Influence of temperature on the production of an archaeal thermoactive alcohol dehydrogenase from Pyrococcus furiosus with recombinant Escherichia coli

Extremophiles ◽  
2006 ◽  
Vol 10 (3) ◽  
pp. 221-227 ◽  
Author(s):  
Jürgen Kube ◽  
Christian Brokamp ◽  
Ronnie Machielsen ◽  
John van der Oost ◽  
Herbert Märkl
2012 ◽  
Vol 15 (4) ◽  
pp. 797-798
Author(s):  
G. Wroński ◽  
K. Budzińska ◽  
B. Szejniuk ◽  
A. Jurek

Abstract The aim of the study was to determine the influence of temperature, i.e. 4 and 20oC on the Escherichia coli O157:H7 survival time in a stored cattle slurry in a laboratory model experiment. The results of this investigation indicated that the tested microorganisms underwent a gradual elimination in the cattle slurry, whereas their inactivation rate was clearly dependent on the temperature. A higher survival rate was found in Escherichia coli O157:H7 at 4oC where a theoretical survival time of these microorganisms, determined using a regression analysis, amounted to 83 days. Our study indicates that there is a necessity for the slurry to undergo hygienization processes and that a constant monitoring of liquid animal excreta in search for pathogenic microorganisms is required.


2017 ◽  
Vol 6 (1) ◽  
pp. 20-23 ◽  
Author(s):  
Md Shaheduzzaman ◽  
Md Sayedur Rahman ◽  
Ifra Tun Nur

The cellular response against environmental stresses is one of the most highly conserved regulatory features among all organisms. The exposure of cells to stresses such as heat shock leads to the accumulation of partially and fully denatured proteins that interfere with normal cellular function. Present study was designed to examine the growth and physiology of Escherichia coli at different temperatures in our laboratory condition. With a previous observation of Escherichia coli growth cessation by the increase in temperature on different culture media, current study further extended the examination of the influence of temperature on the growth behavior of fecal coliform on minimal media, a slight retardation in the colony and cell morphology was noticed for fecal coliform at 47 °C within 36 hours to 72 hours of incubation. Consistent result was also found in spot test for fecal species at 45°C.Stamford Journal of Microbiology, Vol.6(1) 2016: 20-23


1992 ◽  
Vol 108 (1) ◽  
pp. 115-121 ◽  
Author(s):  
J. Dolman ◽  
R. G. Board

SUMMARYThe inner membrane of the air cell of hens' eggs was inoculated with Pseudomonas putida, Staphylococcus xylosus, Enterococcus faecalis, Escherichia coli and Salmonella enteitidis.The first mentioned eventually dominated the contamination of the albumen of eggs stored at 4, 15, and 20 °C. The last mentioned did so in eggs stored at 37 °C. The interval between inoculation of the membrane and gross contamination of the albumen was markedly influenced by site of contamination relative to yolk movement.


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