Morphogenesis and proliferation in mono- and organotypic co-cultures of primary human periodontal ligament fibroblasts and alveolar bone cells

Cultures of human periodontal ligament fibroblasts (HPLF) and human alveolar bone cells (HABC) were established, maintained, and characterized with respect to alkaline phosphatase (ALPase) activity, a marker enzyme of bone cells. During the proliferative phase, ALPase of HPLF and HABC were not detectable. But at the confluent stage, HPLF synthesized levels of ALPase similar to HABC. Also, ALPase activity of HPLF was markedly stimulated (7.5-fold) by 5 x 10-9 mol/L 1,25 (OH)2-vitamin D3 (1,25 (OH)2-D3). Therefore, since ALPase activity of HPLF resembles that present in osteoblasts, human periodontal ligament fibroblasts may function as "osteoblastic" fibroblasts.

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Effect of Interleukin-1β on Transforming Growth Factor-Beta and Bone Morphogenetic Protein-2 Expression in Human Periodontal Ligament and Alveolar Bone Cells in Culture: Modulation by Avocado and Soybean Unsaponifiables

Journal of Periodontology ◽

10.1902/jop.2006.050356 ◽

2006 ◽

Vol 77 (7) ◽

pp. 1156-1166 ◽

Cited By ~ 30

Author(s):

R. Andriamanalijaona ◽

H. Benateau ◽

P.E. Barre ◽

K. Boumediene ◽

D. Labbe ◽

...

Keyword(s):

Growth Factor ◽

Transforming Growth Factor Beta ◽

Periodontal Ligament ◽

Transforming Growth Factor ◽

Alveolar Bone ◽

Bone Cells ◽

Bone Morphogenetic Protein 2 ◽

Morphogenetic Protein ◽

Growth Factor Beta ◽

Alveolar Bone Cells

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Influence of static forces on the expression of selected parameters of inflammation in periodontal ligament cells and alveolar bone cells in a co-culture in vitro model

Clinical Oral Investigations ◽

10.1007/s00784-018-2697-2 ◽

2018 ◽

Vol 23 (6) ◽

pp. 2617-2628 ◽

Cited By ~ 5

Author(s):

Jianwei Shi ◽

Uwe Baumert ◽

Matthias Folwaczny ◽

Andrea Wichelhaus

Keyword(s):

Periodontal Ligament ◽

In Vitro Model ◽

Alveolar Bone ◽

Bone Cells ◽

Periodontal Ligament Cells ◽

Culture In Vitro ◽

Vitro Model ◽

Alveolar Bone Cells

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Proteomic assessment of human, periodontal ligament fibroblasts, gingival fibroblasts and alveolar bone osteoblasts

Australian Dental Journal ◽

10.1111/j.1834-7819.2007.tb06146.x ◽

2007 ◽

Vol 52 (S4) ◽

pp. S38-S38

Author(s):

PS Zilm ◽

PM Bartold ◽

V Marino

Keyword(s):

Periodontal Ligament ◽

Alveolar Bone ◽

Gingival Fibroblasts ◽

Periodontal Ligament Fibroblasts ◽

Human Periodontal Ligament Fibroblasts

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Although Anatomically Micrometers Apart: Human Periodontal Ligament Cells Are Slightly More Active in Bone Remodeling Than Alveolar Bone Derived Cells

Frontiers in Cell and Developmental Biology ◽

10.3389/fcell.2021.709408 ◽

2021 ◽

Vol 9 ◽

Author(s):

Rebecca Loo-Kirana ◽

Marjolijn Gilijamse ◽

Jolanda Hogervorst ◽

Ton Schoenmaker ◽

Teun J. de Vries

Keyword(s):

Bone Remodeling ◽

Periodontal Ligament ◽

Alveolar Bone ◽

Bone Cells ◽

Complex Structure ◽

Total Duration ◽

Alizarin Red ◽

Pdl Cells ◽

Alveolar Bone Cells

The periodontal ligament (PDL) and the alveolar bone are part of the periodontium, a complex structure that supports the teeth. The alveolar bone is continuously remodeled and is greatly affected by several complex oral events, like tooth extraction, orthodontic movement, and periodontitis. Until now, the role of PDL cells in terms of osteogenesis and osteoclastogenesis has been widely studied, whereas surprisingly little is known about the bone remodeling capacity of alveolar bone. Therefore, the purpose of this study was to compare the biological character of human alveolar bone cells and PDL cells in terms of osteogenesis and osteoclastogenesis in vitro. Paired samples of PDL cells and alveolar bone cells from seven patients with compromised general and oral health were collected and cultured. Bone A (early outgrowth) and bone B (late outgrowth) were included. PDL, bone A, bone B cell cultures all had a fibroblast appearance with similar expression pattern of six mesenchymal markers. These cultures were subjected to osteogenesis and osteoclastogenesis assays. For osteoclastogenesis assays, the cells were co-cultured with peripheral blood mononuclear cells, a source for osteoclast precursor cells. The total duration of the experiments was 21 days. Osteogenesis was slightly favored for PDL compared to bone A and B as shown by stronger Alizarin red staining and higher expression of RUNX2 and Collagen I at day 7 and for ALP at day 21. PDL induced approximately two times more osteoclasts than alveolar bone cells. In line with these findings was the higher expression of cell fusion marker DC-STAMP in PDL-PBMC co-cultures compared to bone B at day 21. In conclusion, alveolar bone contains remodeling activity, but to a different extent compared to PDL cells. We showed that human alveolar bone cells can be used as an in vitro model to study bone remodeling.

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08 / Expression and function of macrophage erythroblast attacher in THP-1 cells and human periodontal ligament fibroblasts stimulated with Porphyromonas gingivalis derived lipopolysaccharide

10.26226/morressier.5ac383252afeeb00097a3ae7 ◽

2018 ◽

Author(s):

Yulan Che

Keyword(s):

Porphyromonas Gingivalis ◽

Periodontal Ligament ◽

Periodontal Ligament Fibroblasts ◽

Human Periodontal Ligament Fibroblasts ◽

And Function

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Bone Invasive Properties of Oral Squamous Cell Carcinoma and its Interactions with Alveolar Bone Cells: An In Vitro Study

Current Cancer Drug Targets ◽

10.2174/1568009618666181102144317 ◽

2019 ◽

Vol 19 (8) ◽

pp. 631-640 ◽

Cited By ~ 1

Author(s):

Omel Baneen Qallandar ◽

Faeza Ebrahimi ◽

Farhadul Islam ◽

Riajul Wahab ◽

Bin Qiao ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Cancer Cells ◽

Alveolar Bone ◽

Cultured Cells ◽

Bone Cells ◽

Bone Invasion ◽

Alveolar Bone Cells

Background: Co-culture of cancer cells with alveolar bone cells could modulate bone invasion and destructions. However, the mechanisms of interaction between oral squamous cell carcinoma (OSCC) and bone cells remain unclear. Objective: The aim of this study is to analyse the direct and indirect effects of OSCC cells in the stimulation of osteolytic activity and bone invasion. Methods: Direct co-culture was achieved by culturing OSCC (TCA8113) with a primary alveolar bone cell line. In the indirect co-culture, the supernatant of TCA8113 cells was collected to culture the alveolar bone cells. To assess the bone invasion properties, in vitro assays were performed. Results: The proliferation of co-cultured cancer cells was significantly (p<0.05) higher in comparison to the monolayer control cells. However, the proliferation rates were not significantly different between direct and indirect co-cultured cells with indirect co-cultured cells proliferated slightly more than the direct co-cultured cells. Invasion and migration capacities of co-cultured OSCC and alveolar bone cells enhanced significantly (p<0.05) when compared to that of control monolayer counterparts. Most importantly, we noted that OSCC cells directly co-cultured with alveolar bone cells stimulated pronounced bone collagen destruction. In addition, stem cells and epithelialmesenchymal transition markers have shown significant changes in their expression in co-cultured cells. Conclusion: In conclusion, the findings of this study highlight the importance of the interaction of alveolar bone cells and OSCC cells in co-culture setting in the pathogenesis of bone invasion. This may help in the development of potential future biotherapies for bone invasion in OSCC.

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In Vitro Compression Model for Orthodontic Tooth Movement Modulates Human Periodontal Ligament Fibroblast Proliferation, Apoptosis and Cell Cycle

Biomolecules ◽

10.3390/biom11070932 ◽

2021 ◽

Vol 11 (7) ◽

pp. 932

Author(s):

Julia Brockhaus ◽

Rogerio B. Craveiro ◽

Irma Azraq ◽

Christian Niederau ◽

Sarah K. Schröder ◽

...

Keyword(s):

Cell Cycle ◽

Cell Death ◽

Periodontal Ligament ◽

Environmental Changes ◽

Tooth Movement ◽

Orthodontic Tooth Movement ◽

Compressive Force ◽

Periodontal Ligament Fibroblasts ◽

Human Periodontal Ligament Fibroblasts

Human Periodontal Ligament Fibroblasts (hPDLF), as part of the periodontal apparatus, modulate inflammation, regeneration and bone remodeling. Interferences are clinically manifested as attachment loss, tooth loosening and root resorption. During orthodontic tooth movement (OTM), remodeling and adaptation of the periodontium is required in order to enable tooth movement. hPDLF involvement in the early phase-OTM compression side was investigated for a 72-h period through a well-studied in vitro model. Changes in the morphology, cell proliferation and cell death were analyzed. Specific markers of the cell cycle were investigated by RT-qPCR and Western blot. The study showed that the morphology of hPDLF changes towards more unstructured, unsorted filaments under mechanical compression. The total cell numbers were significantly reduced with a higher cell death rate over the whole observation period. hPDLF started to recover to pretreatment conditions after 48 h. Furthermore, key molecules involved in the cell cycle were significantly reduced under compressive force at the gene expression and protein levels. These findings revealed important information for a better understanding of the preservation and remodeling processes within the periodontium through Periodontal Ligament Fibroblasts during orthodontic tooth movement. OTM initially decelerates the hPDLF cell cycle and proliferation. After adapting to environmental changes, human Periodontal Ligament Fibroblasts can regain homeostasis of the periodontium, affecting its reorganization.

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