Concentrations of beta-amyloid precursor protein processing products in cerebrospinal fluid of patients with amyotrophic lateral sclerosis and frontotemporal lobar degeneration

2009 ◽  
Vol 116 (9) ◽  
pp. 1169-1178 ◽  
Author(s):  
Petra Steinacker ◽  
Corinna Hendrich ◽  
Anne-Dorte Sperfeld ◽  
Sarah Jesse ◽  
Stefan Lehnert ◽  
...  
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Cerebrospinal Fluid ◽  
Amyloid Precursor Protein ◽  
Frontotemporal Lobar Degeneration ◽  
Beta Amyloid ◽  
Protein Processing ◽  
Precursor Protein ◽  
Amyloid Precursor Protein Processing ◽  
Lateral Sclerosis

scholarly journals Soluble Beta-Amyloid Precursor Protein Is Related to Disease Progression in Amyotrophic Lateral Sclerosis

PLoS ONE ◽  
2011 ◽  
Vol 6 (8) ◽  
pp. e23600 ◽  
Author(s):  
Petra Steinacker ◽  
Lubin Fang ◽  
Jens Kuhle ◽  
Axel Petzold ◽  
Hayrettin Tumani ◽  
...  
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Amyloid Precursor Protein ◽  
Disease Progression ◽  
Beta Amyloid ◽  
Precursor Protein ◽  
Lateral Sclerosis

The chick embryo appears as a natural model for research in beta-amyloid precursor protein processing

Neuroscience ◽  
2005 ◽  
Vol 134 (4) ◽  
pp. 1285-1300 ◽  
Author(s):  
J.A. Carrodeguas ◽  
A. Rodolosse ◽  
M.V. Garza ◽  
A. Sanz-Clemente ◽  
R. Pérez-Pé ◽  
...  
Keyword(s):  
Chick Embryo ◽  
Amyloid Precursor Protein ◽  
Beta Amyloid ◽  
Protein Processing ◽  
Precursor Protein ◽  
Amyloid Precursor Protein Processing

Cell line construction and maintenance for Lyso-IP and Endo-IP analysis of amyloid precursor protein processing v1

2021 ◽  
Author(s):  
Hankum Park ◽  
Frances V Hundley ◽  
Harper JW
Keyword(s):  
Amyloid Precursor Protein ◽  
Cell Line ◽  
Quantitative Proteomics ◽  
Protein Processing ◽  
Precursor Protein ◽  
Amyloid Precursor Protein Processing ◽  
App Processing ◽  
293 Cells ◽  
Line Construction ◽  
App Gene

Lyso-IP is a method that allows for the isolation of lysosomes for proteomics and metabolomics (dx.doi.org/10.17504/protocols.io.bybjpskn; dx.doi.org/10.17504/protocols.io.bx9hpr36). We have developed an analogous approach for purification of early/sorting endosomes (Endo-IP). In addition, we have found that endolysosomal purification via Lyso-IP and Endo-IP can be coupled with a quantitative proteomics workflow to obtain snapshots of Amyloid Precursor Protein (APP) processing to its Aβ products (Park et al. in submission). Here, we describe methods for cell line construction and maintenance of 293 cells with TMEM192-3xHA and 3xFLAG-EEA1, which are used for lysosome and endosome purification, respectively, with the addition of patient mutations to APP promotes processing. Cells with endogenously tagged TMEM192 and stably expressing FLAG-EEA1 are referred to as 293EL cells, for Endo-IP and Lyso-IP. These cells were also prepared in a form that has a deletion of the APP gene (293EL;APP-/-) and the same cells reconstituted with a lentivirus stably expressing APPSw;T700N to allow functional analysis of APP processing.

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