Plasma free amino acid kinetics in rainbow trout (Oncorhynchus mykiss) using a bolus injection of 15N-labeled amino acids

Amino Acids ◽  
2010 ◽  
Vol 40 (2) ◽  
pp. 689-696 ◽  
Author(s):  
Jacob William Robinson ◽  
Dan Yanke ◽  
Jeff Mirza ◽  
James Stuart Ballantyne
Keyword(s):  
Amino Acids ◽  
Rainbow Trout ◽  
Amino Acid ◽  
Oncorhynchus Mykiss ◽  
Free Amino Acid ◽  
Free Amino ◽  
Bolus Injection ◽  
Rainbow Trout Oncorhynchus Mykiss

Effect of feeding on the tissue free amino acid concentrations in rainbow trout (Oncorhynchus mykiss Walbaum)

1995 ◽  
Vol 14 (2) ◽  
pp. 153-164 ◽  
Author(s):  
C. G. Carter ◽  
Z-Y. He ◽  
D. F. Houlihan ◽  
I. D. McCarthy ◽  
I. Davidson
Keyword(s):  
Rainbow Trout ◽  
Amino Acid ◽  
Oncorhynchus Mykiss ◽  
Free Amino Acid ◽  
Free Amino ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Amino Acid Concentrations ◽  
Effect Of Feeding

DETERMINATION OF PROTEIN SYNTHESIS IN RAINBOW TROUT, ONCORHYNCHUS MYKISS, USING A STABLE ISOTOPE

1994 ◽  
Vol 189 (1) ◽  
pp. 279-284
Author(s):  
C Carter ◽  
S Owen ◽  
Z He ◽  
P Watt ◽  
C Scrimgeour ◽  
...  
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Rainbow Trout ◽  
Amino Acid ◽  
Oncorhynchus Mykiss ◽  
Considerable Proportion ◽  
Published Data ◽  
Short Term ◽  
Terrestrial Mammals ◽  
Rainbow Trout Oncorhynchus Mykiss

It has been suggested (Houlihan, 1991) that the consumption of 1 g of protein in a variety of species of fish stimulates the synthesis of, approximately, an equal amount of protein. Although synthesis of protein may account for as much as 40 % of the whole-animal oxygen consumption (Lyndon et al. 1992), only about 30 % of the synthesized proteins are retained as growth (Houlihan et al. 1988; Carter et al. 1993a,b). Thus, one focus of attention is the potential advantage gained by fish in allocating a considerable proportion of assimilated energy to protein turnover in contrast to relatively low-cost, low-turnover protein growth (Houlihan et al. 1993). Rates of protein synthesis in several species of fish have been measured using radioactively labelled amino acids, frequently given as a flooding dose (reviewed by Fauconneau, 1985; Houlihan, 1991). These measurements cannot be made for longer than a few hours because of the decline in specific radioactivity in the amino acid free pool. However, as protein synthesis rates vary during the course of a day as a result of the post-prandial stimulation, and since radiolabelled amino acid methodology is invasive, short-term and terminal, it has been difficult to be certain of the relationship between protein growth measured in the long term and protein synthesis rates measured in the short term. This paper addresses these problems by developing a method using 15N in orally administered protein to measure protein synthesis rates in fish over relatively long periods, the aim being to use procedures that are as non-invasive and repeatable as possible. The use of stable isotopes to measure protein metabolism is well established in terrestrial mammals (see Rennie et al. 1991; Wolfe, 1992), but to our knowledge the only published data for aquatic ectotherms are on the blue mussel (Mytilus edulis L.) (Hawkins, 1985). In the present study, rates of protein synthesis of individual rainbow trout [Oncorhynchus mykiss (Walbaum)] were calculated from the enrichment of excreted ammonia with 15N over the 48 h following the feeding of a single meal (dose) containing protein uniformly labelled with 15N by use of an end-point stochastic model (Waterlow et al. 1978; Wolfe, 1992). Application of this type of modelling would appear to be ideal for measuring ammonotelic fish nitrogen metabolism since, unlike the situation in mammals, the catabolic flux of amino acids through urea is very small. Further, ammonia is excreted directly into the surrounding water via the gills and is not stored for any length of time, in contrast to the situation in mammals, so the rate of tracer appearance is easily measurable.

scholarly journals Effect of Pantothenic Acid Deficiency on Plasma Free Amino Acid Profile in Rainbow Trout Oncorhynchus mykiss

1999 ◽  
Vol 65 (5) ◽  
pp. 794-795 ◽  
Author(s):  
Toshiro Masumoto ◽  
Takeo Miki ◽  
Yoshiaki Itoh ◽  
Hidetsuyo Hosokawa ◽  
Sadao Shimeno
Keyword(s):  
Rainbow Trout ◽  
Amino Acid ◽  
Oncorhynchus Mykiss ◽  
Free Amino Acid ◽  
Free Amino ◽  
Pantothenic Acid ◽  
Amino Acid Profile ◽  
Acid Deficiency ◽  
Pantothenic Acid Deficiency
Sign in / Sign up

Export Citation Format

Share Document