scholarly journals Genetic analysis of human parainfluenza virus type 4 associated with severe acute respiratory infection in children in Luohe City, Henan Province, China, during 2017–2018

Author(s):  
Shanshan Zhou ◽  
Naiying Mao ◽  
Yan Zhang ◽  
Aili Cui ◽  
Zhen Zhu ◽  
...  

AbstractDuring 2017–2018, nasopharyngeal aspirates (NPAs) from 627 hospitalized patients with severe acute respiratory infection at Luohe Center Hospital were tested by RT-PCR for human parainfluenza virus 4 (HPIV-4). Fourteen (2.2%) of the 627 samples were positive for HPIV-4. The complete HN gene was amplified from nine positive samples and sequenced. Sequence comparisons showed that the HPIV-4 strains circulating in the city of Luohe are closely related to HPIV-4A strains. Our study indicated that there were multiple lineages of HPIV-4 circulating in Henan Province in China during the study period. This will improve our understanding of the epidemiological and clinical characteristics of HPIV-4.

2021 ◽  
Author(s):  
Shanshan Zhou ◽  
Naiying Mao ◽  
Yan Zhang ◽  
Aili Cui ◽  
Zhen Zhu ◽  
...  

Abstract During 2017–2018, Nasopharyngeal aspirates (NPAs) from 627 hospitalized patients with SARI at Luohe Center Hospital were tested by RT-PCR for human parainfluenza virus 4(HPIV-4). 14 (2.2%) of 627 samples were positive for HPIV-4. The complete nucleotide sequence of the HN gene from 9 positive samples was amplified and sequenced successfully. Genetic analysis showed that the HPIV-4 strains circulating in Luohe city are more closely related to HPIV-4A. Our study indicated that there were multiple lineages of HPIV-4 circulating in Henan Province in China during the study period, which will improve our understanding of the epidemiological and clinical characteristics of HPIV-4.


2017 ◽  
Vol 5 (33) ◽  
Author(s):  
J. L. Kennedy ◽  
J. C. Kincaid ◽  
K. C. Schwalm ◽  
A. N. Stoner ◽  
T. J. Abramo ◽  
...  

ABSTRACT Using target capture of viral nucleic acid and next-generation sequencing, we generated the genome sequences of three novel human parainfluenza virus 2 isolates. Isolates ACRI_0185 (GenBank accession number MF077311), ACRI_0230 (MF077312), and ACRI_0248 (MF077313) were collected in October 2016, February 2017, and March 2017, respectively, from pediatric patients with acute respiratory infection in Arkansas.


2016 ◽  
Vol 4 (5) ◽  
Author(s):  
J. L. Denson ◽  
J. L. Kennedy ◽  
W. N. Dehority ◽  
M. M. Eickman ◽  
K. S. Schwalm ◽  
...  

Using target capture of viral nucleic acid and next-generation sequencing, we generated the complete genomes of two novel human parainfluenza virus 1 isolates. Isolates AR001 (accession no. KX570602) and NM001 (accession no. KX639498) were collected 3 months apart from pediatric patients with acute respiratory infection from Arkansas and New Mexico, respectively.


2020 ◽  
Vol 15 (1) ◽  
pp. 34-44
Author(s):  
Abu Tholib Aman ◽  
Tri Wibawa ◽  
Herman Kosasih ◽  
Rizka Humardewayanti Asdie ◽  
Ida Safitri ◽  
...  

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