scholarly journals Gene expression pattern differences in primary human pulmonary epithelial cells infected with MERS-CoV or SARS-CoV-2

2020 ◽  
Vol 165 (10) ◽  
pp. 2205-2211 ◽  
Author(s):  
Yunyueng Jang ◽  
Sang Heui Seo
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Expression Pattern ◽  
Gene Expression Pattern ◽  
Pulmonary Epithelial Cells

Concurrence between the gene expression pattern of Actinobacillus actinomycetemcomitans in localized aggressive periodontitis and in human epithelial cells

2005 ◽  
Vol 54 (5) ◽  
pp. 497-504 ◽  
Author(s):  
Joseph Richardson ◽  
Justin Corey Craighead ◽  
Sam Linsen Cao ◽  
Martin Handfield
Keyword(s):  
Gene Expression ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Expression Pattern ◽  
Gene Expression Pattern ◽  
Aggressive Periodontitis ◽  
Actinobacillus Actinomycetemcomitans ◽  
Bacterial Gene ◽  
Bacterial Gene Expression

Actinobacillus actinomycetemcomitans is a facultatively intracellular pathogen and the aetiological agent of localized aggressive periodontitis. Screening of the genome of A. actinomycetemcomitans for in vivo-induced antigen determinants previously demonstrated that the proteome of this organism differs in laboratory culture compared with conditions found during active infection. The aim of the present study was to determine whether the bacterial gene expression pattern inferred with in vivo-induced antigen technology (IVIAT) in human infections was consistent with the gene expression pattern occurring upon epithelial cell association. To this end, a real-time PCR method was developed and used to quantify absolute and relative bacterial gene expression of A. actinomycetemcomitans grown extra- and intracellularly in two human epithelial cell lines (HeLa and IHGK). The amount of template used in the assay was normalized using the total count of viable bacteria (c.f.u.) as a reference point and performed in duplicate in at least two independent experiments. Controls for this experiment included 16S rRNA and gapdh. Transcription of all eight ORFs tested increased significantly (P < 0.05) in HeLa and IHGK cells compared with bacteria grown extracellularly. The concurrence of gene expression patterns found in the two models suggests that these epithelial cells are valid in vitro models of infection for the genes tested. IVIAT is an experimental platform that can be used as a validation tool to assess the reliability of animal and other models of infection and is applicable to most pathogens.

The Human Embryo-Endometrial Dialogue: Impact of a Single Blastocyst in the Gene Expression Pattern of Endometrial Epithelial Cells

2005 ◽  
Vol 84 ◽  
pp. S60-S61 ◽  
Author(s):  
J.A. Horcajadas ◽  
R. Catalano ◽  
B. Gadea ◽  
A. Sharkey ◽  
A. Pellicer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Expression Pattern ◽  
Human Embryo ◽  
Gene Expression Pattern ◽  
Endometrial Epithelial Cells

Gene Expression Pattern Induced by Leptin in Human Endometrial Epithelial Cells

2005 ◽  
Vol 84 ◽  
pp. S435
Author(s):  
A. Cervero ◽  
J.A. Horcajadas ◽  
R. Catalano ◽  
A. Sharkey ◽  
A. Pellicer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Expression Pattern ◽  
Gene Expression Pattern ◽  
Endometrial Epithelial Cells

Changes in the gene expression pattern of cytokeratins in human respiratory epithelial cells during culture

2004 ◽  
Vol 262 (5) ◽  
pp. 390-396 ◽  
Author(s):  
Michaela Endres ◽  
Iris Leinhase ◽  
Christian Kaps ◽  
Marek Wentges ◽  
Manja Unger ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Expression Pattern ◽  
Gene Expression Pattern ◽  
Respiratory Epithelial Cells ◽  
Human Respiratory Epithelial Cells ◽  
Respiratory Epithelial

Interactions between cadmium and zinc on gene expression pattern of differentiation markers in MC3T3-E1 cell line

Xenobiotica ◽  
2021 ◽  
pp. 1-28
Author(s):  
Sana Boughammoura ◽  
Mylène Zarka ◽  
Imed Messaoudi ◽  
Martine Cohen Solal
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Expression Pattern ◽  
Gene Expression Pattern ◽  
Differentiation Markers
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