of Rhizobium and the in vitro growth of 3 strains of Phytophthora clandestina was investigated to determine the potential of these bacteria as biological control agents against root rot of subterranean clover (Trifolium subterraneum L.). The biological control activity of Rhizobium on the severity of root disease in seedlings was also investigated under glasshouse conditions. Thirteen of the 29 Rhizobium isolates caused significant reductions in the hyphal growth of the 3 P. clandestina isolates examined. Inoculation of seedlings with Rhizobium trifolii reduced the severity of root disease by 14–58% with corresponding increases in dry matter production of 20–73%. These results indicate that Rhizobium species have potential as biological control agents against the root rot of T. subterraneum seedlings caused by P. clandestina.


1990 ◽  
Vol 4 (6) ◽  
pp. 704-706 ◽  
Author(s):  
J.A. Melendez ◽  
J.G. Stone ◽  
E. Delphin ◽  
C.Y. Quon

1977 ◽  
Vol 55 (22) ◽  
pp. 2832-2837 ◽  
Author(s):  
R. C. Pearson ◽  
H. S. Aldwinckle ◽  
R. C. Seem

The influence of temperature on teliospore germination and basidiospore formation in Gymnosporangium juniperi-virginianae was studied in vitro. Teliospores germinated from 8 to 30 °C. Germination was first observed after 2 h from 12–28 °C but longer periods were required at lower temperatures. Promycelia formed basidiospores by 4 h from 12 to 24 °C but not until 7 h at 8 °C. Abortive germination of teliospores, without production of basidiospores, occurred from 26 to 30 °C. No germination was observed from 2 to 6 °C. Multiple regression analyses were performed and an equation relating germination of teliospores to temperature and time was formulated.


1998 ◽  
Vol 72 (4) ◽  
pp. 343-347 ◽  
Author(s):  
P. Mendoza de Gives ◽  
J. Flores Crespo ◽  
D. Herrera Rodriguez ◽  
V. Vazquez Prats ◽  
E. Liebano Hernandez ◽  
...  

AbstractA single oral dose of an aqueous suspension containing 11,350,000 chlamydospores of a Mexican isolate of Duddingtonia flagrans (FTHO-8) given to sheep, resulted in a maximum reduction of 88% (range 86.7–90.4%) of the population of Haemonchus contortus infective larvae in the faeces. The effect of this treatment continued for 4–5 days after administration of the suspension. The possible use of this treatment as a method of control of ovine haemonchosis is discussed.


Sign in / Sign up

Export Citation Format

Share Document