scholarly journals Heparin‐regulated growth factor release in vitro and angiogenesis in vivo from hyaluronan hydrogels

2007 ◽  
Vol 21 (5) ◽  
Author(s):  
Daniel B. Pike ◽  
Shenshen Cai ◽  
Kyle R. Pomraning ◽  
Matthew A. Firpo ◽  
Xiao Zheng Shu ◽  
...  
Keyword(s):  
Growth Factor ◽  
Growth Factor Release ◽  
Release In Vitro ◽  
Factor Release

scholarly journals Biofunctionalization of porcine-derived collagen matrices with platelet rich fibrin: influence on angiogenesis in vitro and in vivo

2020 ◽  
Vol 24 (10) ◽  
pp. 3425-3436 ◽  
Author(s):  
Sebastian Blatt ◽  
Valentin Burkhardt ◽  
Peer W. Kämmerer ◽  
Andreas M. Pabst ◽  
Keyvan Sagheb ◽  
...  
Keyword(s):  
Growth Factor ◽  
Immunohistochemical Staining ◽  
Collagen Matrices ◽  
Platelet Rich Fibrin ◽  
Angiogenic Potential ◽  
Branching Points ◽  
Growth Factor Release ◽  
Factor Release

Abstract Objectives Porcine-derived collagen matrices (CM) can be used for oral tissue regeneration, but sufficient revascularization is crucial. The aim of this study was to analyze the influence of platelet-rich fibrin (PRF) on angiogenesis of different CM in vitro and in vivo. Materials and methods Three different CM (mucoderm, jason, collprotect) were combined with PRF in a plotting process. Growth factor release (VEGF, TGF-β) was measured in vitro via ELISA quantification after 1,4 and 7 days in comparison to PRF alone. In ovo yolk sac (YSM) and chorion allantois membrane (CAM) model, angiogenic potential were analyzed in vivo with light- and intravital fluorescence microscopy after 24 h, then verified with immunohistochemical staining for CD105 and αSMA. Results Highest growth factor release was seen after 24 h for all three activated membranes in comparison to the native CM (VEGF 24 h: each p < 0.05; TGF-β: each p < 0.001) and the PRF (no significant difference). All activated membranes revealed a significantly increased angiogenic potential in vivo after 24 h (vessels per mm2: each p < 0.05; branching points per mm2: each p < 0.01; vessel density: each p < 0.05) and with immunohistochemical staining for CD105 (each p < 0.01) and αSMA (each p < 0.05). Conclusions PRF improved the angiogenesis of CM in vitro and in vivo. Clinical relevance Bio-functionalization of CM with PRF could easily implemented in the clinical pathway and may lead to advanced soft tissue healing.

Bone-derived growth factor release from poly(α-hydroxy acid) implants in vitro

Biomaterials ◽  
1993 ◽  
Vol 14 (3) ◽  
pp. 177-183 ◽  
Author(s):  
M.C. Meikle ◽  
W.-Y. Mak ◽  
S. Papaioannou ◽  
E.H. Davies ◽  
N. Mordan ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hydroxy Acid ◽  
Growth Factor Release ◽  
Factor Release

428 GROWTH FACTOR RELEASE FROM A CHEMICALLY MODIFIED ELASTOMERIC POLY (DIOL CITRATE) SCAFFOLD PROMOTES ANGIOGENESIS IN VIVO

2011 ◽  
Vol 185 (4S) ◽  
Author(s):  
David Kollhoff ◽  
Matthew Bury ◽  
Natalie Fuller ◽  
Partha Hota ◽  
Natalie Tapaskar ◽  
...  
Keyword(s):  
Growth Factor ◽  
Chemically Modified ◽  
Growth Factor Release ◽  
Factor Release

scholarly journals 4D printing of stretchable nanocookie@conduit material hosting biocues and magnetoelectric stimulation for neurite sprouting

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Jen-Hung Fang ◽  
Hao-Hsiang Hsu ◽  
Ru-Siou Hsu ◽  
Chih-Kang Peng ◽  
Yu-Jen Lu ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Fate ◽  
Electromagnetic Stimulation ◽  
Frequency Magnetic Field ◽  
Growth Factor Release ◽  
Differentiation And Proliferation ◽  
Dimensional Printing ◽  
Proliferation In Vitro

Abstract A high-frequency magnetic field (MF) generates an electric current by charging conductors that enable the induction of various biological processes, including changes in cell fate and programming. In this study, we show that electromagnetized carbon porous nanocookies (NCs) under MF treatment facilitate magnetoelectric conversion for growth factor release and cell stimulation to induce neuron cell differentiation and proliferation in vitro and in vivo. Integrating four-dimensional printing technology, the NCs are exposed on the surface, which enhances the cell adhesion and allows direct manipulation of electromagnetic stimulation of the cells. Remarkably, large amounts of growth factor encapsulated in NC@conduit resulted in excellent permeability and on-demand release, improving the in vivo layers of myelin sheaths and directing the axon orientation at 1 month postimplantation. This study offers proof of principle for MF-guided in vivo neuron regeneration as a potentially viable tissue regeneration approach for neuronal diseases.

Development of a dual growth factor loaded biodegradable hydrogel and its evaluation on osteoblast differentiation in vitro

2011 ◽  
Vol 1312 ◽  
Author(s):  
Deepti Dyondi ◽  
Thomas J Webster ◽  
Rinti Banerjee
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Osteoblast Differentiation ◽  
Chitosan Nanoparticles ◽  
Marker Genes ◽  
Tissue Engineering Scaffolds ◽  
Biodegradable Hydrogel ◽  
Growth Factor Release ◽  
Factor Release

ABSTRACTHydrogels with their tunable properties are attractive candidates for developing tissue engineering scaffolds for various applications (including bone and cartilage). The current work involved studying the synergistic effect of basic fibroblast growth factor (bFGF) and platelet derived growth factor BB (PDGF-BB) entrapped within injectable porous gels for bone regeneration applications. An in situ gelling system was developed using bacterial polysaccharides gellan and xanthan gum by temperature and ionic gelation with Ca+2. After the initial characterization of the hydrogels, a dual growth factor release system was developed wherein growth factors were encapsulated within chitosan nanoparticles embedded in the gels as well as directly within the gel. The hydrogel structure was characterized by SEM and TEM and in vitro growth factor release studies showed a slow release profile in PBS. Further, human fetal osteoblasts were entrapped within the hydrogel and a 21 day osteoblast differentiation study was conducted. An improvement in osteoblast total protein synthesis and collagen content was observed by day 21 compared to control gels without growth factors. Although further evaluation regarding mechanical properties and expression of osteogenic differentiation marker genes will be necessary, the present study suggests that injectable scaffolds can be used for the delivery of multiple growth promoting agents to support osteoblast differentiation.

scholarly journals Nanodiamond-based injectable hydrogel for sustained growth factor release: Preparation, characterization and in vitro analysis

2017 ◽  
Vol 58 ◽  
pp. 479-491 ◽  
Author(s):  
Settimio Pacelli ◽  
Francisca Acosta ◽  
Aparna R. Chakravarti ◽  
Saheli G. Samanta ◽  
Jonathan Whitlow ◽  
...  
Keyword(s):  
Growth Factor ◽  
Injectable Hydrogel ◽  
Sustained Growth ◽  
Release Preparation ◽  
Growth Factor Release ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Factor Release

1851 GROWTH FACTOR RELEASE BY LUNG CELLS IN VITRO IN RESPONSE TO HYPEROXIA

1985 ◽  
Vol 19 (4) ◽  
pp. 419A-419A ◽  
Author(s):  
M Tzaki ◽  
P Byrne ◽  
K Tanswell
Keyword(s):  
Growth Factor ◽  
Lung Cells ◽  
Growth Factor Release ◽  
Factor Release
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