Comparison between thin-layer chromatography and overpressured layer chromatography fingerprints of commercial essential oils and accelerated solvent extraction plant extracts

Abstract Thin-layer chromatography on silica gel may be used for the determination of chlorinated pesticide residues in some plant extracts without prior cleanup, thus eliminating the possible loss of pesticides during the cleanup procedure. Rapid screening of a large number of samples is thus possible with consequent saving of time and labor. When necessary, thin-layer chromatography may be used as a prior cleanup for gas chromatography.

Download Full-text

Thin-Layer Chromatography-Densitometry as a Powerful Method for the Standardization of Medicinal Plant Extracts

Recent Advances in Thin-Layer Chromatography ◽

10.1007/978-1-4899-2221-2_20 ◽

1988 ◽

pp. 187-200

Author(s):

M. Vanhaelen ◽

R. Vanhaelen-Fastré

Keyword(s):

Medicinal Plant ◽

Thin Layer ◽

Thin Layer Chromatography ◽

Plant Extracts ◽

Powerful Method ◽

Medicinal Plant Extracts ◽

Layer Chromatography

Download Full-text

Simplified Method for Microscale Production and Quantification of Aflatoxin in Broth1

Journal of Food Protection ◽

10.4315/0362-028x-47.7.526 ◽

1984 ◽

Vol 47 (7) ◽

pp. 526-529 ◽

Cited By ~ 8

Author(s):

WEI-YUN J. TSAI ◽

JIMMY D. LAMBERT ◽

LLOYD B. BULLERMAN

Keyword(s):

Solvent Extraction ◽

Thin Layer ◽

Thin Layer Chromatography ◽

Frozen Storage ◽

Aflatoxin Production ◽

Production Studies ◽

Simplified Method ◽

Tlc Plates ◽

Layer Chromatography ◽

Yeast Extract Sucrose

A simplified method for aflatoxin production studies is described. The mold was cultured in 4-dram (15 ml) vials containing 5 ml of yeast extract sucrose broth, and aflatoxin levels were determined by direct spotting of the broth on thin layer chromatography (TLC) plates and quantitating by spectrodensitometry. Equivalent levels of aflatoxins were produced in vials as compared to flasks. When compared to conventional TLC after solvent extraction, direct spotting was rapid, economical and statistically equivalent. Heating broth cultures (121°C, 15 s) before TLC improved the release of aflatoxin from mycelial mats. Aflatoxins were unstable in YES broth during 3 months of frozen storage.

Download Full-text

Sodium Methylate Treatment of Cleaned Up Plant Extracts in Confirmation of Some Pesticide Residues By Gas-Liquid and Thin Layer Chromatography

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/51.5.1095 ◽

1968 ◽

Vol 51 (5) ◽

pp. 1095-1101

Author(s):

C E Mendoza ◽

P J Wales ◽

H A Mcleod ◽

W P Mckinley

Keyword(s):

Thin Layer ◽

Thin Layer Chromatography ◽

Plant Extracts ◽

Pesticide Residues ◽

Sodium Methylate ◽

Heptachlor Epoxide ◽

Rapid Procedure ◽

Qualitative Confirmation ◽

Layer Chromatography

Abstract A rapid procedure that involves GLC-EC and TLC analyses of plant extracts 5 min after treatment with sodium methylate is described for the qualitative confirmation of some pesticide residues. Plant extracts are cleaned up by elution through a carboncellulose column before treatment. The compounds studied were aldrin, captan, carbophenothion, p,p'-DDD, de-hydrochlorinated p,p-DDD, p,p'-DDE, o,p -DDT, p,p -DDT, Diazinon, dieldrin, disulfoton, heptachlor, heptachlor epoxide, malathion, methoxychlor, de-hydrochlorinated methoxychlor, mevinphos, and parathion. The pesticides added to beet, lettuce, pineapple, potato, and wheat eluates were reliably confirmed by GLC-EC or TLC after sodium methylate treatment. The procedure could be used to confirm only some of the pesticides added to extracts of apples, carrots, cauliflower, grapes, and peas because of components in these samples that gave interfering peaks

Download Full-text