Graphene-based fluorometric determination of agrD gene transcription in methicillin-resistant Staphylococcus aureus using exonuclease III-aided target recycling and DNA walker cascade amplification

2021 ◽  
Vol 188 (8) ◽  
Author(s):  
Yi Ning ◽  
Xiaoqi Wang ◽  
Jue Hu ◽  
Ling Li ◽  
Rong Xiao ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Gene Transcription ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Fluorometric Determination ◽  
Exonuclease Iii ◽  
Methicillin Resistant ◽  
Target Recycling ◽  
Dna Walker ◽  
Cascade Amplification

scholarly journals Phenotypic and Molecular Typing of Nosocomial Methicillin-Resistant Staphylococcus aureus Strains Susceptible to Gentamicin Isolated in France from 1995 to 1997

2000 ◽  
Vol 38 (1) ◽  
pp. 185-190
Author(s):  
Jacques-Olivier Galdbart ◽  
Anne Morvan ◽  
Nevine El Solh
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Typing ◽  
Insertion Sequence ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Phage Typing ◽  
Methicillin Resistant ◽  
Before And After ◽  
Resistant Strains ◽  
Mrsa Strains

ABSTRACT Methicillin-resistant strains susceptible to gentamicin (Gm s MRSA) have emerged since 1993 in several French hospitals. To study whether particular clones have spread in various French cities and whether some clones are related to gentamicin-resistant (Gm r ) MRSA strains, various methods (antibiotyping, phage typing, determination of Sma I macrorestriction patterns before and after hybridization with IS 256 transposase and aacA-aphD probes) were used to compare 62 Gm s MRSA strains isolated from 1995 to 1997 in nine cities and 15 Gm r MRSA strains. Eighteen major Sma I genotypes were identified, of which 11 included only Gm s MRSA strains and 5 included only Gm r MRSA strains. Each of the Gm r MRSA strains contained 6 to 13 Sma I fragments hybridizing with the insertion sequence IS 256 , of which a single band also hybridized with the aacA-aphD gene. No such hybridizing sequences were detected in 60 of the 62 Gm s MRSA strains. Thus, the divergence between Gm r and Gm s MRSA strains is revealed, not only by their distributions in distinct Sma I genotypes but also by the differences in hybridization patterns. Two of the 62 Gm s MRSA strains had the uncommon feature of carrying several Sma I bands hybridizing with IS 256 , suggesting that they are possibly related to the Gm r MRSA strains grouped in the same Sma I genotype. Five of the 11 Sma I genotypes including only Gm s MRSA strains contained strains from diverse cities, isolated during different years and with different antibiograms, suggesting that some clones have spread beyond their cities of origin and persisted.

scholarly journals Determination of Vancomycin and Daptomycin MICs by Different Testing Methods for Methicillin-Resistant Staphylococcus aureus

2011 ◽  
Vol 49 (6) ◽  
pp. 2272-2273 ◽  
Author(s):  
Mark C. Kruzel ◽  
Cole T. Lewis ◽  
Kerry J. Welsh ◽  
Evan M. Lewis ◽  
Nicola E. Dundas ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Testing Methods ◽  
Methicillin Resistant
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