Differential diagnosis of cystic and alveolar echinococcosis using an immunochromatographic test based on the detection of specific antibodies

2013 ◽  
Vol 112 (10) ◽  
pp. 3627-3633 ◽  
Author(s):  
Jun-yun Wang ◽  
Chun-hua Gao ◽  
Dietmar Steverding ◽  
Xia Wang ◽  
Feng Shi ◽  
...  
2006 ◽  
Vol 13 (5) ◽  
pp. 553-555 ◽  
Author(s):  
Xiaohong Huang ◽  
Xuenan Xuan ◽  
Rodolfo A. Verdida ◽  
Shoufa Zhang ◽  
Naoaki Yokoyama ◽  
...  

ABSTRACT An immunochromatographic test for the simultaneous detection of Babesia caballi- and B. equi-specific antibodies (BceICT) was developed using a recombinant B. caballi 48-kDa rhoptry protein (rBc48) and a recombinant truncated B. equi merozoite antigen 2 (rEMA-2t). An evaluation of the ability of the BceICT to detect antibodies in sera from uninfected horses and experimentally infected horses showed high sensitivities and specificities of 83.3% (10/12 sera) and 92.9% (52/56 sera), respectively, for the anti-B. caballi antibody and 94.1% (16/17 sera) and 88.2% (45/51 sera), respectively, for the anti-B. equi antibody. Results from the detection of antibodies in field-collected sera indicated that the BceICT results corresponded with those of enzyme-linked immunosorbent assays (ELISA), showing 91.8% correspondence (67/73 sera) for B. caballi and 95.9% correspondence (70/73 sera) for B. equi, and that the BceICT results also corresponded with the ICT for B. caballi and for B. equi, both of which were 98.2% (55/56 sera). The comparable results of the ICT and ELISA and the simplicity and rapidity of the performance of the ICT suggest that the BceICT would be a feasible test for the simultaneous serodiagnosis of both agents of equine babesiosis in the field.


2019 ◽  
Vol 208 (6) ◽  
pp. 773-780 ◽  
Author(s):  
Hossein Hashemi ◽  
Jaleh Varshosaz ◽  
Hossein Fazeli ◽  
Seyedeh Maryam Sharafi ◽  
Hossein Mirhendi ◽  
...  

2020 ◽  
Vol 44 (3) ◽  
pp. 179-181
Author(s):  
Burcu Saka ◽  
Aslı Ünlü Akhan ◽  
Cengiz Erol ◽  
Ayşe İstanbullu Tosun ◽  
Gökhan Ertuğrul

2020 ◽  
Vol 65 (11) ◽  
pp. 688-692
Author(s):  
Yulia Aleksandrovna Akinshina ◽  
S. S. Mardanly ◽  
V. A. Kiseleva

The study presents the results of the creation and evaluation of the diagnostic characteristics of the rapid immunochromatographic test for the qualitative detection and differentiation of IgM/IgG antibodies to SARS-CoV-2 in human serum, plasma, and whole blood “ИХА-COVID-19-IgM / IgG”. Have been tested some samples without antibodies to SARS-CoV-2 and a samples with two and one type of specific antibodies. The coincidence of the results of immunochromatographic analysis with the results of the immunochemiluminescent method was 87.2%. Test kit can be use as the rapid diagnostic test in the context of the COVID-19 pandemic and to assess the immune status of convalescents.


2020 ◽  
Vol 10 (3) ◽  
pp. 738 ◽  
Author(s):  
Dmitriy V. Sotnikov ◽  
Lyubov V. Barshevskaya ◽  
Anatoly V. Zherdev ◽  
Saule Z. Eskendirova ◽  
Kassym K. Mukanov ◽  
...  

In this article, we describe an immunochromatographic test system developed for rapid serodiagnostics of cattle brucellosis using two markers: Gold nanoparticles (GNPs) and quantum dots (QDs). The test system was compared with immunochromatographic serodiagnostics systems that use only one marker. The approbation of the test system was conducted on samples of cattle sera with low, but diagnostically significant titers of specific antibodies. We show that when two conjugates are used, the intensity of the detectable signal increases by 2–3 times compared with the test system using the QD conjugate and by more than nine times compared with the system using the GNP conjugate.


2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Chun-hua Gao ◽  
Jun-yun Wang ◽  
Feng Shi ◽  
Dietmar Steverding ◽  
Xia Wang ◽  
...  

2021 ◽  
Vol 58 (1) ◽  
pp. 100-105
Author(s):  
K. Šimeková ◽  
R. Rosoľanka ◽  
M. Szilágyová ◽  
D. Antolová ◽  
E. Nováková ◽  
...  

SummaryHuman alveolar echinococcosis (AE) is a silently-progressing disorder that has become a threat in many countries. Since 2000, when the first case was recorded, the number of human AE patients in Slovakia is on continuous raise. The article presents a rare case of alveolar echinococcosis with infiltration in the adrenal gland and discusses the problems associated with differential diagnosis of the disease. In 2016, abdominal ultrasound performed due abdominal pain complaint showed the presence of cystic lesions in the right liver lobe of 54-year old female patient. During surgery, another lesion in the right adrenal gland was found, and neoplastic processes or echinococcosis were considered in the differential diagnosis. Due to unclear correlation between radiology, serology and histopathology results and endemic situation in Slovakia, molecular examination was recommended. Subsequently E. multilocularis was confirmed as etiological agent of infection. Alveolar echinococcosis is considered as a rare disease, with very few patients referred to clinicians or hospitals that sometimes have almost none existing experience with the diagnosis and treatment of the disease. Therefore, the establishment of networks or reference centres specialized on management of the disease would be suitable way to provide the patients with the best care and improve the disease diagnosis, treatment and prognosis.


1998 ◽  
Vol 34 (4) ◽  
pp. 309-314 ◽  
Author(s):  
M Haller ◽  
P Deplazes ◽  
F Guscetti ◽  
JC Sardinas ◽  
I Reichler ◽  
...  

Surgical removal of macroscopically detectable metacestode tissue followed by postoperative chemotherapy according to established human protocols resulted in complete clinical remission and immediate normalization of hyperglobulinemia in a dog with alveolar echinococcosis (AE). The disease is caused by the metacestode stage of the cestode, Echinococcus multilocularis. In endemic areas, AE should be included in the differential diagnosis of polycystic liver masses, especially if concomitant hyperglobulinemia is present. However, the importance of AE is not only the disease of the single dog itself but also the potential risk of infection for humans in an endemic area.


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