A lateral flow assay for copper(II) utilizing catalytic and stem-loop based signal amplification

2019 ◽  
Vol 186 (2) ◽  
Author(s):  
Yulong Wang ◽  
Limin Wang ◽  
Cunzheng Zhang ◽  
Fengquan Liu
Keyword(s):  
Signal Amplification ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Stem Loop

Enhancing the sensitivity of colorimetric lateral flow assay (CLFA) through signal amplification techniques

2018 ◽  
Vol 6 (44) ◽  
pp. 7102-7111 ◽  
Author(s):  
Haihang Ye ◽  
Xiaohu Xia
Keyword(s):  
Signal Amplification ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Detection Sensitivity

This article highlights recent signal amplification techniques for enhancing the detection sensitivity of colorimetric lateral flow assay.

Duplex-specific nuclease signal amplification-based fluorescent lateral flow assay for the point-of-care detection of microRNAs

The Analyst ◽  
2021 ◽  
Author(s):  
Hongjuan Wei ◽  
Yongjin Peng ◽  
Zikun Bai ◽  
Zhen Rong ◽  
Shengqi Wang
Keyword(s):  
Signal Amplification ◽  
Point Of Care ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Amplification Strategy ◽  
Point Of Care Detection

We demonstrate a fluorescent lateral flow assay combined with duplex specific nuclease signal amplification strategy for high-sensitive point-of-care detection of cancer-related miRNA.

Rapid and sensitive detection of dual lung cancer-associated miRNA biomarkers by a novel SERS-LFA strip coupling with catalytic hairpin assembly signal amplification

2021 ◽  
Vol 9 (10) ◽  
pp. 3661-3671
Author(s):  
Xiaowei Cao ◽  
Yue Sun ◽  
Yu Mao ◽  
Menglin Ran ◽  
Yifan Liu ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Raman Scattering ◽  
Signal Amplification ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Sensitive Detection ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Catalytic Hairpin Assembly

A novel surface-enhanced Raman scattering-lateral flow assay strip in combination with catalytic hairpin assembly signal amplification has been developed for rapid and sensitive detection of miR-196a-5p and miR-31-5p associated with lung cancer.

A signal amplifying fluorescent nanoprobe and lateral flow assay for ultrasensitive detection of cardiac biomarker troponin I

2019 ◽  
Vol 11 (28) ◽  
pp. 3506-3513 ◽  
Author(s):  
Doudou Lou ◽  
Lin Fan ◽  
Yongxin Ji ◽  
Ning Gu ◽  
Yu Zhang
Keyword(s):  
Signal Amplification ◽  
Troponin I ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Detection Sensitivity ◽  
Fluorescence Signal ◽  
Ultrasensitive Detection ◽  
Cardiac Biomarker ◽  
Fluorescent Nanoprobe ◽  
Fluorescence Signal Amplification

Novel functionalized nanoprobes based on a biotin–streptavidin system led to fluorescence signal amplification and the improvement of cTnI detection sensitivity.

Performance comparison of single and mixed-sized gold nanoparticles on straightforward lateral flow assay for albumin detection

2020 ◽  
Author(s):  
Sasima Chotithammakul ◽  
Dakrong Pissuwan
Keyword(s):  
Gold Nanoparticles ◽  
Signal Amplification ◽  
Test Line ◽  
Testing Procedure ◽  
Performance Comparison ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Analytical Performance ◽  
Detection Mechanism ◽  
Multiple Factors

The sensitivity of the lateral flow assay can be influenced by multiple factors, such as the size of gold nanoparticles (GNPs) employed, which affect the sensitivity and reproducibility of the assay testing procedure. Here, we evaluated the analytical performance of single-sized and mixed-sized GNPs using a simple lateral flow assay (LFA) platform. This platform was used as a model assay to diagnose albumin levels and demonstrate the analytical performance of single-sized and mixed-sized GNPs in LFA tests. Two sizes of GNPs@anti-bovine serum albumin (BSA) conjugate proteins were mixed at different ratios. The unique optical properties of the GNPs induced a distinguishing color-shedding effect on the single and mixed-sized GNPs@anti-BSA conjugates interacting with the target analyte BSA spotted on the test line. The use of mixed-sized GNPs@anti-BSA conjugates enhanced signal amplification, and provided superior stability compared with solely employing the large GNPs (50 nm). The proposed platform in this study could provide an efficient BSA detection mechanism that can be utilized as a biomarker for confronting chronic kidney disease.

A Point of Care Lateral Flow Assay for Rapid and Colorimetric Detection of Interleukin 6 and Perspectives in Bedside Diagnostics

2020 ◽  
Author(s):  
Carla Eiras
Keyword(s):  
Interleukin 6 ◽  
Limit Of Detection ◽  
Inflammatory Diseases ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Aggregation Assay ◽  
Before And After ◽  
Bedside Diagnosis

Interleukin-6 (IL-6) is a multifunctional cytokine and high bloodstream levels of which have been associated with severe inflammatory diseases, such as dengue fever, sepsis, various cancers, and visceral leishmaniasis (VL). Rapid tests for the quantification of IL-6 would be of great assistance for the bedside diagnosis and treatment of diseases such as VL. We have developed a lateral flow assay (LFA) for rapid and colorimetric IL-6 detection, consisting of anti-IL-6 antibodies conjugated to gold nanoparticles (AuNPs). The optimal concentration of anti-IL-6 used in the conjugate was determined to be 800.0 μg/mL, based on an aggregation assay using LFA. A linear relationship between IL-6 standard concentration and color intensity was observed after 20 min, with a linear range between 1.25 ng/mL and 9,000 ng/mL. The limit of detection for this method was estimated a t0.38 ng/mL. The concentration of IL-6 in five patients with severe VL was measured using LFA, and the results were consistent with those obtained using the cytometric bead array (CBA) method. A thorough analysis of the LFA membranes’ surface morphology, before and after sample contact, was performed using atomic force microscopy (AFM).The prototype described here is still being tested and improved, but this LFA will undoubtedly be of great help in the clinical quantification of IL-6.

A Point of Care Lateral Flow Assay for Rapid and Colorimetric Detection of Interleukin 6 and Perspectives in Bedside Diagnostics

2020 ◽  
Author(s):  
Carla Eiras
Keyword(s):  
Interleukin 6 ◽  
Limit Of Detection ◽  
Inflammatory Diseases ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Aggregation Assay ◽  
Before And After ◽  
Bedside Diagnosis

Interleukin-6 (IL-6) is a multifunctional cytokine and high bloodstream levels of which have been associated with severe inflammatory diseases, such as dengue fever, sepsis, various cancers, and visceral leishmaniasis (VL). Rapid tests for the quantification of IL-6 would be of great assistance for the bedside diagnosis and treatment of diseases such as VL. We have developed a lateral flow assay (LFA) for rapid and colorimetric IL-6 detection, consisting of anti-IL-6 antibodies conjugated to gold nanoparticles (AuNPs). The optimal concentration of anti-IL-6 used in the conjugate was determined to be 800.0 μg/mL, based on an aggregation assay using LFA. A linear relationship between IL-6 standard concentration and color intensity was observed after 20 min, with a linear range between 1.25 ng/mL and 9,000 ng/mL. The limit of detection for this method was estimated at a t0.38 ng/mL. The concentration of IL-6 in five patients with severe VL was measured using LFA, and the results were consistent with those obtained using the cytometric bead array (CBA) method. A thorough analysis of the LFA membranes’ surface morphology, before and after sample contact, was performed using atomic force microscopy (AFM). The prototype described here is still being tested and improved, but this LFA will undoubtedly be of great help in the clinical quantification of IL-6.

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