Synthetic analogues of the histidine–chlorophyll complex: a NMR study to mimic structural features of the photosynthetic reaction center and the light-harvesting complex

2003 ◽  
Vol 9 (1) ◽  
pp. 109-117 ◽  
Author(s):  
Adriaan J. van Gammeren ◽  
Frans B. Hulsbergen ◽  
Cornelis Erkelens ◽  
Huub J. M. de Groot
Keyword(s):  
Reaction Center ◽  
Light Harvesting ◽  
Photosynthetic Reaction Center ◽  
Structural Features ◽  
Synthetic Analogues ◽  
Light Harvesting Complex ◽  
Nmr Study

scholarly journals The PuhB Protein of Rhodobacter capsulatus Functions in Photosynthetic Reaction Center Assembly with a Secondary Effect on Light-Harvesting Complex 1

2005 ◽  
Vol 187 (4) ◽  
pp. 1334-1343 ◽  
Author(s):  
Muktak Aklujkar ◽  
Roger C. Prince ◽  
J. Thomas Beatty
Keyword(s):  
Reaction Center ◽  
Rhodobacter Capsulatus ◽  
Light Harvesting ◽  
Expression System ◽  
Photosynthetic Apparatus ◽  
Photosynthetic Reaction Center ◽  
Growth Conditions ◽  
Physiological Adaptation ◽  
Light Harvesting Complex ◽  
The Core

ABSTRACT The core of the photosynthetic apparatus of purple photosynthetic bacteria such as Rhodobacter capsulatus consists of a reaction center (RC) intimately associated with light-harvesting complex 1 (LH1) and the PufX polypeptide. The abundance of the RC and LH1 components was previously shown to depend on the product of the puhB gene (formerly known as orf214). We report here that disruption of puhB diminishes RC assembly, with an indirect effect on LH1 assembly, and reduces the amount of PufX. Under semiaerobic growth conditions, the core complex was present at a reduced level in puhB mutants. After transfer of semiaerobically grown cultures to photosynthetic (anaerobic illuminated) conditions, the RC/LH1 complex became only slightly more abundant, and the amount of PufX increased as cells began photosynthetic growth. We discovered that the photosynthetic growth of puhB disruption strains of R. capsulatus starts after a long lag period, which is due to physiological adaptation rather than secondary mutations. Using a hybrid protein expression system, we determined that the three predicted transmembrane segments of PuhB are capable of spanning a cell membrane and that the second transmembrane segment could mediate self-association of PuhB. We discuss the possible function of PuhB as a dimeric RC assembly factor.

Enhancing the Light Harvesting Capability of a Photosynthetic Reaction Center by a Tailored Molecular Fluorophore

2012 ◽  
Vol 51 (44) ◽  
pp. 11019-11023 ◽  
Author(s):  
Francesco Milano ◽  
Rocco Roberto Tangorra ◽  
Omar Hassan Omar ◽  
Roberta Ragni ◽  
Alessandra Operamolla ◽  
...  
Keyword(s):  
Reaction Center ◽  
Light Harvesting ◽  
Photosynthetic Reaction Center
Sign in / Sign up

Export Citation Format

Share Document