scholarly journals Biofunctionalization of porcine-derived collagen matrices with platelet rich fibrin: influence on angiogenesis in vitro and in vivo

2020 ◽  
Vol 24 (10) ◽  
pp. 3425-3436 ◽  
Author(s):  
Sebastian Blatt ◽  
Valentin Burkhardt ◽  
Peer W. Kämmerer ◽  
Andreas M. Pabst ◽  
Keyvan Sagheb ◽  
...  
Keyword(s):  
Growth Factor ◽  
Immunohistochemical Staining ◽  
Collagen Matrices ◽  
Platelet Rich Fibrin ◽  
Angiogenic Potential ◽  
Branching Points ◽  
Growth Factor Release ◽  
Factor Release

Abstract Objectives Porcine-derived collagen matrices (CM) can be used for oral tissue regeneration, but sufficient revascularization is crucial. The aim of this study was to analyze the influence of platelet-rich fibrin (PRF) on angiogenesis of different CM in vitro and in vivo. Materials and methods Three different CM (mucoderm, jason, collprotect) were combined with PRF in a plotting process. Growth factor release (VEGF, TGF-β) was measured in vitro via ELISA quantification after 1,4 and 7 days in comparison to PRF alone. In ovo yolk sac (YSM) and chorion allantois membrane (CAM) model, angiogenic potential were analyzed in vivo with light- and intravital fluorescence microscopy after 24 h, then verified with immunohistochemical staining for CD105 and αSMA. Results Highest growth factor release was seen after 24 h for all three activated membranes in comparison to the native CM (VEGF 24 h: each p < 0.05; TGF-β: each p < 0.001) and the PRF (no significant difference). All activated membranes revealed a significantly increased angiogenic potential in vivo after 24 h (vessels per mm2: each p < 0.05; branching points per mm2: each p < 0.01; vessel density: each p < 0.05) and with immunohistochemical staining for CD105 (each p < 0.01) and αSMA (each p < 0.05). Conclusions PRF improved the angiogenesis of CM in vitro and in vivo. Clinical relevance Bio-functionalization of CM with PRF could easily implemented in the clinical pathway and may lead to advanced soft tissue healing.

scholarly journals Heparin‐regulated growth factor release in vitro and angiogenesis in vivo from hyaluronan hydrogels

2007 ◽  
Vol 21 (5) ◽  
Author(s):  
Daniel B. Pike ◽  
Shenshen Cai ◽  
Kyle R. Pomraning ◽  
Matthew A. Firpo ◽  
Xiao Zheng Shu ◽  
...  
Keyword(s):  
Growth Factor ◽  
Growth Factor Release ◽  
Release In Vitro ◽  
Factor Release

scholarly journals Does Platelet-Rich Fibrin Enhance the Early Angiogenetic Potential of Different Bone Substitute Materials? An In Vitro and In Vivo Analysis

Biomedicines ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 61
Author(s):  
Sebastian Blatt ◽  
Daniel G.E. Thiem ◽  
Andreas Pabst ◽  
Bilal Al-Nawas ◽  
Peer W. Kämmerer
Keyword(s):  
Growth Factor ◽  
Bone Substitute ◽  
Tissue Growth ◽  
Platelet Rich Fibrin ◽  
Vessel Formation ◽  
Branching Points ◽  
Bone Substitute Materials ◽  
Substitute Materials

The impaired angiogenic potential of bone substitute materials (BSMs) may limit regenerative processes. Therefore, changes in the angiogenetic properties of different BSMs in combination with platelet-rich fibrin (PRF) in comparison to PRF alone, as well as to native BSMs, were analyzed in vitro and in vivo to evaluate possible clinical application. In vitro, four BSMs of different origins (allogeneic, alloplastic, and xenogeneic) were biofunctionalized with PRF and compared to PRF in terms of platelet interaction and growth factor release (vascular endothelial growth factor (VEGF), tissue growth factor ß (TGFß) and platelet-derived growth factor (PDGF)) after 15 min. To visualize initial cell–cell interactions, SEM was performed. In vivo, all BSMs (±PRF) were analyzed after 24 h for new-formed vessels using a chorioallantoic membrane (CAM) assay. Especially for alloplastic BSMs, the addition of PRF led to a significant consumption of platelets (p = 0.05). PDGF expression significantly decreased in comparison to PRF alone (all BSMs: p < 0.013). SEM showed the close spatial relation of each BSM and PRF. In vivo, PRF had a significant positive pro-angiogenic influence in combination with alloplastic (p = 0.007) and xenogeneic materials (p = 0.015) in comparison to the native BSMs. For bio-activated xenogeneic BSMs, the branching points were also significantly increased (p = 0.005). Finally, vessel formation was increased for BSMs and PRF in comparison to the native control (allogeneic: p = 0.046; alloplastic: p = 0.046; and xenogeneic: p = 0.050). An early enhancement of angiogenetic properties was demonstrated when combining BSMs with PRF in vitro and led to upregulated vessel formation in vivo. Thus, the use of BSMs in combination with PRF may trigger bony regeneration in clinical approaches.

Bone-derived growth factor release from poly(α-hydroxy acid) implants in vitro

Biomaterials ◽  
1993 ◽  
Vol 14 (3) ◽  
pp. 177-183 ◽  
Author(s):  
M.C. Meikle ◽  
W.-Y. Mak ◽  
S. Papaioannou ◽  
E.H. Davies ◽  
N. Mordan ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hydroxy Acid ◽  
Growth Factor Release ◽  
Factor Release

428 GROWTH FACTOR RELEASE FROM A CHEMICALLY MODIFIED ELASTOMERIC POLY (DIOL CITRATE) SCAFFOLD PROMOTES ANGIOGENESIS IN VIVO

2011 ◽  
Vol 185 (4S) ◽  
Author(s):  
David Kollhoff ◽  
Matthew Bury ◽  
Natalie Fuller ◽  
Partha Hota ◽  
Natalie Tapaskar ◽  
...  
Keyword(s):  
Growth Factor ◽  
Chemically Modified ◽  
Growth Factor Release ◽  
Factor Release

scholarly journals 4D printing of stretchable nanocookie@conduit material hosting biocues and magnetoelectric stimulation for neurite sprouting

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Jen-Hung Fang ◽  
Hao-Hsiang Hsu ◽  
Ru-Siou Hsu ◽  
Chih-Kang Peng ◽  
Yu-Jen Lu ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Fate ◽  
Electromagnetic Stimulation ◽  
Frequency Magnetic Field ◽  
Growth Factor Release ◽  
Differentiation And Proliferation ◽  
Dimensional Printing ◽  
Proliferation In Vitro

Abstract A high-frequency magnetic field (MF) generates an electric current by charging conductors that enable the induction of various biological processes, including changes in cell fate and programming. In this study, we show that electromagnetized carbon porous nanocookies (NCs) under MF treatment facilitate magnetoelectric conversion for growth factor release and cell stimulation to induce neuron cell differentiation and proliferation in vitro and in vivo. Integrating four-dimensional printing technology, the NCs are exposed on the surface, which enhances the cell adhesion and allows direct manipulation of electromagnetic stimulation of the cells. Remarkably, large amounts of growth factor encapsulated in NC@conduit resulted in excellent permeability and on-demand release, improving the in vivo layers of myelin sheaths and directing the axon orientation at 1 month postimplantation. This study offers proof of principle for MF-guided in vivo neuron regeneration as a potentially viable tissue regeneration approach for neuronal diseases.

Platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in human cell cultures: Growth factor release and contradictory results

2010 ◽  
Vol 110 (4) ◽  
pp. 418-421 ◽  
Author(s):  
David M. Dohan Ehrenfest ◽  
Marco Del Corso ◽  
Francesco Inchingolo ◽  
Gilberto Sammartino ◽  
Jean-Baptiste Charrier
Keyword(s):  
Growth Factor ◽  
Cell Cultures ◽  
Human Cell ◽  
Platelet Rich Plasma ◽  
Platelet Rich Fibrin ◽  
Human Cell Cultures ◽  
Growth Factor Release ◽  
Factor Release
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