Enhancing the sensitivity of colorimetric lateral flow assay (CLFA) through signal amplification techniques

2018 ◽  
Vol 6 (44) ◽  
pp. 7102-7111 ◽  
Author(s):  
Haihang Ye ◽  
Xiaohu Xia
Keyword(s):  
Signal Amplification ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Detection Sensitivity

This article highlights recent signal amplification techniques for enhancing the detection sensitivity of colorimetric lateral flow assay.

A signal amplifying fluorescent nanoprobe and lateral flow assay for ultrasensitive detection of cardiac biomarker troponin I

2019 ◽  
Vol 11 (28) ◽  
pp. 3506-3513 ◽  
Author(s):  
Doudou Lou ◽  
Lin Fan ◽  
Yongxin Ji ◽  
Ning Gu ◽  
Yu Zhang
Keyword(s):  
Signal Amplification ◽  
Troponin I ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Detection Sensitivity ◽  
Fluorescence Signal ◽  
Ultrasensitive Detection ◽  
Cardiac Biomarker ◽  
Fluorescent Nanoprobe ◽  
Fluorescence Signal Amplification

Novel functionalized nanoprobes based on a biotin–streptavidin system led to fluorescence signal amplification and the improvement of cTnI detection sensitivity.

Duplex-specific nuclease signal amplification-based fluorescent lateral flow assay for the point-of-care detection of microRNAs

The Analyst ◽  
2021 ◽  
Author(s):  
Hongjuan Wei ◽  
Yongjin Peng ◽  
Zikun Bai ◽  
Zhen Rong ◽  
Shengqi Wang
Keyword(s):  
Signal Amplification ◽  
Point Of Care ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Amplification Strategy ◽  
Point Of Care Detection

We demonstrate a fluorescent lateral flow assay combined with duplex specific nuclease signal amplification strategy for high-sensitive point-of-care detection of cancer-related miRNA.

Highly sensitive and rapid detection of porcine circovirus 2 by avidin-biotin complex based lateral flow assay coupled to isothermal amplification

2021 ◽  
Author(s):  
Minju Jang ◽  
SeJin Kim ◽  
Junkyu Song ◽  
Sanghyo Kim
Keyword(s):  
Rapid Detection ◽  
Lateral Flow ◽  
Isothermal Amplification ◽  
Lateral Flow Assay ◽  
Detection Sensitivity ◽  
Porcine Circovirus ◽  
Highly Sensitive ◽  
Porcine Circovirus 2 ◽  
Avidin Biotin Complex

In this study, a new platform for the detection of porcine circovirus 2 was developed by avidin-biotin complex based lateral flow assay (LAMP-LFA). Improved detection sensitivity was attained by using...

scholarly journals Microneedle-based skin patch for blood-free rapid diagnostic testing

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Xue Jiang ◽  
Peter B. Lillehoj
Keyword(s):  
Interstitial Fluid ◽  
Point Of Care ◽  
Diagnostic Testing ◽  
Protein Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Detection Sensitivity ◽  
Sample Collection ◽  
Protein Biomarkers ◽  
Skin Patch

Abstract Rapid diagnostic tests are one of the most commonly used tests to detect and screen for infectious diseases in the developing world. While these tests are simple, inexpensive, and readily available, they rely on finger-prick blood sampling, which requires trained medical personnel, poses risks of infection, and can complicate cooperation in young children, asymptomatic individuals, and communities with blood taboos. Here, we report a novel microneedle-based skin patch for the rapid detection of protein biomarkers in dermal interstitial fluid. Sample collection is facilitated by a hydrophilic hollow microneedle array that autonomously extracts and transports interstitial fluid to an antibody-based lateral flow test strip via surface tension for colorimetric antigen detection. We employ a simple gold enhancement treatment to enhance the detection sensitivity of this colloidal gold-based lateral flow assay and elucidate the underlying mechanism of this enhancement mechanism through experimental investigation. For proof-of-concept, this device was used to detect Plasmodium falciparum histidine-rich protein 2, a biomarker for malaria infection, which could be detected at concentrations as low as 8 ng/mL. Each test can be completed in <20 min and requires no equipment. To the best of our knowledge, this work is the first demonstration of a microneedle-based lateral flow assay for rapid protein detection in dermal interstitial fluid. In addition to its simplicity, minimally invasive nature, and low cost, this diagnostic device can be readily adapted to detect other protein biomarkers in interstitial fluid, making it a promising tool for point-of-care testing.

A lateral flow assay for copper(II) utilizing catalytic and stem-loop based signal amplification

2019 ◽  
Vol 186 (2) ◽  
Author(s):  
Yulong Wang ◽  
Limin Wang ◽  
Cunzheng Zhang ◽  
Fengquan Liu
Keyword(s):  
Signal Amplification ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Stem Loop

Rapid and sensitive detection of dual lung cancer-associated miRNA biomarkers by a novel SERS-LFA strip coupling with catalytic hairpin assembly signal amplification

2021 ◽  
Vol 9 (10) ◽  
pp. 3661-3671
Author(s):  
Xiaowei Cao ◽  
Yue Sun ◽  
Yu Mao ◽  
Menglin Ran ◽  
Yifan Liu ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Raman Scattering ◽  
Signal Amplification ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Sensitive Detection ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Catalytic Hairpin Assembly

A novel surface-enhanced Raman scattering-lateral flow assay strip in combination with catalytic hairpin assembly signal amplification has been developed for rapid and sensitive detection of miR-196a-5p and miR-31-5p associated with lung cancer.

scholarly journals Lateral Flow Immunoassay Coupled with Copper Enhancement for Rapid and Sensitive SARS-CoV-2 Nucleocapsid Protein Detection

Biosensors ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 13
Author(s):  
Tao Peng ◽  
Xueshima Jiao ◽  
Zhanwei Liang ◽  
Hongwei Zhao ◽  
Yang Zhao ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Nucleocapsid Protein ◽  
High Efficiency ◽  
Protein Detection ◽  
Antigen Detection ◽  
Lateral Flow ◽  
Copper Deposition ◽  
Detection Sensitivity ◽  
Rapid Screening ◽  
Lateral Flow Immunoassay

The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory coronavirus 2 (SARS-CoV-2) is still raging all over the world. Hence, the rapid and sensitive screening of the suspected population is in high demand. The nucleocapsid protein (NP) of SARS-CoV-2 has been selected as an ideal marker for viral antigen detection. This study describes a lateral flow immunoassay (LFIA) based on colloidal gold nanoparticles for rapid NP antigen detection, in which sensitivity was improved through copper deposition-induced signal amplification. The detection sensitivity of the developed LFIA for NP antigen detection (using certified reference materials) under the optimized parameters was 0.01 μg/mL and was promoted by three orders of magnitude to 10 pg/mL after copper deposition signal amplification. The LFIA coupled with the copper enhancement technique has many merits such as low cost, high efficiency, and high sensitivity. It provides an effective approach to the rapid screening, diagnosis, and monitoring of the suspected population in the COVID-19 outbreak.

Performance comparison of single and mixed-sized gold nanoparticles on straightforward lateral flow assay for albumin detection

2020 ◽  
Author(s):  
Sasima Chotithammakul ◽  
Dakrong Pissuwan
Keyword(s):  
Gold Nanoparticles ◽  
Signal Amplification ◽  
Test Line ◽  
Testing Procedure ◽  
Performance Comparison ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Analytical Performance ◽  
Detection Mechanism ◽  
Multiple Factors

The sensitivity of the lateral flow assay can be influenced by multiple factors, such as the size of gold nanoparticles (GNPs) employed, which affect the sensitivity and reproducibility of the assay testing procedure. Here, we evaluated the analytical performance of single-sized and mixed-sized GNPs using a simple lateral flow assay (LFA) platform. This platform was used as a model assay to diagnose albumin levels and demonstrate the analytical performance of single-sized and mixed-sized GNPs in LFA tests. Two sizes of GNPs@anti-bovine serum albumin (BSA) conjugate proteins were mixed at different ratios. The unique optical properties of the GNPs induced a distinguishing color-shedding effect on the single and mixed-sized GNPs@anti-BSA conjugates interacting with the target analyte BSA spotted on the test line. The use of mixed-sized GNPs@anti-BSA conjugates enhanced signal amplification, and provided superior stability compared with solely employing the large GNPs (50 nm). The proposed platform in this study could provide an efficient BSA detection mechanism that can be utilized as a biomarker for confronting chronic kidney disease.

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