A major-capsid-protein-based multiplex PCR assay for rapid identification of selected virulent bacteriophage types

Acinetobacter baumannii, Acinetobacter nosocomialis and Acinetobacter pittii are clinically relevant members of the Acinetobacter calcoaceticus–A. baumannii (Acb) complex and important nosocomial pathogens. These three species are genetically closely related and phenotypically similar; however, they differ in their epidemiology, antibiotic resistance and pathogenicity. In this study, we investigated the use of a multiplex PCR-based assay designed to detect internal fragments of the 16S–23S rRNA intergenic region and the gyrB and recA genes. The assay was capable of differentiating A. baumannii, A. nosocomialis and A. pittii in a reliable manner. In 23 different reference strains and 89 clinical isolates of Acinetobacter species, the assay accurately identified clinically relevant Acb complex species except those ‘between 1 and 3’ or ‘close to 13TU’. None of the non-Acb complex species was misidentified. In an analysis of 1034 positive blood cultures, the assay had a sensitivity of 92.4 % and specificity of 98.2 % for Acb complex identification. Our results show that a single multiplex PCR assay can reliably differentiate clinically relevant Acb complex species. Thus, this method may be used to better understand the clinical differences between infections caused by these species.

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Accurate and Rapid Identification of Longan Arillus and Litchi Semen by a Multiplex PCR Assay

Plants ◽

10.3390/plants9080948 ◽

2020 ◽

Vol 9 (8) ◽

pp. 948

Author(s):

Wook Jin Kim ◽

Sungyu Yang ◽

Goya Choi ◽

Inkyu Park ◽

Pureum Noh ◽

...

Keyword(s):

Multiplex Pcr ◽

Dna Markers ◽

Herbal Medicines ◽

High Specificity ◽

Single Species ◽

Rapid Identification ◽

Genetic Identification ◽

Pcr Assay ◽

Multiplex Pcr Assay ◽

Specificity And Sensitivity

Dimocarpus longan, Litchi chinensis, and Nephelium lappaceum are commercially valuable subtropical and tropical fruits of the Sapindaceae family. Arillus and seeds of the three species have very similar morphologies; however, the arillus of D. longan is used as the herbal medicine Longan Arillus and seeds of L. chinensis are used as Litchi Semen in Korean and Chinese pharmacopoeias. The adulteration of herbal medicines with inauthentic species, including the use of Aril and seed fractions acquired from a single species for two herbal medicines (e.g., Longan Arillus and Litchi Semen), is often driven by economic motives. DNA markers are a tool for the detection of adulterants in commercial products. To establish rapid and reliable assays for the genetic identification of authentic Longan Arillus and Litchi Semen, we developed DNA markers with high specificity and sensitivity based on internal transcribed spacer (ITS) sequences. The newly developed DNA markers and multiplex PCR assay may contribute to efforts to protect against adulteration, quality control, and the standardization of herbal medicines.

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Multiplex PCR assay for rapid identification of three endangered snake species of India

Conservation Genetics ◽

10.1007/s10592-009-9835-y ◽

2009 ◽

Vol 10 (6) ◽

pp. 1861-1864 ◽

Cited By ~ 17

Author(s):

Bhawna Dubey ◽

P. R. Meganathan ◽

Ikramul Haque

Keyword(s):

Multiplex Pcr ◽

Rapid Identification ◽

Pcr Assay ◽

Snake Species ◽

Multiplex Pcr Assay

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Development of a Multiplex-PCR assay for the rapid identification of Geobacillus stearothermophilus and Anoxybacillus flavithermus

Food Microbiology ◽

10.1016/j.fm.2014.05.002 ◽

2014 ◽

Vol 43 ◽

pp. 41-49 ◽

Cited By ~ 4

Author(s):

Carmela Pennacchia ◽

Pieter Breeuwer ◽

Rolf Meyer

Keyword(s):

Multiplex Pcr ◽

Rapid Identification ◽

Geobacillus Stearothermophilus ◽

Pcr Assay ◽

Multiplex Pcr Assay ◽

Anoxybacillus Flavithermus

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Rapid Detection of Bloodstream Pathogens in Oncologic Patients with a FilmArray Multiplex PCR Assay: a Comparison with Culture Methods

Polish Journal of Microbiology ◽

10.5604/01.3001.0011.6149 ◽

2018 ◽

Vol 67 (1) ◽

pp. 103-107 ◽

Cited By ~ 1

Author(s):

Maria Szymankiewicz ◽

Beata Nakonowska

Keyword(s):

Blood Culture ◽

Multiplex Pcr ◽

Accurate Method ◽

Rapid Identification ◽

Blood Cultures ◽

Pcr Assay ◽

Culture Methods ◽

Multiplex Pcr Assay ◽

Oncologic Patients ◽

Culture Identification

The results of the FilmArray® Blood Culture Identification Panel (BCID) (BioFire Diagnostics) and the culture with susceptibility testing of 70 positive blood cultures from oncologic patients were compared. The multiplex PCR assay (BCID) identified 81 of the 83 isolates (97.6%), covered by the panel. The panel produced results in significantly shorter time than standard identification methods, when counted from receiving positive blood cultures bottles to the final results. It is an accurate method for the rapid identification of pathogens and resistance genes from blood culture in oncologic patients.

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