Maturation of rat dendritic cells during intrahepatic translocation evaluated using monoclonal antibodies and electron microscopy

1998 ◽  
Vol 294 (3) ◽  
pp. 503-514 ◽  
Author(s):  
T. Sato ◽  
H. Yamamoto ◽  
C. Sasaki ◽  
K. Wake
Keyword(s):  
Electron Microscopy ◽  
Dendritic Cells ◽  
Monoclonal Antibodies

scholarly journals Cancer Immunotherapy with Immunomodulatory Anti-CD137 and Anti–PD-1 Monoclonal Antibodies Requires BATF3-Dependent Dendritic Cells

2015 ◽  
Vol 6 (1) ◽  
pp. 71-79 ◽  
Author(s):  
Alfonso R. Sánchez-Paulete ◽  
Francisco J. Cueto ◽  
María Martínez-López ◽  
Sara Labiano ◽  
Aizea Morales-Kastresana ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Monoclonal Antibodies ◽  
Cancer Immunotherapy

Definition of individual components within the cytoskeleton of Trypanosoma brucei by a library of monoclonal antibodies

1989 ◽  
Vol 93 (3) ◽  
pp. 491-500 ◽  
Author(s):  
A. Woods ◽  
T. Sherwin ◽  
R. Sasse ◽  
T.H. MacRae ◽  
A.J. Baines ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibodies ◽  
Trypanosoma Brucei ◽  
Immunofluorescence Microscopy ◽  
Complex Mixtures ◽  
Immunogold Electron Microscopy ◽  
Basal Bodies ◽  
Tubulin Isotypes ◽  
Definition Of ◽  
Attachment Zone

The detergent-insoluble T. brucei cytoskeleton consists of several morphologically distinct regions and organelles, many of which are detectable only by electron microscopy. We have produced a set of monoclonal antibodies that define each structural component of this highly ordered cytoskeleton. The monoclonal antibodies were selected by cloning of hybridomas produced from mice injected with complex mixtures of proteins of either the cytoskeleton itself or salt extracts thereof. Four antibodies define particular tubulin isotypes and locate the microtubules of the axoneme and sub-pellicular array; two antibodies recognize the flagellum attachment zone; one recognizes the paraflagellar rod and another the basal bodies. Finally, one antibody defines a detergent-insoluble component of the nucleus. The antigens detected by each monoclonal antibody have been analysed by immunofluorescence microscopy, immunogold electron microscopy and Western blotting.

scholarly journals Application of a Highly Selective Cathepsin S Two-step Activity-Based Probe in Multicolor Bio-Orthogonal Correlative Light-Electron Microscopy

2021 ◽  
Vol 8 ◽  
Author(s):  
Floris J. van Dalen ◽  
Thomas Bakkum ◽  
Tyrza van Leeuwen ◽  
Mirjam Groenewold ◽  
Edgar Deu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Dendritic Cells ◽  
Immune Cell ◽  
Ultrastructural Level ◽  
Cross Reactivity ◽  
Protein Profiling ◽  
Cathepsin S ◽  
Probe Design ◽  
Competitive Activity ◽  
Lysosomal Cysteine Protease

Cathepsin S is a lysosomal cysteine protease highly expressed in immune cells such as dendritic cells, B cells and macrophages. Its functions include extracellular matrix breakdown and cleavage of cell adhesion molecules to facilitate immune cell motility, as well as cleavage of the invariant chain during maturation of major histocompatibility complex II. The identification of these diverse specific functions has brought the challenge of delineating cathepsin S activity with great spatial precision, relative to related enzymes and substrates. Here, the development of a potent and highly selective two-step activity-based probe for cathepsin S and the application in multicolor bio-orthogonal correlative light-electron microscopy is presented. LHVS, which has been reported as a selective inhibitor of cathepsin S with nanomolar potency, formed the basis for our probe design. However, in competitive activity-based protein profiling experiments LHVS showed significant cross-reactivity toward Cat L. Introduction of an azide group in the P2 position expanded the selectivity window for cathepsin S, but rendered the probe undetectable, as demonstrated in bio-orthogonal competitive activity-based protein profiling. Incorporation of an additional azide handle for click chemistry on the solvent-exposed P1 position allowed for selective labeling of cathepsin S. This highlights the influence of click handle positioning on probe efficacy. This probe was utilized in multicolor bio-orthogonal confocal and correlative light-electron microscopy to investigate the localization of cathepsin S activity at an ultrastructural level in bone marrow-derived dendritic cells. The tools developed in this study will aid the characterization of the variety of functions of cathepsin S throughout biology.

scholarly journals Tolerogenic nanoparticles restore the antitumor activity of recombinant immunotoxins by mitigating immunogenicity

2018 ◽  
Vol 115 (4) ◽  
pp. E733-E742 ◽  
Author(s):  
Ronit Mazor ◽  
Emily M. King ◽  
Masanori Onda ◽  
Nicolas Cuburu ◽  
Selamawit Addissie ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Monoclonal Antibodies ◽  
Antitumor Activity ◽  
Regulatory T Cells ◽  
Immune Tolerance ◽  
Checkpoint Inhibitors ◽  
Recombinant Immunotoxins ◽  
Antidrug Antibodies

Protein-based drugs are very active in treating cancer, but their efficacy can be limited by the formation of neutralizing antidrug antibodies (ADAs). Recombinant immunotoxins are proteins that are very effective in patients with leukemia, where immunity is suppressed, but induce ADAs, which compromise their activity, in patients with intact immunity. Here we induced a specific, durable, and transferable immune tolerance to recombinant immunotoxins by combining them with nanoparticles containing rapamycin (SVP-R). SVP-R mitigated the formation of inhibitory ADAs in naïve and sensitized mice, resulting in restoration of antitumor activity. The immune tolerance is mediated by colocalization of the SVP-R and immunotoxin to dendritic cells and macrophages in the spleen and is abrogated by depletion of regulatory T cells. Tolerance induced by SVPs was not blocked by checkpoint inhibitors or costimulatory agonist monoclonal antibodies that by themselves enhance ADA formation.

scholarly journals Aggregation of Human Recombinant Monoclonal Antibodies Influences the Capacity of Dendritic Cells to Stimulate Adaptive T-Cell Responses In Vitro

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e86322 ◽  
Author(s):  
Verena Rombach-Riegraf ◽  
Anette C. Karle ◽  
Babette Wolf ◽  
Laetitia Sordé ◽  
Stephan Koepke ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Monoclonal Antibodies ◽  
T Cell ◽  
T Cell Responses ◽  
Cell Responses
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