A fully integrated microdevice for capturing, amplification, and colorimetric detection of foodborne pathogens

2020 ◽  
Vol 26 (12) ◽  
pp. 3875-3883
Author(s):  
Hoang Chau La ◽  
Nae Yoon Lee
Biosensors ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 443
Author(s):  
Tania Pomili ◽  
Paolo Donati ◽  
Pier Paolo Pompa

In this study, we describe a monolithic and fully integrated paper-based device for the simultaneous detection of three prognostic biomarkers in saliva. The pattern of the proposed multiplexed device is designed with a central sample deposition zone and three identical arms, each containing a pre-treatment and test zone. Its one-step fabrication is realized by CO2 laser cutting, providing remarkable parallelization and rapidity (ca. 5 s/device). The colorimetric detection is based on the sensitive and selective target-induced reshaping of plasmonic multibranched gold nanoparticles, which exhibit a clear spectral shift (and blue-to-pink color change) in case of non-physiological concentrations of the three salivary biomarkers. A rapid and multiplexed naked-eye or smartphone-based readout of the colorimetric response is achieved within 10 min. A prototype kit for POCT testing is also reported, providing robustness and easy handling of the device.


2018 ◽  
Vol 100 ◽  
pp. 96-104 ◽  
Author(s):  
Abkar Sayad ◽  
Fatimah Ibrahim ◽  
Shah Mukim Uddin ◽  
Jongman Cho ◽  
Marc Madou ◽  
...  

Toxins ◽  
2022 ◽  
Vol 14 (1) ◽  
pp. 40
Author(s):  
Valeria Russini ◽  
Carlo Corradini ◽  
Maria Laura De Marchis ◽  
Tatiana Bogdanova ◽  
Sarah Lovari ◽  
...  

Foodborne diseases (FBDs) represent a worldwide public health issue, given their spreadability and the difficulty of tracing the sources of contamination. This report summarises the incidence of foodborne pathogens and toxins found in food, environmental and clinical samples collected in relation to diagnosed or suspected FBD cases and submitted between 2018 and 2020 to the Food Microbiology Unit of the Istituto Zooprofilattico Sperimentale del Lazio e della Toscana (IZSLT). Data collected from 70 FBD investigations were analysed: 24.3% of them started with an FBD diagnosis, whereas a further 41.4% involved clinical diagnoses based on general symptomatology. In total, 5.6% of the 340 food samples analysed were positive for the presence of a bacterial pathogen, its toxins or both. Among the positive samples, more than half involved meat-derived products. Our data reveal the probable impact of the COVID-19 pandemic on the number of FBD investigations conducted. In spite of the serious impact of FBDs on human health and the economy, the investigation of many foodborne outbreaks fails to identify the source of infection. This indicates a need for the competent authorities to continue to develop and implement a more fully integrated health network.


2021 ◽  
pp. 131801
Author(s):  
Wuzhen Qi ◽  
Lingyan Zheng ◽  
Yu Hou ◽  
Hong Duan ◽  
Lei Wang ◽  
...  

Lab on a Chip ◽  
2019 ◽  
Vol 19 (8) ◽  
pp. 1397-1405 ◽  
Author(s):  
Thi Ngoc Diep Trinh ◽  
Nae Yoon Lee

Loop-mediated isothermal amplification combined with fuchsin-based colorimetric detection in a foldable microdevice for rapid and multiplex identification of foodborne pathogens.


2012 ◽  
Vol 84 (6) ◽  
pp. 2900-2907 ◽  
Author(s):  
Jana C. Jokerst ◽  
Jaclyn A. Adkins ◽  
Bledar Bisha ◽  
Mallory M. Mentele ◽  
Lawrence D. Goodridge ◽  
...  

Author(s):  
John A. Reffner ◽  
William T. Wihlborg

The IRμs™ is the first fully integrated system for Fourier transform infrared (FT-IR) microscopy. FT-IR microscopy combines light microscopy for morphological examination with infrared spectroscopy for chemical identification of microscopic samples or domains. Because the IRμs system is a new tool for molecular microanalysis, its optical, mechanical and system design are described to illustrate the state of development of molecular microanalysis. Applications of infrared microspectroscopy are reviewed by Messerschmidt and Harthcock.Infrared spectral analysis of microscopic samples is not a new idea, it dates back to 1949, with the first commercial instrument being offered by Perkin-Elmer Co. Inc. in 1953. These early efforts showed promise but failed the test of practically. It was not until the advances in computer science were applied did infrared microspectroscopy emerge as a useful technique. Microscopes designed as accessories for Fourier transform infrared spectrometers have been commercially available since 1983. These accessory microscopes provide the best means for analytical spectroscopists to analyze microscopic samples, while not interfering with the FT-IR spectrometer’s normal functions.


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